荧光性

Objective To study the advantages of dual-color fluorescence in situ hybridizationin sex chromosome analysis of gonad agenesis patients over traditional chromosome analysis.

目的 探讨荧光原位杂交技术比常规染色体分析，在性腺发育不全患者性染色体检测中的优越性。

There are dispersing agents, lubricants, preservatives, fluorescence, waterproof protective agent, as well as ink absorption of dyes, and ameliorant, and so on, mainly in buyang pigments and glue are insufficient, and reduce operations on them.

有分散剂、润滑剂、防腐剂、荧光剂、耐水化剂、以及染料、油墨吸收性及改良剂等，其主要作用在补颜料与接着剂所不足者，并减少操作上之困扰。

This is mainly due to that pyrene ligand has better planar and aromatic character than anthracene.

这主要是由于配体芘比蒽具有更好的芳香性和平面型，可以使探针Ru-2更好的与DNA结合，使荧光增强。

We analyse the adjustment of the birch reeds to the cold and drought from the conductance, K+ filtrate, SOD, POD, MDA, content of the water-soluble protein, penetration of the bioplasm film, photosynthesis and fluorescence.

3通过对欧洲优良无性系在寒冷、干旱的胁迫下电导率、钾离子的渗透率、酶促防御系统，膜质过氧化物，可溶性蛋白含量、原生膜透性和光合、荧光等生理指标的变化，对欧洲白桦优良无性系苗期的适应性进行分析。

The intralellular labeling methods developed for single cell analysis are summarized in this review, including cell itself acting as a microreaction chamber for derivatization, dericatization mediated by liposome or polyethylene glycol to increase permeabilization of cell membrane, on- and post-column derivatization during single cell analysis with capollary electrophoresis or microchip electrophoresis and labelling of cells with quantum dots.

本文综述了在单细胞分析中常用的荧光标记方法，包括细胞作为微反应器的衍生法，借助于脂质体与聚乙二醇等增加细胞膜通透性的衍生方法和在毛细管/芯片毛细管电泳分析单细胞时柱上衍生和柱后衍生法以及量子点的标记法等。

To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主，要求研究生学习和掌握一些现代微生物学研究所需的基本技术，内容包括：相差显微镜、荧光显微镜的使用和显微摄影技术，PCR技术的应用，细菌质粒的构建、转化及检测技术，病毒的侵染性及其分子检测技术，丝状真菌原生质体融合技术，大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术，细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

The absorption peak and PL emission peak of PPV-PQ in the solid state, were shift to long wavelengths compared to their corresponding peaks in solution due to strong inter-chain interactions and increased coplanarity.

结果显示，由于在固态下存在较强的分子间作用，分子链的共平面性增加，所以PPV-PQ无论是紫外-可见吸收，还是荧光发射光谱，在固态薄膜下都比在氯仿溶液里发生明显红移。

Methods: Firty eight patients with prostate cancer and 112 healthy cases were selected as the control randomly. NAT2 and CYP1A1 gene polymorphisms were analysed with the methods of PCR RFLP, ASA and real time fluorescence Light Cycler.

应用PCR RFLP、ASA和自动实时荧光定量分析技术，分析 5 8例前列腺癌病人和 112例健康对照者CYP1A1和NAT2基因 4个位点的多态性，比较前列腺癌病人与对照组间频率差异。

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

Methods: Forty-three indiviuals who were exposed to arsenic more than 0.05mg/L in well water were chosen and the vein blood and morning urine was collected; The MTHFR C677 polymorphism was analyzed by PCR-RFLP method; Urinary inorganic and methylated arsenic were speciated by high performance liquid chromatography combined with hydride-generation atomic fluorescence spectrometry; Microbiological assay was used to estimate serum folate.

在砷中毒流行区选择饮水史清楚、饮水砷含量高于0.05mg/L的43名居民，采集空腹静脉血并收集晨尿，以聚合酶链反应－限制性长度多态性方法分析MTHFR基因C677T位点多态性，采用高效液相色谱－氢化物发生－原子荧光法检测尿中各种形态的砷，采用微生物法测定血清中叶酸水平。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。