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Methods: Inbred WKAH/Hkm rats were used for this study. Pregnant females were treated with busulfan at embryonic day 11. The embryos were removed at E12 to E21 and stained with alcian blue and alizarin red S. The abnormal changes in the treated embryos' hind limbs were observed with a microscope.

近亲交配的WKAH/Hkm大鼠被用于这项研究,妊娠的雌性大鼠在妊娠11天用白消安处理,妊娠12天到21天的胚胎被移开并用阿辛蓝和水溶性茜素红s处理染色,处理后的胚胎后肢的变化可以在显微镜下观察到。

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Do you know, i need you to come back

你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。