荠菜

ObjectiveTo develop an extraction process of organic acids from Capsella buras-pastoris by ultrasonic extraction.

目的采用超声波提取荠菜中的有机酸。

The sandwiches are Capsella, shallot, sugar, bean paste and other four kinds of oil tomorrow.

其馅心有荠菜、葱油、白糖、明油豆沙等4种。

The present paper on the Capsella bursa-pastoris and the white mustard shape characteristic, the for medicinal purposes value aspect carries on the elaboration.

本论文就荠菜和白芥的形态特征、药用价值方面进行阐述。

ObjectiveTo develop an extraction process of organic acids from Capsella buras-pastoris by ultrasonic extraction.

方法采用单因素实验/正交实验，研究了温度、超声波作用时间、料液比、超声波功率对荠菜中有机酸提取量的影响规律。

A new LOS2 (Genbank accession no.: AY559080) gene was cloned from Capsella bursa-pastoris by rapid amplification of cDNA ends .

本研究在荠菜中克隆了一个新的冷相关基因转录调节因子Cblos2。

Sequence analysis showed that BnCOR14 had higher similarity with cold-regulated proteins from Arabidopsis thaliana and Capsella bursa-pastoris and with the typical motifs of late embryogenesis abundant proteins,implied that BnCOR14 might have similar functions possessed by other COR proteins in increasing freezing tolerance of plants.

序列分析表明BnCOR14与拟南芥及荠菜等COR蛋白具有较高的相似性，且BnCOR14具有典型的LEA蛋白序列特征，表明BnCOR14可能在油菜抵抗冷胁迫的过程中具有重要的作用。

And proved that there is CBF gene expression-regulated pathway and transcriptional cascade leading to the expression of cold-responsive genes under cold stress in Capsella bursa-pastoris, which Cbice53 activate the expression of Cbcbf and Cbcbf activate transcription of Cbcor15b and the CbLos2 had a indirect and important regulatory function in controlling cold-responsive genes expression under low temperature like the same pathway in Arabidopsis thaliana.

证明了在荠菜中存在Cbice53激活Cbcbf的转录，Cbcbf激活Cbcor15b的转录，同时CbLos2对Cbcor15b的转录有间接的正向调控作用的抗冷胁迫基因表达级联机制，即存在与拟南芥相类似的抗冷胁迫的CBF基因表达调控途径。

AY506804 gene from apsella bursa-pastoris by rapid amplification of cDNA ends . The full-length cDNA of Capsella bursa-pastoris ICE1 gene (Cbice53) including complete 5'-UTR and 3'-UTR was 1811bp long, containing a 1476bp open reading frame which encodes a 492 amino acids protein.

本研究在荠菜还得到了一个新的ICE1基因（基因bank登录号：AY506804），该基因（Cbice53）全长cDNA为1811bp，含有一个1476bp的开放阅读框，预测编码一个492个氨基酸的蛋白。

All of these attested that there is CBF gene expression-regulated pathway and transcriptional cascade leading to the expression of cold-responsive genes under cold stress in Capsella bursa-pastoris, which ICE1 activate the expression of CBF by binding its promotor and CBF bind the DRE/CRT element in the promoter of the cold-regulated genes and activate transcription of them.

证明在荠菜中存在ICE1通过与CBF启动子部分相结合激活其表达，进而CBF又通过结合抗冻基因启动子部分带有的顺势式作用元件CRT/DRE从而促进抗冻基因表达，提高植物抗冻性的基因表达调控途径，及其它相关转录因子相互作用的级联机制。

In order to direct the construction of plant germplasms by elucidating the relatives among plants at the level of gene, CYP86MF gene analogues from 11 species of 6 genera in Cuciferae were respectively obtained by PCR strategy using gene specific primers designed from conserved regions of CYP86MF gene reported. Sequence comparisonindicated that the similarities among the genes at nucleotide level were over 80%, and the similarities at amino acid level remained above 70%. The differences between the genes at nucleotide and amino acid level between species were 1.0%~ 5.7% and 2.6%~ 7.3% respectively, while those between genera 5.6%~ 22.5% and 7.3%~ 31.2%, respectively. Phylogenetic analysis showed that Brassica was closely related to Raphanus, followed by Rorippa Scop, Arabidopsis Heynh, Capsella Medic orderly, most distantly related to Orychophrogmus.

为了从分子水平阐明十字花科植物间的亲缘进化关系，给植物种质资源的创建提供理论依据，试验根据课题组已报道的CYP86MF基因编码的氨基酸保守区域设计特异引物，运用PCR技术分别从十字花科6个属11个物种中分离克隆到了CYP86MF基因的同源序列，经比较分析，结果表明：这些同源序列的相似性达80%以上，所推导的氨基酸序列相似性达70%以上，且两者种间差异分别为1.0%~ 5.7%和2.6%~ 7.3%，属间差异分别是5.6%~ 22.5%和7.3%~ 31.2%；由氨基酸序列构建的分子系统树可知，在亲缘进化关系上芸薹属与萝卜属较近，其他依次为蔊菜属、拟南芥属、荠菜属，而与诸葛菜属最远。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。