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荧光性

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Methods Focal cerebral ischemia and reperfusion model in male adult SD rats was established by occluding the right middle artery with 40 nylon thread blood flow blocked for 90 minutes and then reperfusion for 1 day, 7 days and 14 days.One microliter MK801 (5mmol/L and NBQX (50mmol/L) were respectively injected into the cerebral ischemic area of SD rats under stereodirectional instrument right after ischemia.

以线栓法制作成年SD大鼠局灶性脑缺血再灌注模型(阻塞90min再灌注1d、7d和14d),分别向其大脑皮质缺血区立体定向注射5mmol/L 的MK801和50 mmol/L的NBQX各1μl,用免疫荧光组织化学法检测脑缺血再灌注后成年大鼠早期大脑梗死中心区、梗死周边区和缺血对侧NG2和O4阳性细胞数量。

Complex V shows a one-dimensional 'Z' chain which are linked into two-dimensional lamellar structures by weak π-π stackings. Complex VI shows one-dimensional chiral helical chains which are linked into molecular zipper. Both complexes show strong blue photoluminescent under R.T. Complexes VII and VIII are monometallic complexes of Fe.

由对苯二甲酸形成的化合物Ⅴ是由一维的'Z'型链组成的,链链之间通过π-π弱相互作用形成了二维结构;而由间苯二甲酸形成的化合物Ⅵ是由一维的手性螺旋链组成的,链链之间通过π-π相互作用形成拉链状的二维结构,Ⅴ和Ⅵ在室温下具有强荧光。

Objective Using dual color fluorescent in-situ hybridization to detect sex chromosome mosaicism in human embryos and perform preimplantation gender diagnosis.

目的 应用双色荧光原位杂交技术对人类早期胚胎性染色体嵌合型进行初步分析,并进行单细胞种植前性别检测。

Identification of sex by FISH analysis of a single cleavage cell is accurate, and sex chromosome mosaicism will not affect preimplantation gender diagnosis.

应用荧光原位杂交技术可准确地进行单细胞种植前性别诊断,性染色体嵌合型不会影响胚胎的性别诊断。

The analysis result of IR and fluorescence indicated that Eu3phen had been grafted on polypropylene backbone successfully and that of DSC and WAXS revealed that this functional group act as nucleator to increase the crystallization rate effectively and destroy the integrity of crystalline as well.

IR和荧光分析结果表明该配合物已成功接枝到PP链上。而DSC和WAXS分析结果则表明功能化基因的加入起到类似成核剂的作用,有效提高PP的结晶速率,但同时也破坏了PP晶区的规整性。

Eye examination showed blepharospasm ball conjunctival hyperemia, edema, corneal punctate epithelial shedding, decreased corneal sensitivity, corneal fluorescein staining showed diffuse points, or flake coloring, the pupil to narrow as .24 hours, the symptoms and reduce the growth of new epithelium, 48 hour recovery, when the electro-optic stimulation of ophthalmia after symptom onset, can be partially points, 025% dicaine pain, point anti-inflammatory eye drops, hourly, pre-B anti-corneal infection .

眼部检查可见眼睑痉挛,球结膜充血、水肿、角膜上皮点状脱落,角膜知觉减退,角膜荧光素染色呈弥漫性点状或片状着色,瞳孔缩小。24小时内症状随着新生上皮的生长而减轻,48小时可痊愈,当电光性眼炎的刺激症状出现后,可局部点025%地卡因止疼,点消炎眼药水,每小时一次,预B防角膜感染。

The results reveal that 2 has longer absorption, emission maximum and stronger fluorescence emission than 1 because of its stronger polarity and better planarity.

研究发现:染料2由于具有较强的极性和较好的平面性,从而具有较长的吸收、发射峰和较强的荧光发射。

The CPP32 was activated and the 32-KDa proenzyme was cleaved. The active subunit p17 was detected at 3, 6, 9h of incubation and reached the peak at 6h.

用ICE的特异性荧光底物Ac-YVAD-AMC分析ICE活性,证明ICE在整个过程中均未见升高,与正常细胞组相比无显著性差异。

Use quadruplex nested fluorescent PCR and capillary electrophoresis to analysis the polymorphism type of KG8, SM6, CW4 and CW2 of single lymphocytes.

用四重荧光巢式PCR和毛细管电泳对单个淋巴细胞的KG8、SM6、CW4和CW2四个微卫星位点进行多态性分析。

Methods Sera derived from 226 SLE cases and 183 healthy controls were tested for ANA and all parameters were compared such as sensi- tivity,specificity,accuracy,positive predictive value,negative predictive value,positive likelihood ratio,nega- tive likelihood ratio,result consistency,rank correlation coefficient and kappa of ANA detected by IFA...

方法同时采用IFA法和ELISA法检测血清ANA共409例,其中SLE 226例和健康对照183名,将两种方法检测结果的敏感度、特异度、准确度、阳性预测值、阴性预测值、阳性似然比、阴性似然比、结果符合率、判断一致性κ值及秩相关系数进行比较分析;通过ROC曲线比较两种方法的准确度;结果不一致标本进行可提取性核抗原抗体谱检测分析;同时将IFA法检测不同荧光模型样本分别与ELISA结果进行相关分析。

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