荧光性

Both of them have higher fluorescence intensity than coumarin, and they can response to trace TNT, though not very obviously.

这两种物质的荧光强度比香豆素有很大的增强，当和TNT作用时，有一定的荧光响应性。

Compared to NMR techniques, the fluorometric analysis method revealed that there are not only obvious interactions of quinoline or its derivates and cucurbit [ 7 ] uril, but also interactions of quinoline or its derivates and cucurbit[6]uril or cucurbit[8]uril which can not be observed by using ^1H NMR technique.

与核磁共振技术比较，荧光光谱分析方法具有高灵敏性的特点，不仅可观察到喹啉及其衍生物与七元瓜环相互作用而引起的荧光性质变化；还能观察到它们与六元瓜环或八元瓜环相互作用所产生的变化，这是通常的NMR方法所不能实现的。

Methods］ A total RNA from 10 cases withovarian papillary serous cystadenocarcinoma and normal ovarian tissues were extracted by a single step method of gene chip. The cDNA retro?鄄transcribed from equal quantity mRNA derived from 10 cases with ovarian papillary serous cystadenocarcinoma and normal ovarian tissues. The cDNA strand was labeled with fluorescent Cy5 and Cy3(normal ovarian epithelial cells) as probes.

方法］ 采用基因芯片技术，按一步法分别抽提10例卵巢浆液性乳头状囊腺癌患者的癌组织和正常卵巢组织的总RNA并纯化mRNA；逆转录合成以Cy5荧光标记卵巢癌细胞和Cy3荧光标记正常卵巢上皮细胞的cDNA作为探针，在含有8 464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

A novel fluorescent probe, which is both pH-selective and pH-sensitive for the two extreme pH ranges of 1.8-3.4 and 11.6-13.3, was designed by assembling several electronegative atoms in the different positions of a highly conjugated molecule.

通过构建喹啉酮荧光体，将多个电负性氮原子设置于同一个共轭分子的不同电子环境中，制作了对极端pH变化具有灵敏响应的新型荧光探针。

Based on the study of spectrum, the components and technology requirement of time-resolved fluorometer were presented And a concrete method to actualize the system of pulsed Xenon lamp and the assemblage of filter setting was developed Sound clinical evaluation project was designed At last, clinical evaluation of the time-resolved fluorometer was conducted.

在光谱研究的基础上，讨论了时间分辨荧光免疫分析测试仪的各个组成部分及其技术要求，提出了脉冲氙灯和聚光滤光系统的具体实现方法，制定了合理的临床评价方案，并对制成的测试仪样机进行了临床应用性能实验。将我们研制的时间分辨荧光免疫分析仪与国外先进同类型仪器进行一系列对比测试实验后，经相关回归分析所得出的相关系数都在99.5％以上，显著性检验小于0.01。

The company's main products: color detection instruments: Glossiness Miriam, Miriam color spectrophotometry, standard light source for color light box, transmissioní°Miriam, Miriam orange peel, fresh young tester; surface coating physical properties testing instruments: Coating thickness, wet film thickness, temperature tracker, surface humidity instrument, dewpoint meter, roughness instrument, electric spark pinhole leak detectors; Detect mechanical properties of surface coating equipment: zoned grid, and pencil hardness tester , impact tester, hardness testing sticks, Latvia Law / hydraulic adhesion tester, abrasion tester, cylinder / cone bending tester, cups sudden device, surface tension instruments, scratch pen, indentation hardness tester, etc.; paint coating equipment dedicated category: fineness board, drying time recorder, wet adhesion tester, paint ohmmeter, PH, the coating device, mixer, bead mill, mixer, spray gun, putty detector Miriam, white tester, iron cobalt colorimeter, moisture titrator; viscosity-type equipment: Viscosity Cup, density cup, bubble viscosity tube viscometer, infiltration cup rheometer, viscosity units conversion meter; laboratory standard consumables: Mitsubishi pencil tests, 3M adhesive tape test, RCA tape, rubber, the hiding power test paper for color tubes, microscopes dedicated LED ring light source, transfer ink knife and ink scraper, brush, Color Card, gray cards, zirconia beads, glass beads, pieces of cement mortar, the standard of fly ash, the standard viscosity fluid, stopwatch, chemical industry-standard compilation of manuals, paint paint testing manual; test standard Material: tin plate, steel plate, aluminum sheet, asbestos-cement pressure plate, Eterpan, glass, maple panels, MDF, copper film, polyester film, copper; measuring meter and optical instruments: digital calipers, micrometer, dial gauge a high scale, depth gauge, thickness gauge, diameter gauge, gauge block, magnifiers, microscopes, projectors, video measuring instrument; environmental test equipment: oven, constant temperature and humidity box, hot and cold circulating water bath, salt spray test chamber, high temperature resistance furnace, fluorescent UV aging chamber, xenon lamp aging chamber; electronics equipment: infrared thermometer, multimeter, clamp sheet, thermal imager, temperature and humidity meter; anti-static instruments: static tester, surface resistivity device, flat-panel monitor, anti-static footwear tester, wrist strap tester, body Tester; other types of instruments and equipment: torque tester, temperature and humidity recorder, push pull, the vibrometer, speed instrument, analytical balance, industrial Scale Units, dust particle counter, X-ray fluorescence coating thickness measurement and elemental composition analyzer, and so on.

