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Have tried to expand the definiens of CL, analysed and explained the phenomena and rules of CL, analysed the schemes for detect CL by overseas scholars and made a suit of generalized CL detect and analysis system by myself.

主要是扩展了阴极荧光的定义,分析解释了阴极荧光现象和规律,分析了国外阴极荧光探测方案的不足,设计制造了广义阴极荧光分析系统,实际测试了几百个样品,并有一批新发现。

The source of photoluminescence was discussed and we speculate that it was caused by fluorescence resonance energy transfer between the tryptophan of PrP and hematoporphyrin in the bacteria.

同时对荧光来源进行推理并——验证,最后推测导致朊蛋白有荧光的原因是,朊蛋白中的色氨酸与细菌中荧光小分子血卟啉发生荧光共振能量转移。

The objectives of the present study were to evaluate the feasibility of rhodamine B hydrazide as a probe for peroxynitrite and its potential use in biological systems.

RBH本身为个无色、无荧光的物质,ONOO〓与RBH反应生成强荧光产物罗丹明B,导致体系的荧光强度大大增强。

To investigate the fluorescence dynamic change of protoporphyrin IX in bladder carcinoma cell induced by 5-ALA and the influence of 5-ALA concentration and pH on fluorescence of PpIX. 2. To discuss the biodistribution and express of PpIX in bladder wall of different lesions by method of instilling of 5-ALA into bladder cavity. 3. To discuss the clinical value of the fluorescence diagnosis in early detection of bladder cancer and the radicality of bladder tumor resection under fluorescence cystoscopy.

本研究目的:1了解5-ALA诱导膀胱癌细胞内生原卟啉IX荧光随时间的动力学变化及5-ALA的浓度、pH值对产生PpIX荧光的影响。2通过膀胱灌注5-ALA探讨其所诱导内生PpIX荧光在膀胱上皮中分布及不同病变表达上的差异。3探讨5-ALA诱导荧光膀胱镜检对发现早期膀胱癌的临床应用价值及在荧光膀胱镜下膀胱肿瘤电切治疗的彻底性。

Both density and intensity of fluorescence in SVitC and LVitC groups were lower than those of control group. There were much wider fluorescence stripe and strengthened intensity in IVIG and IVIG+VitC groups. The myofilaments were in wild disorder and sarcomere had severe breakage in control group.

5心肌的免疫荧光检测:对照组比较粗的荧光条带主要集中在心肌间质,大、小剂量VitC组荧光的密度和强度有所降低,而IVIG组和IVIG+VitC组的荧光条带增宽,在心肌间质中呈宽大的条索状分布,亮度明显增强。

RESULTS The SOD activity had linear correlation with pH in the range of pH 2.2-8.0 when SOD concentration was fixed. The varication of activity after ultrosonication had linear correlcation with fluoresent intensity at 340 nm. With increase of uhtrosonic frequency, the microenviroment of tyrosine and tryptophan inside the SOD molecule was changed, then the balance of hydrophokicity and hydrophilicity inside the molecule was broken, this induced the blue shift of synchronous spectrum band and decrease of SOD activity.

结果 SOD浓度一定、pH2.2~8.0时,活性与其线性相关,超声后活性差值与340 nm荧光强度差值的线性相关;随着超声次数的增加,蛋白质SOD分子内部的酪氨酸、色氨酸所处的微环境被扰动,打破了分子内部的疏水和亲水平衡,使蛋白质的活性降低,在荧光检测中观察到普通荧光谱带蓝移,同步荧光的荧光峰位总体呈现峰位蓝移和强度下降。

The experiment showed that the twin spirochaeta of PNA-DNA can quench the fluorescence of ST in some degree.And the fluorescence quenching of ST was reduced when PNA-DNA twin spirochaeta dissociate.

实验结果发现当 PNA- DNA杂交形成双链时,可以在一定程度上猝灭 ST的荧光,而当双螺旋解链时,对 ST荧光的猝灭程度减小,逐渐接近于 ST本身的荧光,根据荧光强度的变化研究了 PNA- DNA的杂交和解链过程。

The intensity ratio of TO and LO inMCT was observed to be different. Such difference was explained in terms of the different Ramangeometry arrangement.〓. The laser-induced micro-photoluminescence in the range of 1000~5000〓(1.34eV~1.83eV) was found for the first time in LPE MCT epilayer. The center of photoluminescence wasat 2750〓 or 1.62eV and the FWHM of luminescence was 2000〓 or 0.25eV. We assume thatthe photoluminescence is due to recombination of electron from an anion vacancy resonance levelto the top of valance. In addition, new Raman shift was observed at 750〓 in LPE MCTepitaxial film.〓. The laser-induced micro-photoluminescence with quasi-periodic structure was observed forthe first time at room temperature in one of MOVPE MCT epitaxial film samples. The range offluorescence was from 1.46eV to 2.21eV, i.e., 1.73eV above the conduction band edge.

2首次在LPE生长的碲镉汞外延薄膜的显微Raman谱中,在1000~5000〓范围发现了激光激发显微荧光,该荧光的发光范围换算为电子伏特标度为1.34eV~1.83eV,荧光的发光中心大约位于2750〓,即1.62eV,发光的半峰高宽约为2000〓或0.25eV;指出该显微荧光来源于碲镉汞薄膜中的阴性离子空位共振能级的激光激发发光;观察到了碲镉汞外延薄膜中一个新的Raman散射峰,位于750〓位置; 3首次在一块用MOVPE方法生长的〓Te外延薄膜的显微Raman谱中,发现了1.46eV至2.21eV范围并伴随有周期结构的显微荧光峰,该发光峰对应的能带中心位于〓Te材料导带底上方1.73eV,通过研究得出样品在1.46eV至2.21eV范围的显微荧光峰是由于改进 MOCVD 生长工艺,提高了碲镉汞外延薄膜的结构质量所致;通过分析指出该显微荧光来源于外延层中的阴性离子空位的共振能级发光。

The product has green-yellow fluorescence at excitation wavelength of 355 nm and emission wavelength of 478 nm, and the fluorescence intensity could be enhanced in the presence of acetone.

三、流动注射在线氧化荧光法测定氯普噻吨的的研究利用在硫酸介质中二氧化铅可使无天然荧光的氯普噻吨氧化而转变为在最大激发波长和最大发射波长分别为393nm和452nm的强荧光物质,而且丙酮对该荧光体系有强烈增敏作用的现象,建立了以醋酸纤维素固定化的二氧化铅作为固相反应器的流动注射在线氧化荧光法测定氯普噻吨的新方法。

It applies the Fourierism-Gauss spectrum analysis on the in-situ X-ray instrument and advances the precision and the area of in-situ X radiation sampling technique.

本文的研究是对现场X荧光分析技术的一项补充,将原本在微机上使用解谱技术应用到现场X荧光分析中,提高X荧光的测量精度,扩大X荧光的应用范围。

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