荧光性

By adding some fluorescence group in the reaction,we can utilize the fluorescence signal to accumulate and monitor the whole PCR process,and finally through the standard curve,we could analysis the unknown sample.As this technology has the characteristics of strong peculiar,high sensitivity,fine repetitiveness and accurateness,fast and totally closed raction,so in diagnosing animal virosis,this technology not only can measure the power and distribution of pathogen infection in the organism,and also it still has sensitive,fast,convenient characteristics.

该技术实现了PCR从定性到定量的飞跃，同常规PCR法相比，该方法特异性和可靠性更强，能实现多重反应，且采用了完全闭管检测，不需PCR后处理，有效解决PCR污染问题以及EB对试验人员的伤害；再配以相应的荧光PCR仪，使整个PCR过程实现自动化，加之具有耗时短，操作方便等特点而备受推崇，因而广泛应用于基因表达研究、转基因产品安全检测、单核苷酸多态性研究及病原体检测等领域。

Fluorescent colors: Orange, green, yellow, and green, red, pink, Orange, Blue Material: Paper, cardboard, acid resin, wood, PVC polystyrene, polycarbonate, acrylic resin, coating metals, with coating of polyester, ABS Application: Posters, outdoor signs, painting shift pattern printing method, PVC containers, bags and plexiglass Characteristics: Gloss, hiding power, the stability of the screen, flexibility, outdoor weather, the speed of drying, anti-alcohol / chemical resistance, the pressure convex

荧光颜色：桔红，绿，黄绿，红，粉红，桔黄，蓝基材：纸，卡纸，丁酸脂，木材，PVC 聚苯乙烯，聚碳酸酯，丙烯酸树脂类，涂漆金属，带涂层的聚酯，ABS 应用范围：宣传画，室外标志，移画印花法图案，PVC容器，箱包和有机玻璃特征：光泽，遮盖力，网版稳定性，柔韧性，室外耐候性，干燥速度，抗乙醇／化学品性，压凸

The results showed that ZnSe and ZnS QDs exhibit the wurtzite structure, good uniformity, dispersity and fluorescent characteristics.

结果表明：制得的ZnSe和ZnS量子点均是纤锌矿型结构，且具有较好的尺寸均匀性、分散性及荧光特性。

Firstly,we isolated clinically and induced artificially multi-resistant Escherichia coli,detected antibacterials intake by different resistant level strains with CCCP and fluorescence spectro method and ascerained existing active efflux system in this strains; Secondly, construced internal standard of quantitive RT-PCR and detected AcrAB mRNA level of different resistant E.coli with quantitive RT-PCR; Thirdly, prepared AcrA antibody and detected AcrAB protein level of different resistant E.coli; eventually,designed and constructed Taqman probe of AcrAB and detected AcrAB level of different resistant E.coli with fluorescence quantitive PCR.

临床分离及人工诱导大肠杆菌多重耐药株，氰氯苯腙结合荧光分光法检测不同耐药水平菌株对抗菌药物的摄入，确证主动外排系统的存在；构建了定量RT-PCR的内标准DNA，通过定量RT-PCR检测动物源性大肠杆菌AcrAB mRNA的水平；制备AcrA抗体，采用Western-Blotting检测不同耐药株AcrAB蛋白的表达水平；设计和构建AcrA、AcrB的Taqman探针，检测不同耐药菌株AcrAB的水平。

Four anthryl lower aliphatic amine derivatives were synthesized, and DNA binding properties of these compounds were investigated by fluorescence and absorption spectra.

蒽的低级脂肪胺衍生物水溶性很好，不宜固定于PVC膜中，但它们可与核酸分子相互作用，其荧光光谱和吸收光谱发生明显变化。对于不同脂肪胺的衍生物，其作用结果不同。

Use tertiary system method to screen and optimize JLNCAO substrate, and use heat and dry under atmospheric pressure method, ignition residue method, The Casparian law of trace nitrogen, acid...

运用三级系统筛选法对菌草培养基进行筛选优化；用常压加热干燥法、灼烧法、微量凯氏定氮法、酸性洗涤剂法、氨基酸自动分析仪、原子荧光光谱和原子吸收光谱、乙酰丙酮法和热水提取多糖法分别对菌草香菇和木屑香菇的水分、灰分、蛋白质、纤维素、氨基酸、重金属、甲醛、香菇水溶性多糖等的含量进行比较研究。

These excellent properties of 9E-BHVC all mentioned above imply that it would be a true two-photon nucleic acid fluorescent probe if it can specially recognize the DNA in complex cellulous environment.So this dissertation has investigated the ability of 9E-BHVC on the cell nucleus imaging detaily.

与BMVC相比，9E-BHVC表现出作为双光子荧光探针更好的特性，例如具有更大的活性吸收截面和更高的结合常数，且具有更好的水溶性。9E-BHVC这些优良的性质预示着该分子如果能够在复杂的细胞环境中特异性地识别DNA，那么它将是真正意义的双光子核成像探针。

Compared to BMVC,9E-BHVC has many better features needed by a two-photon probe,such as larger two-photon action cross sections,stronger affinity to DNA and better water-solubility.These excellent properties of 9E-BHVC all mentioned above imply that it would be a true two-photon nucleic acid fluorescent probe if it can specially recognize the DNA in complex cellulous environment.So this dissertation has investigated the ability of 9E-BHVC on the cell nucleus imaging detaily.

与BMVC相比，9E-BHVC表现出作为双光子荧光探针更好的特性，例如具有更大的活性吸收截面和更高的结合常数，且具有更好的水溶性。9E-BHVC这些优良的性质预示着该分子如果能够在复杂的细胞环境中特异性地识别DNA，那么它将是真正意义的双光子核成像探针。

In order to study the effect of vascular damage in vivo during PDT, the chicken chorioallantoic membrane tumor model by using stable high GFP-expressing human adenoid cystic carcinoma cell strain is built for the experiment.

为了在活体肿瘤模型上实时监测PDT导致的血管损伤效应，使用稳定高表达绿色荧光蛋白的人涎腺腺样囊性癌细胞株，建立了基于鸡胚尿囊膜的肿瘤模型。

The optimal pH was 8.7. Immunofluorescent and AP-immunocytochemical assays revealed that the HO distributed dispersively in the worm, and located in cytoplasm.

成虫组织切片显示，特异性荧光在虫体内呈散在性分布，细胞碱性磷酸酶免疫染色试验表明该酶位于成虫的细胞质。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。