草地夜蛾

Larva of a migratory American noctuid moth; destroys grasses and small grains.

一种迁移类美洲夜蛾的幼虫；破坏草地和小谷类。

Objective To determine the cytotoxic effect of plperine on SF-21 cell line and to investigate the effect of piperine on the ion channel of insect cells.

目的 检测胡椒碱对草地贪夜蛾SF-21细胞的毒性作用和生长抑制作用，以便进一步研究其对昆虫细胞离子通道的影响。

Objective To clone the Bombus ignitus venom protease gene screened from the expression sequence tags of the bumblebee, Bombus ignitus and express it in Spodoptera frugiperda Sf-9 cell.

目的 克隆表达序列标签中筛选出的红光熊蜂蜂毒蛋白酶基因，在草地夜蛾细胞Sf-9中进行表达。

In addition, baculovirus are noninfectious to vertebrates, and their promoters have been shown to be inactive in mammalian cells. Futhermore, the expression level of some recombinant proteins is up to 1000 mg/L. Finally, recombinant proteins are soluble and antigenically, immunogenically and functionally similar to their authentic counterparts. In this article, we used the transfer vector pBlueBac of AcNPV (Autographa californica Nuclear Polyhedrosis Virus) to construct the recombinant baculovirus AcNPV-GM-CSF by in vivo recombinantion.

本文中，我们首先利用苜蓿夜蛾核型多角体病毒带β-Galactosidase 基因标记的非融合蛋白基因转移载体pBlueBac，通过细胞内同源重组的方式将hGM-CSF基因成功地插入病毒AcNPV的基因组中。hGM-CSF基因编码信号肽和完整的氨基酸序列。hGM-CSF基因在感染重组病毒的草地夜蛾培养细胞Sf9中得到表达，感染后的Sf9细胞培养液能刺激人骨髓细胞在体外形成典型的集落，表达水平可达2.7〓CFU／ml。

Objective To further vertify the bioactivities and mechanism of piperine and to investigate the effect of piperine on the ion channel of insect cytes, the toxicity and lethal effect of piperine against Culex pipiens pallens larvae and Spodoptera frugiperda cell line were investigated under the laboratory conditions.

目的研究胡椒碱对淡色库蚊幼虫和草地贪夜蛾 Sf-21细胞的毒力作用和后致死作用，进一步揭示胡椒碱的作用机制及作用方式，以便进一步研究其对昆虫细胞离子通道的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。