公司主营产品：外观颜色检测仪器：光泽度仪、分光色差仪、标准光源对色灯箱、透射雾影仪、桔皮仪、鲜映性测试仪；表面涂层物理性能检测仪器：涂层测厚仪、湿膜测厚仪、炉温跟踪仪、表面湿度仪、露点仪、粗糙度仪、电火花针孔检漏仪；表面涂层机械性能检测仪器设备：划格器、铅笔硬度计、冲击试验仪、硬度试验棒、拉脱法/液压式附着力试验仪、耐磨耗试验机、圆柱/圆锥弯曲试验仪、杯突仪、表面张力仪、划痕笔、压痕硬度计等；涂料油漆类专用仪器设备：细度板、干燥时间记录仪、湿附着力耐擦洗试验仪、油漆电阻计、PH计、涂膜器、混合机、珠磨机、搅拌机、喷枪、腻子测定仪仪、白度仪、铁钴比色计、水份滴定仪；粘度类仪器设备：粘度杯、密度杯、气泡粘度管、粘度计、渗透杯、流变仪、粘度单位换算计；实验室标准消耗品：三菱测试铅笔、3M测试胶带、RCA纸带、橡皮条、遮盖力测试纸、对色灯管、显微镜专用LED环型光源、调墨刀、墨铲、狼毫刷、色卡、灰卡、锆珠、玻璃珠、水泥砂浆块、标准粉煤灰、标准粘度液、秒表、化学工业标准汇编手册、涂料油漆测试手册；测试级标准基材：马口铁板、钢板、铝板、石棉水泥加压板、埃特板、玻璃板、枫木面板、中纤板、紫铜片、聚酯膜、铜棒；量具量仪及光学仪器：数显卡尺、千分尺、千分表、高度尺、深度规、厚度规、内径规、量块、放大镜、显微镜、投影仪、影像测量仪；环境测试设备：烘箱、恒温恒湿箱、冷热循环水浴、盐雾试验箱、高温电阻炉、荧光紫外老化试验箱、氙灯老化试验箱；电子类仪器：红外测温仪、万用表、钳形表、热像仪、温湿度仪；防静电仪器：静电测试仪、表面电阻仪、平板监测仪、防静电鞋袜测试仪、手腕带测试仪、人体综合测试仪；其他类仪器设备：扭力测试仪、温湿度记录仪、推拉力计、测振仪、转速仪、分析天平、工业台秤、尘埃粒子计数器、 X-射线荧光涂层测厚和元素成分构成分析仪，等等。

SNP ; gene chip ; molecular probe ; fluorescence polarization ; fluorescence resonance ; mass spectrometry ; bacterial magnetic particle

单核苷酸多态性；基因芯片；分子探针；荧光偏振；荧光共振；质谱；磁性颗粒

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

The sizes of the self-assembly micelles of the amphiphilic block copolymers and the effects werestudied by dynamic light scattering and UV-visible spectrophotometer. The micelle sizes werelargely influenced bythe hydrophobic chain content in the copolymer, the properties of the solvent and the copolymer concentration in the organic phase. The morphology of the micelles was investigated by transmission electron microscopy. Theresults showed that the micelles appeared spheres with inner core and outer shell. The critical association concentrations were determined by pyrene monomer fluorescence probe technology. The degradability of copolymer was studied by the loss of the intrinsic viscosity in the degradation process. The results indicated that the degradation rate of PECL was slower thanthat of PELLA and PEDLLA. The stability of the copolymer micelle dispersion was examined by measuring the critical flocculation concentration, which gradually decreased with increasing the content of thehydrophobic chain in the copolymer. The rheological results showed thatthe viscosity ofthe micelle dispersion with higher content of PEG segments was firstly decreased and then increased with the increasingthe temperature. Otherwise, the variation of viscosity was irregular. The viscosity of the micelle dispersion increased with the increase of the electrolyte (Na 2SO 4) concentrations. Polymer micelles paclitaxel was prepared by self-emulsification/solvent evaporation method and solid dispersion technique with amphiphilic block polymers as the carrier material.

运用动态光散射和紫外分光光度计研究了两亲性嵌段共聚物自组装胶束的粒径及其影响因素，结果表明，自组装胶束的形成机理受制备方式的控制，胶束的粒径随着共聚物相对分子质量或疏水嵌段相对分子质量和有机相中共聚物浓度的增大而增大，随着有机溶剂的水溶性的增强而减小；采用透射电镜观察胶束的形态结构，发现胶束呈具有核壳结构的均匀球形；采用芘荧光探针法测两亲性嵌段共聚物的临界聚集浓度；通过降解过程中共聚物特性粘度的变化研究两亲性共聚物的降解性能，发现与PELLA和PEDLLA的相比，PECL 降解速率较慢；随着共聚物中疏水嵌段含量的增大，纳米分散液的稳定性逐渐下降；PEG含量高的PEDLLA纳米分散液随着温度的升高，纳米分散液的粘度先下降然后增大，反之，没有规律性，且随着电解质Na 2SO 4浓度的增大，体系粘度增大。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。