荧光性

The RNA from the same subject was divided into two aliquot and labeled with Cy3 and Cy5 respectively. The spots with significant difference between the foreground and local background intensities and those without significant difference between Cy5 and Cy3 signal intensities were selected for analysis.

排除阴性和阳性对照信号值、Cy3和Cy5的前景与背景间无统计显著性差异点的信号值，进一步排除两种荧光标记间存在统计显著性差异点的信号值，将3张芯片的共同信号值用于分析。

Fluorescence, UV-vis and NMR are the most common methods in the research of chiral recognition. According to these methods, the latest research progress in the synthesis of chiral receptors based on calix[4]arenes and their applications in the chiral recognition are summarized.

在手性识别的研究中，荧光、紫外和核磁是3种最常见的研究方法，本文根据这3种方法进行分类，综述了近年来以杯[4]芳烃为分子骨架的手性受体的合成及其在手性识别中的应用。

By blast in GenBank, clone Y1218 shared 72% homology with Chromobacterium violaceum, clone Y1814 shared 88% homology with Pseudomonas putida,clone Y0140, 80% with Bacillus sp., and clone Y1038, 71% with Pseudomonas fluorescens. Some clones had homology with the degreadation of aniline, methyl parathion microbial organisms.

有80%的同源性，Y1038克隆和荧光假单孢有71%的同源性，有部分克隆和一些具有降解苯胺、甲基对硫磷等有机物的微生物有同源性。

The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro. Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light.

结果：(1)通过细胞培养和MTT法，表明长白山姬松茸在体外对MGC-803细胞株有明显的抑制作用，加药组与对照组相比其生长抑制率有显著性差异(P＜0.01)，而且这种抑制作用呈现浓度和时间的依赖性；(2)通过集落形成率的测定结果表明，加药组与对照组相比其集落形成率和集落形成抑制率有明显差异(P＜0.01)，说明长白山姬松茸对MGC-803胃癌细胞株有明显抗增殖作用；(3)光镜观察结果表明，加药组可见细胞脱水浓缩伊红染色增强，胞体缩小，内含高度浓缩的胞核呈深蓝色等典型细胞凋亡形态；经AO／EB荧光染色观察结果表明，当终浓度为1.0mg／ml的姬松茸提取物作用于MGC-803胃癌细胞24h后，其凋亡率和破膜率与自然凋亡率与破膜率相比，均有显著性差异，其凋亡率86.3％(P＜0.001)，破膜率为41.6％(P＜0.01)，说明姬松茸确实有诱导MGC-803胃癌细胞凋亡的作用，同时也有细胞毒作用，但以诱导细胞凋亡为主；(4)免疫组化结果表明，用药前后凋亡相关基因的BCl-2、Bax蛋白均有显著性的改变(P＜0.001)。

In this paper, we summarize the synthetic methods, structure features and photophysical properties of WSCPs developed in recent ten years, and their applications in a new generation of optoelectronic devices and bio-chemical detection. On this basis, we point out the existent problems in the present research and applications of WSCPs,and also give a perspective on their future.

本文总结了近10年来报道的水溶性共轭聚电解质的结构特点和合成方法，以及对不同化学或物理条件下光物理性质的研究，归纳了它们在新一代光电器件制作和荧光传感中的应用，并在此基础上提出了水溶性共轭聚电解质研究中尚待解决的问题，并展望了水溶性共轭聚电解质的应用前景。

Results: 1. The value of all experimental indexes decreased significantly after heat stress, and reached the lowest at the time 2-8 hours after heat stress. This indicates that the immunity of the mice is damaged to the largest extent at 2-8 hours after heat stress. 2. L-arginine supplementation with appropriate dose could remit the acute atrophying of thymus and spleen tissue caused by heat stress. 3. After L-arginine supplementation with appropriate dose, the lymphocyte proliferation, the level of concentration of IL-2 and * expression of IL-2R raised in the group with room temperature; in the heat stress group the level of three indexs decrease significantly. Furthermore, the decrease of the group given 1 .Smglg.bw L-arginine is the smallest. This result indicates that the concentration of 1 .Smglg.bw L-arginine supplementation could remit the depressment of the immunity caused by heat stress. 4. The [Ca2~] in activated thymocytes of the group with L-arginine -2- supplementation is significantly higher than that of the group with water supplementation. This indicates that L-arginine supplementation could protect thymoeytes of mouse under heat stress. We also found that the fluorescence intensity of [Ca2~] in activated thymocytes of the group given 1 .Smglg.bw L-arginine is the highest.

