荧光性

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

Morphology of the EPCs was observed, and the growth curve of the EPCs was investigated. Surface antigens of the EPCs were analyzed by the flow-cytometer. The capability of intaking the acetylated low-density lipoprotein of the EPCs was detected using fluoresencent chemical method. The vasoformative capability and genetic stability of EPCs were cultured in matrigel, and examined by karyotype analysis.

观察培养细胞的形态，测定其生长动力学，采用流式细胞仪及免疫细胞化学方法检测其表型，应用细胞荧光化学法分析EPCs对乙酰化低密度脂蛋白(acetylated low-density lipoprotein， ac-LDL)的摄取功能，Matrigel上培养并检测其形成血管能力，染色体核型分析其遗传稳定性，裸鼠实验、软琼脂实验观察致瘤性。

The involvement of oxidization of peroxid in the antigen-antibody reaction makes the cell membrane permeability enhanced, so the fluorescence-labeled antibody stain can infiltrate into the cell easily and contact the antigen rapidly and diffusely, and then the antigen-antibody crosslinking can be formed efficiently.

利用过氧化氢的氧化作用参与抗原抗体反应，使细胞的膜通透性增强，利于荧光标记抗体染液渗入，从而更快速、广泛地接触抗原，更有效的抗原抗体的嵌合交联。

The large area of grey tone to create a background that covers the whole painting and the thin color with an intense vividness of water-color paintings brings the paintings to life; the use of small-area color as flamboyant as fluorescence in the creation of the subject gives the paintings a fable touch; both implements and sets against the other perfectly well. Each implements and sets against the other with perfection.

画家大面积的使用灰色调营造整个画面的大背景，较薄的油彩带有强烈的水彩画的灵动，让画面气韵横生；荧光般艳丽的色彩小面积被用在主体事物的塑造当中，让画作保持着一种寓意性的格调；两者互为衬托，关系处理的很好。

PAO was purified by Ni-NTA resin under denature condition and dialyzed to recover its native structure. After that, the enzyme activity was analyzed with chemical fluorescent method.

IPTG 诱导表达的重组PAO经Ni-NTA亲和层析纯化和透析复性后，化学荧光法测定酶活性。

Take SYBR Green I as the intercalating fluorescence dye in Real-time PCR,the overexpression of WIPK and SIPK,which were the key genes of MAPK cascades,and the resistant gene PR1 in tobacco treated with copper and magnesium solution were studied.

采用SYBR GreenⅠ作为荧光染料的Real-time PCR方法，明确了铜、镁素能调控烟株体内蛋白激酶级联信号转导途径的关键基因WIPK、SIPK及抗病信号通路末端PR1基因的过量表达，进一步揭示了矿质营养调控烟草对病毒抗病性的分子机制。

HE, Hoechest 33258 fludrescence staining and immunohistochemistry of Bcl-2 protein were used to illustrate the osteocyte apoptosis in femoral neck specimens from 10 cases of normal control and 30 cases of femoral head fractures and traumatic femoral head necroses, which were divided into early, middle and advanced stages.

方法］采用光镜观察、Hoechest33258荧光染色及Bcl-2蛋白免疫组化染色对股骨颈骨折和创伤性股骨头坏死患者共30例和10例正常对照的股骨头标本进行组织学观察、骨细胞凋亡和Bcl-2蛋白检测。

Three days after local cerebral infarction , the amounts of catecholamine-containing varicosities and the concentrations of CA in the varicosities in cortices of both sides were markedly reduced, and an aggregation of a large amount of CA granules which were of different fluorescent feature from tha...

局壮性脑梗塞三天后，双侧大脑皮质内CA膨体的数量与膨体内CA的浓度均显著减少，梗塞灶边缘区可见大量CA颗粒聚集，其荧光特征与正常皮质内的CA颗粒不同；双侧大脑皮质内的去甲肾上腺素含量显著减少，多巴胺含量无明显变化。

Methods Ten mature Wistar rats were divided into normal control group (5 rats) and adenovirus (E1, E3-Deleted and carried math1 and enhanced green fluorescent protein report gene, Ad-Math1-EGFP) scala vestibuli transfer group (5 rats). Right ears of the Ad-Math1-EGFP transfering group rats were deliveried 5μl Ad-Math1-EGFP (physical tite 2.1×10^11v.p./ml) into cochleas through the way of drilling scala vestibuli of cochlear basal turn. As a control, the normal group received nothing to inner ear. In order to estimate functional condition of vestibule and cochlea, the click-evoked potentials on the surface of the cervical dura mater, auditory brain stem response and swimming time were recorded in all rats at 7 days after treatment, and then histologic and morphologic observation were carried out after animals were sacrificed. Results All animals' morphologic observation showed that inner ear hair cells were normal after transfer.

将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluorescence protein， Ad-Math1-EGFP)前庭阶导入组，每组5只，实验组大鼠在右耳通过耳蜗底回前庭阶打孔的方法导入物理滴度为2.1×10^11v.p/ml的上述腺病毒5μl，对照组大鼠不做任何处理。7天后对动物进行颈髓硬膜外短声诱发电位(click-evoked potentials on the surface of the cervical dura mater， CDM-CEP)、听性脑干反应阈值检测和游泳试验，评价前庭和耳蜗功能，然后将动物处死进行组织形态学观察。

The results showed that drought stress resulted in reduction of relative water content, protein content, chlorophyll content, corticoid content, net photosynthetic rate, transpiration rate, Fm, Fv/Fm, Fv/Fo and accumulation of proline content, SOD activity, POD activity, MDA content, water use efficiency.

通过测定不同处理条件下小麦代换系及其亲本叶片的生理、生化指标，结果表明干旱胁迫导致相对含水量、蛋白质含量、叶绿素及类胡萝卜素含量、光合速率、蒸腾速率降低，最大荧光、PSⅡ原初光能转换效率以及PSⅡ潜在活性降低，却导致细胞膜透性增大、SOD和POD活性增强、脯氨酸和丙二醛含量增加、水分利用效率增加。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。