荧光性

Pyrene has been used as a fluorescence probe to investigate the associative behaviors of hydrophobically modified water-soluble terpolymers Poly(acrylamide/sodium acrylate/octylacrylamide) \[P(AM/NaAA/C_8AM)\].

采用胶束共聚共水解方法合成疏水改性水溶性聚合物聚(丙烯酰胺/丙烯酸钠/N 辛基丙烯酰胺)[P(AM/NaAA/C8AM)]，并以芘为荧光探针，应用稳态荧光光谱法研究了它的疏水缔合行为。

Tb3+ in DTPA-Tb is replaced by Np4+ giving DTPA-Np and Tb3+, then the free Tb3+ complex with Tiron producing Tiron-Tb which is a strong fluorescence substance. We use the fluorescence signal of the ligand to measure Np4+ in the system. The high sensitivity of Tiron-Tb makes the method for neptunium sensitive.

测定Tb~(3+)有一个极灵敏的方法，Tb~(3+)和Tiron结合生成强荧光的络合物Tiron-Tb，其检测下限可达4.8×10~(-12)g／ml Tb~(3+)，利用荧光法检测Tiron-Tb的高灵敏性，从而实现镎的间接测定。

The object of this study is to cancerous and normal cells intimacy of hematoporphyrin derivative for diagnosis and therapy cancer, fluorescence spectra of cancerous and normal cells are measured using a ultrashort pulses laser spectral technique and picosecond time-correlated single-photon counting system.

研究用于癌症诊断与治疗的光敏剂血卟啉(hematoporphyrin derivative， HPD)对人体癌细胞与正常细胞的亲和性，采用超短脉冲激光光谱技术和皮秒时间相关单光子计数系统，测量经血卟啉培养的活体癌细胞与正常细胞的荧光光谱，观测到癌细胞样品在创645nm处具有特有的光谱谱峰；测量不同浓度血卟啉荧光光谱，观测到随着血卟啉浓度的逐渐增大，在645nm处出现的光谱峰也随之显著增强。

The phycocyanin is a kind of fluorescence molecular probe because it has characteristic absorption spectra and fluorescence spectra.

藻蓝蛋白具有特征性吸收光谱和荧光光谱，是一种新颖的荧光分子探针。

Methods Fundus photograph,fundus fluorescein angiography and indocyanine green angiography were performed in a series of 70 patients with unilateral AMD and drusens and pigmentary changes in the macular region in contralateral eyes.

对70例一眼为湿性型AMD的患者对侧眼作眼底彩色照相、荧光素眼底血管造影（fundus fluorescein angiography，FFA）以及ICGA检查，并对其眼底荧光图像进行比较和分析。

The difference between the test groups and the control group was statistically significant. It could be deduced that all the insecticides tested in this experiment damaged the spiders′ intestinal mucosa, compromising its function as a barrier and letting large molecules such as FTIC-D more easily penetrate. Among the insecticides, pyrethrin and Bt preparation are bio-insecticides, and the FITC-D concentrations of the spiders exposed to these two bio-insecticides were more than those of the spiders exposed to the chemical insecticides imidacloprid and dipterex. The difference between the bio-insecticide groups and the imidacloprid group was significant, but there was no significant difference between the bio-insecticide groups and the dipterex group.

结果表明：4种杀虫剂处理后拟环纹豹蛛血液中的荧光素浓度远高于对照组，各个处理组拟环纹豹蛛血液样本中的荧光素浓度均与对照组存在极显著差异，可见4种杀虫剂均将对拟环纹豹蛛肠黏膜的屏障功能造成损伤，导致大分子物质易于通过；其中1.5%除虫菊素水乳剂三保奇花和Bt制剂强杀处理后拟环纹豹蛛中肠黏膜的通透性高于仿烟碱类化学杀虫剂吡虫啉处理组，差异达到显著水平，但与有机磷杀虫剂敌百虫处理组差异不显著。

Three-dimensional excitation emission matrix fluorescence(3DEEM) was used to investigate the characteristic fluorescence of dissolved organic matter s,such as humus,proteinoid,surfactant and vegetable tallow in the wastewater treatment process.

采用三维荧光光谱法研究了城市污水处理过程中溶解性有机物的三维荧光特性变化规律。

Three-dimensional excitation emission matrix fluorescence (3DEEM) was used to investigate the characteristic fluorescence of dissolved organic matters, such as humus, proteinoid, surfactant and vegetable tallow in the wastewater treatment process.

采用三维荧光光谱法研究了城市污水处理过程中溶解性有机物的三维荧光特性变化规律。

Effects of four insecticides on intestinal mucosa permeability of the wolf spider were studied by exposing spiders to insecticides and then measuring the concentration of fluorescein isothiocyanate-dextran in the spiders'blood. The results showed that FITC-D concentrations of the test groups were much more than that of the control group.

采用异硫氰酸荧光素-葡聚糖作为示踪物质，通过检测拟环纹豹蛛血液内的荧光素浓度，研究了两种生物源杀虫剂和两种化学源杀虫剂对拟环纹豹蛛肠黏膜通透性的影响。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P＞0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P＜0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P＜0.05),while the difference between control group and blank group was not significantP＞0.05The difference of alkaline phosphatase activities among three groups was not significant(P＞0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP＜0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P＜0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P＞0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后，其各时间点CCK-8法检测值与空白对照无显著差异(P＞0.05)，成骨细胞均在第5天达到增殖高峰期；培养7天后，实验组细胞蛋白含量高于对照组及空白组(P＜0.05)，后两者之间则无显著差异P＞0.05碱性磷酸酶活性在三组间均无显著差异(P＞0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解，第4周时更为明显，纳米珍珠层粉较之微米珍珠层粉降解更快，而空白对照组骨洞阴影仍可见，至8周时，则所有组骨洞均己闭合修复，X-ray下已不可见原钻孔痕迹，恢复正常骨质密度；硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快，空白组速度最慢P＜0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快；由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好，无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快，至植入术后24周，实验组骨缺损区接近正常骨密度，对照组骨缺损区密度较低，空白组则呈现骨不连状态；骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P＜0.05，24周实验组的骨密度值与术前所测得的正常值无显著性差异P＞0.05动物取材大体所见均显示组织相容性良好，骨组织逐渐由植入材料两端向中央生长；常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组，至术后24周，实验组骨髓腔与材料已呈相交通状；硬组织磨片荧光显微镜下观察，两组材料在术后8周处于骨生长最快速时期，16周时速度开始减慢，术后4、8、16周时实验组的新骨生长速度均较对照组的快

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。