荧光的

P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.

丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。

Only sonoluminescence energy has not enough energy to activate hematoporphyrin, but more hematoporphyrin will be activated when sonoluminescence is existing.

血卟啉被激活的同时，若有大量生理盐水声致荧光的存在，血卟啉的激活量将大大增加。

Chapter 1: The principle and the development of intramolecular charge transfer fluorescence were described.

第一章：本章简要概述了分子内电荷转移荧光的原理及研究现状、蛋白质光谱探针的研究进展和DNA光谱探针的研究进展。

Light response curve of photosynthesis and some parameters of chlorophyll fluorescence in these species were determined.

分别将马尾松、黧蒴、荷木、黄果厚壳桂的幼苗置于100％自然光和32％自然光下生长6个月，测定它们的光强-光合反应曲线和叶绿素荧光的某些参数。

Hereinto, Tyr-Fal was prepared from L-tyrosine and formaldehyde under alkaline conditions.Firstly, a new multi-functional film with well-defined layered structure was fabricated from Tyr-Fal and PEI via LbL method. Experiment revealed that this Tyr-Fal/PEI multilayer film emit yellow fluorescence under UV radiation. The ability of the Tyr-Fal/PEI multilayer film for adsorbing rosaniline improved after UV-exposed, which can be used in preparing micropattern.

然后以Tyr-Fal为结构单元，通过静电力自组装构筑了两种功能性的酪氨酸基多层膜：(1)利用Tyr-Fal与聚乙烯亚胺间的静电力构筑了结构稳定、有序、能在紫外光照射下发出耀眼黄色荧光的聚乙烯亚胺/酪氨酸缩甲醛超薄膜。

The characteristics not only provide a possibility to separate these different configurations spectrally but also present a importance for directly investigating the properties of hole burning of different configurations.

通过分析荧光的时间特性和利用激发光谱，光谱烧孔等光学手段，首次发现处于不同配位环境的二价钐离子具有差别很大的〓无辐射弛豫速率。

The participant manner and the interferential mechanism of the adscititious reagents are different each other.

归纳得到系列Me〓参与形成药物-BSA-Me〓复合物的稳定性、药物对BSA内源荧光的猝灭效应等作用参数按照Me〓种类的排列次序。

Darkness in Antarctica's Weddell Sea gives this comb jelly a chance to show off its candy-colored bioluminescent cells.

黑暗在南极洲的威德尔海给这栉水母偶然发生去炫耀它的糖果—染色的生物荧光的细胞。

"Nocturnal crawler, a scorpion remains well camouflaged under a flash-light's beam, but fluoresces in ultraviolet light , a reaction that remains a mystery to scientists."

夜间爬行者，一只蝎子很会伪装在手电筒的光线，但是发荧光的紫外线，一种反应遗留一种神秘的事物留给科学家。

As their fluorescence emissions are size-tunable, we can acquire any spectrum from ultraviolet to near-infrared by changing the particles＇ radiuses.

量子点发射荧光的可调节性强，通过改变粒子半径的大小可获得从紫外到近红外范围内任意点的光谱，且Stokes位移大。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。