结果：1、热应激后小鼠的各项免疫指标均有显著性下降，其中在2.8小时降到最低，提示在这个时间段小鼠的免疫功能受到最大损害；2、适量补充精氨酸有助于缓解热应激导致小鼠胸腺和脾脏的急性萎缩；3、常温组，补充适量精氨酸后小鼠淋巴细胞增殖活性、IL-2的浓度及IL-2R的表达均有显著性上升，而热应激后三指标均显著降低，其中在精氨酸给予浓度为1.5mg/g.bw时三指标的降低程度最少，提示该浓度可最大程度缓解热应激对小鼠免疫功能的抑制；4、补充精氨酸组的活化胸腺细胞［Ca~(2+)］较给水组有显著性上升，说明精氨酸对热应激小鼠胸腺细胞具有保护作用，并发现在精氨酸给予浓度为1.5mg/g.bw时活化胸腺细胞的胞浆钙离子荧光强度达到最大；5、热应激后小鼠血清NO的浓度均有不同程度的上升，补充精氨酸后血清NO上升更加明显，但并非随精氨酸给予浓度的增加而增加。

Methods Samples from 78 patients with pituitary adenomas, including 37 invasive and 41 noninvasive, and 5 normal pituitary tissue specimens from autopsied patients were examined. The expressions of OPN mRNA and OPN were examined by real-time fluorescence quantitative PCR and immunohistochemical SP method, respectively.

分别对78例垂体腺瘤手术标本（其中侵袭性37例，非侵袭性41例）和5例来源于尸检的正常垂体组织进行检测，采用实时荧光PCR技术检测组织中OPN mRNA表达，免疫组织化学SP法检测OPN蛋白表达，结合临床资料分析OPN mRNA及其蛋白的表达与垂体腺瘤侵袭性的关系。

Methods samples from 78 patients with pituitary adenomas, including 37 invasive and 41 noninvasive, and 5 normal pituitary tissue specimens from autopsied patients were examined. the expressions of opn mrna and opn were examined by real-time fluorescence quantitative pcr and immunohistochemical sp method, respectively. the relation of opn and opn mrna expressions to invasiveness of pituitary adenomas was analyzed.

分别对78例垂体腺瘤手术标本（其中侵袭性37例，非侵袭性41例）和5例来源于尸检的正常垂体组织进行检测，采用实时荧光pcr技术检测组织中opn mrna表达，免疫组织化学sp法检测opn蛋白表达，结合临床资料分析opn mrna及其蛋白的表达与垂体腺瘤侵袭性的关系。

Therefore, the EL spectra of (Znq_2)_4 is wider than that of Znq_2. 4._2 was synthesized. The analysis of molecular spatial structure and the characterization of material performance of_2 and Liq showed that two Liq molecules and two Naq molecules were connected by Na-O-Na bond bridges to form_2. Compared with Liq,_2 exhibits stronger rigidity in planar molecular structure, larger steric hindrance and intermolecular distance, and much smaller molecular polarity, thus resulting in much longer fluorescence lifetime, much higher fluorescence quantum efficiency, wider energy bandgap and better film formability. When used as light-emitting layer in OLED,_2 shows lower formation probability of excited dimmer and exciplex formation than Liq, thus emits bluer light with higher current efficiency than Liq. When_2 ultrathin film is used as electron injection layer in OLED, it exhibits higher current density, higher luminance, lower turn-on voltage and higher current efficiency than Liq ultrathin film for the existence of sodium ions in_2 ultrathin film. 5. The summarization the relationship between molecular spatial structure and material performance of Alq_3,(Znq_2) and Liq, lead the conclusion that the molecular spatial structure of Mq_n affects its material performance in such aspects as the rigidity of planar molecular structure, intermolecular interaction, molecule stacking mode and intermolecular distance.

制备了_2，通过对_2和Liq的分子空间结构与性能进行分析与讨论，发现_2是通过两个Na-O-Na键桥将两个Liq和两个Naq连接构成的，其分子平面结构的刚性程度强于Liq，空间位阻大于Liq，分子之间的距离大于Liq，分子极性远远小于Liq；_2的荧光寿命长于Liq，荧光量子效率高于Liq，成膜性优于Liq；_2的禁带宽度比Liq大，光致发光光谱中_2的最大发射峰较Liq发生蓝移；当_2在OLED中作为发光层时，激发二聚体与激基复合物的生成几率远远小于Liq，发的光比Liq更蓝，电流效率大于Liq；_2超薄膜中有Na离子的存在，与Liq超薄膜相比，当其在OLED中作为电子注入层时，具有更大的电流密度，更高的发光强度，更低的阈值电压和更高的电流效率 5、对上述Alq_3、Znq_2和Liq的分子空间结构与材料性能之间的关系进行了归纳总结，认为Mq_n的分子空间结构主要在分子平面结构的刚性程度，相邻分子之间的相互作用，分子堆叠的方式和分子之间的距离这四个方面影响其性能。

The recombinant pThr-2 was expressed as intracellular insoluble inclusion bodies and must be refolded for the activity assay.

荧光光谱分析表明复性后得到的凝血酶具有与天然态凝血酶相同的最大荧光发射波长。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。