荧光性

The total amount of these three isomers could be measured when they were syn-chronously scanned at △λ=0 nm because they have homoplastic character of fluorescence spectrum and favourable additivity of fluorescence intensity in 350～500 nm.

而以λ=0nm进行同步扫描时，在350～500nm之间具有相似的荧光光谱特征，其荧光强度有良好的加和性，可以对三者进行总量测定。

Results: The normal epithelial cells were intactly lined with microvilli, and lesions on the surface of Ct infected cells were showed by SEM. The expression of actin was obvious throughout the cytoplasm of uterine epithelial cells but appeared to be most intense along the apical plasma membrane on day 4, 5 and 6 of pregnancy. On the day 7, levels of actin had increased along the apical surface. The expression of actin in the infected group was weaker than that in of the normal group.

结果：SEM示对照组子宫腔可见有微绒毛的分泌细胞；实验组腔上皮细胞表面有的可见破损，微绒毛少见；免疫组化结果显示荧光强度主要定位于腔上皮细胞胞质／胞膜，妊娠第4，5，6天沿胞质顶部分布较密集，而底部较弥散；妊娠第7天顶部强度有所增加；感染组的荧光强度均弱于相应正常组，在妊娠第5天存在显著性差异(p.05)。

The effects of low irradiance on chlorophyll a fluorescence kinetics were studied in two eggplant Solanum melongena L.

根据快速叶绿素荧光诱导动力学原理，选用耐弱光茄子品种99-8和不耐弱光茄子品种黑又亮作试材，进行遮光（光照强度相当于自然光照的40%）15 d、未遮光15 d、遮光30 d和未遮光30 d 4个处理，用JIP-测定法对不同耐弱光性品种在不同光照条件下的叶绿素荧光特性进行研究。

Objective To investigate the characteristics of fundus photography and fundus fluorescein angiography of IRVAN (idiopathic retinal vasculitis, aneurysms, and neuroretinitis) syndrome and Eales disease.

目的 观察特发性视网膜血管炎、动脉瘤、视神经视网膜综合征和Eales病荧光素眼底荧光血管造影特征的异同。

These novel indocyanines haveing good water solubility, fluorescent properties and photostability, can be used in biological analysis as fluorescent labeling reagents.

设计、合成的新荧光染料具有较好的水溶性、光稳定性和荧光性能，可望在生物分子标记、分析和分离等领域中得到重要应用。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

The method combined fluorogold retrograde with fluorescent immunohisto chemistry was used to study c-fos expression of the neurons of brian stem proje cting to the subnucleus reticularis dorsalis under noxious stimulation.

用荧光金逆行追踪和FOS荧光免疫组织化学染色相结合的方法对外周伤害性刺激下大鼠脑干内向延髓网状背侧亚核传入投射的神经元中c-fos的表达进行了研究。

To improve the stability of small unilamellar vesicles in vitro, the permeability of SUV from soybean phosphatidylcholine coated by collagen and chitosan was studied respectively by using 5(6)-Carboxyflourescein5(6-CF as a fluorescence probe.

为了提高小单层脂质体（SmalUnilamelarVesicles，SUV）的体外稳定性，以5（6）－羧基荧光素（5（6）－CF）为荧光探针，研究了脂质体被胶原蛋白和壳聚糖这两种天然生物大分子包覆后的通透性。

The change of agglutinating activity in different temperature and pH indicated that PML had no hemagglutinating activity at 80℃. The effect of chemical modification of amino acid residues upon hemagglutination was investigated. There was a definite decrease of fluorescence intensity which corresponds to the oxidation of tryptophan.

研究了不同温度、pH和变性剂对PML凝血活性的影响和荧光光谱的变化，当温度达80℃时，活性完全丧失；pH为4—9对活性影响不大，pH低于2和pH高于12时，大黑花芸豆凝集素的凝血活性大部分丧失；高温和强碱对荧光光谱有较大影响，表明PML对高温，强碱的耐受性不强。

Control eyes showed segmental distribution of tracer in the trabecular meshwork, with clustering of microspheres near collector channel ostia.

在对照眼，荧光微球在小梁网呈现节段性分布，沿房水丛内壁斑点状标记，在集合管口有荧光微球的聚集。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。