荧光性

To construct a retroviral vector carrying immortalizing gene hTERT, screen gene puro, reporter gene EGFP and recombination site sequence LoxP for reversible cell immortalization. Linearized pBABE-puro was cut by restriction enzyme Nhe Ⅰ and ligated with loxP double strands to construct a vector pBABE-puro-lox.

目的 探讨以hTERT作为永生化基因、嘌呤霉素乙酰转移酶基因puro作为筛选基因、加强型绿色荧光蛋白EGFP作为报道基因、loxp作为重组位点构建可回复性永生化逆转录病毒载体，为建立可回复性永生化细胞株莫定基础。

The genetic relationship of 27 sapodilla cultivars and 4 mamey sapoae cultivars were analyzed by using fluorescent-labeled AFLP markers. A total of 1131 AFLP markers between 75 to 500 base pairs were generated with 9 primer combinations, of which 1096 (96.9%) were polymorphic.

利用荧光AFLP分子标记技术对27个人心果品种及4个蔓妹人心果品种资源的亲缘关系进行研究。9对引物共扩增得到1131条带(75~500 bp)，其中多态性带1096条(96.90%)。28个品种具特征性条带，特征带大小为350~500 bp。

New chiral boronic acid sensor was synthesized and its enantioselectivity towards tartaric acid was studied.

合成了新的由蒽及苯乙胺衍生的手性芳基硼酸荧光探针，研究了该手性探针对酒石酸的对映选择性识别作用。

The neuronal connections of the nucleus accumbens in the rat was studied with aid of retrograde and anterograde tracers.

用HRP和荧光素—伊凡氏兰、核黄对大白鼠伏核的传入性联系，用WGAHRP对其传出性联系进行了实验研究。

The chiral polymer P-1 shows strong blue fluorescence due to the extended π-electronic structure between the chiral repeating unit-6,6'-di(4-trifluoromethyl-phenyl)-2,2'-bisoctoxy-1,1'-binaphthyl and the conjugated linker 2,2'-bipyridyl group via vinylene bridge.

手性高分子P-1能发射强的蓝色荧光，这是由于手性重复单元-6，6′-二(4-三氟甲基苯基)-2，2′-二正辛氧基-1，1′-联萘和单元2，2′-联吡啶通过亚乙烯基桥连形成共轭高分子结构造成的。

Results show that, by uv-vis spectra, fluorescence spectra and Fourier infrared spectra transform all successfully gained water-soluble CdTe quantum dots. With the increase of water-soluble CdTe quantum dot concentration increasing, Achyranthes Bidentata root length and the seed vigor reduced ordinally, but its germination changed no obviously, suggested the space mutagenesis treatment can improve Achyranthes Bidentata seed vigor.

结果表明，紫外－可见光谱、荧光光谱和傅立叶红外变换光谱图均说明已成功制备水溶性CdTe量子点；随水溶性CdTe量子点浓度增大，牛膝的根长和种子活力依次降低，但其对发芽势影响的规律不明显，经空间诱变处理能提高牛膝种子活力。

In order to evaluate prevalence of Chlamydia psittaci in Beijing and other provinces around, 374 blood samples and 81 clinical suspected samples from Beijing, Hebei, Tianjin, Nei Monggol, Shandong, Shanxi and Henan were investigated by detecting for antibodies against C.psittaci using IHA and ELISA, and for pathogens using modified Ziehl-Neelsen staining and immunofluorescence tests.

对北京市周边6省份怀疑感染鹦鹉热嗜性衣原体的鸡鸭血清样品374份、病料81份，分别使用IHA诊断试剂盒、ELISA试剂盒以及抗酸染色试剂和荧光抗体诊断试剂进行了检查，以评价北京市及其周边地区家禽鹦鹉热嗜性衣原体的流行性。

Both the rat encephaledema of BS and BC were developed by intracranial injection of sludged blood to lead to hematom for observing the changes of the brain coefficient,water cont...

脑血肿组和黄芩甙组采用自体血制作脑出血模型，3组大鼠均测定脑系数并用干湿法测脑组织水分，同时病理镜检观察脑出血组和黄芩甙组脑组织变化，并采用高效液相色谱荧光法测定兴奋性氨基酸脑组织谷氨酸和抑制性氨基酸γ氨基丁酸含量变化。

The pollen living ability declined with time prolonging and the best condition for pollination was 22~28℃and fresh pollen appearing .By means of benzidine- hydrogen peroxide reaction and aniline blue fluorescence method, showed that the stigma receptivity could teach to 12 days since flowering. Compared with self pollination, the pollen was easily adhered into sigma and the pollen tube was easily entered into style in cross pollination experiment.

通过联苯胺—过氧化氢反应法和苯胺蓝荧光法观察了柱头的可授性、花粉在柱头的黏附、萌发及花粉管的生长情况，结果表明：非洲菊柱头发育初期就具有可授性，可授期达12d ；杂交和自交相比，杂交的花粉比较容易黏附、花粉管进入柱头比较容易，二者存在花粉黏附数量和花粉管进入数量的差异。

Objective To discuss the value of detection of serum serum anti-nuotrophil cytoplasmic autoantibodies and anti-vascular endothelial cell antibodies in the diagnosis of erythema nodosum. Methods Samples from erythema nodosum patients and normal people were collected, the ANCA and AECA were detected by indirect immunofluorescence.

目的 探讨血清抗中性粒细胞胞浆抗体及抗血管内皮细胞抗体在结节性红斑发病中的作用和意义方法收集结节性红斑患者和正常人血清，用间接免疫荧光技术检测血清中ANCA和AECA，并分型。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。