荧光性

CK, LDH in serum, SOD, MDA in muscular tissue, and Na+-K+-ATPase activity in sarcolemma were measured respectively.

以DPH和N[3P]M为探针，用荧光分光光度计分别测定肌细胞膜脂质流动性和膜蛋白的活动性。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

Objective To evaluate the diagnostic value of fluorescence in-situ hybridization in sex chromosome abnormality.

目的 探讨荧光原位杂交技术在性染色体异常诊断中的价值。

Methods The selected microorganisms were cultivated at 37℃ in culture media that was put different concentration of sodium arsenite,and detected arsenic accumulation in cells by ASA.

各菌株接种在不同浓度含砷培养基中，观察其对砷的耐受性及砷对各菌株的生长影响；利用超声波细胞粉碎机震碎菌体后，用超低温捕获原子吸收分光光度仪检测砷含量，用荧光光谱检测器检测砷形态。

So the present study was taken to compare the differences in the mechanisms of dissipating excess light energy in cucurbit crops and to study the differences in both the ROS metabolism in root system and the photosynthesis among six cucurbit crops with different temperature tolerance growing at different root-temperatures. The main results are as follows: 1、The species specific response of three cucurbit crops with different heat-tolerance: warm-adapted cucumber, heat-tolerant sponge gourd and heat-sensitive figleaf gourd to diurnal changes in air temperature and irradiance intensity were investigated in a typical summer sunny day. Three cucurbit species were compared using parameters in gas exchange, chlorophyll fluorenscence, photon allocation and photosynthetic electron flux.

因此，本试验以温度敏感性不同的瓜类作物为材料，研究了夏季自然条件下不同物种间在耗散过量光能的主要机制差异；探讨了它们对不同根际温度在根系活性氧代谢及地上部光合作用等方面的生理响应差异，所取得的主要结果如下： 1、在夏季晴天条件下，三种温度敏感性不同的瓜类作物：喜温的黄瓜，耐热性的丝瓜与热敏感的黑籽南瓜在气体交换、叶绿素荧光、光能分配及光合电子流对一天中温度与光照环境变化的响应上存在物种间差异和日变化特征。

AMD diagnosis was established by international standard vision chart, Slit lamp microscope, direct or indirect ophthalmoscope examination.

对采用国际标准视力表、裂隙灯显微镜、直接或间接检眼镜检查确诊的42例渗出性AMD患者眼底有活动性病变的50只眼进行HRA+频域OCT检查。50只眼均进行了荧光素眼底血管造影及频域OCT同步检查，其中15只眼还同时进行了FFA、吲哚青绿血管造影和频域OCT同步检查。

METHODS: A series of N-octyl-N'-succinyl chitosan labeled with fluorescein, were incubated with HepG2 liver cancer cells, K562 leukemia cells, A549 human lung cancer cells, and BGC cancer cells respectively and followed by using flow cytometry and enzyme-linked immunosorbent assay to assess the affinity and inhibition of N-octyl-N'-succinyl chitosan for the four kinds of tumor cells already mentioned.

用异硫氰酸荧光素对N-正辛基－N'-琥珀酰基壳聚糖进行标记，再分别与人肝癌细胞(HepG2)、人白血病细胞(K562)、人非小细胞肺癌细胞(A549)及人胃癌细胞共培养，通过流式细胞仪及酶联免疫检测仪测定N-正辛基－N'-琥珀酰基壳聚糖对肿瘤细胞的亲和性及抑制力。

Results Same as hyaline cartilage, the calcified cartilage was stained light flavo-green and weak double refraction by the Picrosirius-polarization method. In the immunohistochemistry analysis of collagen Ⅱ protein, the calcified cartilage was stained strong green fluorescence and the collagen protein parallelled in joint surface near the tidemark. The content of collagen Ⅱ protein was (20.16%±0.96%) of tissue dry weight by amino acid analysis, and that of the hydroxyapatite was (65.09%±2.31%) of the inorganic composition in calcified cartilage.

结果 天狼猩红染色见钙化软骨层与透明软骨层一样黄绿色淡染、弱双折光性；Ⅱ型胶原蛋白免疫组织化学分析，钙化软骨层呈强绿色荧光，潮线附近的胶原蛋白呈板层平行分布；氨基酸定量分析钙化软骨层Ⅱ型胶原蛋白含量占组织干质量的（20.16%〖KG-*4〗±0.96%）；X-射线衍射定量分析钙化软骨层中的羟基磷灰石含量占无机成分的（65.09%±2.31%）。

Triose phosphate isomerase was prepared and purified from chicken breast muscle. The equilibrium unfolding of TIM induced by guanidine hydrochloride was investigated by following the changes in intrinsic fluorescence, circular dichroism and second-derivative spectroscopy.

从鸡胸肌中纯化出磷酸丙糖异构酶(triose phosphate isomerase， TIM)，通过蛋白质内源荧光，圆二色性，紫外吸收二阶导数光谱等多种研究溶液构象的方法，对TIM被盐酸胍和热变性过程进行了详细的研究。

Fluorescence probe study, surface tension and dye solubilization test indicate the watersoluble calixarene, 5, 11, 17, 23, 29, 35, 41, 47-octosulfonato-49, 50, 51, 52, 53, 54, 55, 56octododecyloxycalix [8] arene (1〓-C〓) acts as"unimolecular"micelle in water.

荧光探针、表面张力及染料增溶实验表明，水溶性杯芳烃5，11，17，23，29，35，41，47-octosulfonato-49，50，51，52，53，54，55，56-octododecyloxycalix[8]arene（1〓-C〓）在水溶液中以单分子胶束的形式存在。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。