荧光性

Lesion was made by current of 1mA, 210s in the dorsal funiculus at the 10th thoracic vertebral level. Behavioral and histological evaluation, HRP and fast blue retrograde labelling method were employed. The eletrolytic current caused bilateral corticospinal tract injury and incomplete paraplegia in hind limbs, and led to a substantial atrophy of corticospinal motoneurons , serving as a reproducible and practical model for research on SCI.

在大鼠T10胸椎节段脊髓后索插入损毁电极，通电1mA，210s，应用行为学评分、脊髓局部组织学检查、HRP和荧光逆行示踪方法发现，电解损伤造成了双侧皮质脊髓束纤维受损，大鼠双侧后肢肌力下降、肌张力升高、运动功能下降，同时大脑运动皮层处皮质脊髓束胞体发生明显逆行性萎缩，可作为一个稳定、可靠的SCI模型。

Methods On the basis of bilirubin induction and NMDA receptor an-tagonist GAPA administration in guinea pigs. The brain extracellular EAA neurotransmitter was ex-tracted with microdialysis and analysed with HPLC; then, brain tissue ATP was measured by fluores-cence assay and intracellular Ca2 + was measured by fluorescence imaging analysis.

制作胆红素脑病动物模型基础上，在体脑内微透析提取兴奋性氨基酸神经递质，HPLC检测天冬氨酸、谷氨酸、甘氨酸，并给予模型动物NMDA受体拮抗剂GAPA干预，电镜检测脑组织超微结构，荧光检测脑组织ATP与细胞内Ca2+含量。

Methods 45 RA patients, 46 patients with other rheumatic diseases and 20 volunteers were enrolled in the study. The frozen sections from human gena mucous and rat esophagus were respectively used as the substrates. The APF, AKA and RF were determined by indirect immunofluorescence.

为45例RA患者，46例非RA的风湿性疾病患者及20例健康人，分别以人颊黏膜和大鼠食管冰冻切片为底物，用间接免疫荧光法检测APF、AKA及RF。

The data of hui ethnic group were generated through co amplification, genescan, genotyping, and genetic distribution analysis.

每个基因座的引物分别用fam，hex或tamra荧光标记，通过复合扩增、基因扫描、基因分型和遗传多态性分析获得数据。

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目前，医院拥有HA-100前列腺腔内场效消融系统、前列腺电解治疗仪、磁振聚焦治疗系统、SW性功能康复治疗仪、美国CRS电容场治疗系统、螺旋水疗治疗系统、固态源治疗系统、显微通脉技术仪、德国"狼"牌宫腔镜、腹腔镜、美国GE彩超、TCT早期宫颈癌筛选系统、美国COOK导丝、"海扶"聚焦超声系统、美国LEEP、德国生物氧治疗系统、乳腺远红外检测仪、芬兰乳腺钼靶、可视人流系统、中药全身汽疗治疗舱、日本数码阴道镜、心电监护仪、全套生化检测设备、全电脑肛肠治疗系统、美国DNR失眠监测仪、儿童注意力缺陷诊断仪、全电脑荧光微量元素分析仪、JAC-CPT 感觉综合训练仪等一大批国内外最先进的诊疗设备，以及以色列飞顿Ⅱ号美肤工作站、SHR超级冰点脱毛系统、ASTER冰电波除皱系统、ASD可视祛斑治疗仪、光子嫩肤仪、生物电美白仪、聚能震波吸脂仪、意大利微晶祛疤仪、新型半导体激光美容系统、整形美容术前设计系统等一整套韩国先进齐全的医学整形美容设备。

Real-time fluorescence quantitative PCR technique has been widely applied, such as mRNA expression, detections of DNA copy number and determination of mononucleotide polymorphism, as well as in the clinical medicine including accurate quantitative examination of mycobacterium tuberculosis, Type B and Type C hepatitis, AIDS virus, gonococcus, and chlamydia trachomatis.

实时荧光定量PCR的应用范围非常广泛，包括mRNA表达的研究、DNA拷贝数的检测、单核苷酸多态性的测定等，已广泛应用于医学临床，它能对结核分枝杆菌、乙型、丙型肝炎、爱滋病病毒、淋球菌、沙眼衣原体等病原体进行准确的定量检测。

RESULTS: hMSC had good homogeneousness and immunological reaction after implantation.

结果： 第5代呈集落生长的hMSC，均一性好。hMSC可在脑内存活6周以上；随着时间的延长，hMSC在脑内迁移的范围越来越大；间接免疫荧光表明hMSC在大鼠脑内表达人神经元特异性烯醇化酶、神经丝蛋白和胶质纤维酸性蛋白；模型鼠的肢体运动功能有明显改善。

When measuring the water permeability, people used to make the cell in the circumstance of hypotonicity. Our research has contrast the other method to make the cell in the circumstance of hyperosmotic using FluoStar Optima in CHO-V163S-AQP4、FRT-V163S-AQP1 and SMMC-7221-V163S. We has confirmed that the latter is more sensitive and accurate than the former in the process of high throughput screening AQP inhibitor drug on the first time.

在测定细胞水通透性变化时，人们通常采用给细胞造成低渗环境的方法，本研究将给细胞造成高渗环境的方法与之做了对比研究，应用FluoStar Optima荧光仪对CHO-V163S-AQP4、FRT-V163S-AQP1以及SMMC-7221-V163S进行两种方法对比测定，首次证明了，在高通量药物筛选AQP阻断剂过程中，后者较前者灵敏度更高、测定结果更准确。

OBJECTIVE: To establish the research system of green fluorescent protein labeled bone marrow mesenchymal stem cells growing in the bone bio-scaffold with calcium and poor lucency.

目的：针对骨性材料含钙、透光性差的特性，拟建立骨性材料复合绿色荧光蛋白基因标记的骨髓间充质干细胞的研究体系。

This thesis deals with the investigation on the chemical constituents of Dioscorea Opposite Thunb and studys the interactions between 5,7-dihydroxy-4′-methoxy flavanone,-epicatechin and bovine serum albumin using fluorescence, electrochemical method ,UV absorption and NMR spectroscopy. The main contents are as follows: Chapter 1: Study of the chemical constituents in Dioscorea opposita Thunb 1. Two methods were used to methylate the fatty acid in Dioscorea opposita Thunb.

本文在前人的工作基础上对我省道地药材-怀山药饮片中的化学成分进行了系统的研究；同时利用核磁共振方法、电化学分析法、荧光光谱法、紫外光谱法分别研究了两种天然酚性化合物5，7-二羟基-4′-甲氧基二氢黄酮，(--表儿茶素与牛血清白蛋白之间的相互作用，主要内容如下：一。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。