茜素蓝

The results shows, drown or etherization to make mouse death, Alcohol-fixed, defatted with 3% potassium dichromate, vitrification with Potassium hydroxide, coloration of bone with Alizarin Red S, the glycerol-ammonia were respectively coloration of cartilage with Alcian blue, vitrification with glycerol which had different concentrations, were the best conditions for making transparent specimen.

结果表明，水淹或乙醚麻醉处死、酒精固定、3%重铬酸钾脱脂、氢氧化钾透明、茜素红硬骨染色、阿利新蓝软骨染色、甘油-氨水漂白、甘油逐级透明的制作方法，获得的透明标本效果最佳。

Among these strains, Bjerkandera adusta Y5012, Cerrena unicolor Y5002, Funalia trogii Y4997, Trametes suaveolens D8325 and Trametes versicolor Y4946 showed stronger capacity to decolorize the 6 different dyes. The factors related to decolorization of orange G and alizarin red of strain Cerrena unicolor Y5002 were studied by the method of shaking flask.

在40株菌株中，黑管孔菌Bjerkandera adusta Y5012，一色齿毛菌Cerrena unicolor Y5002，硬毛粗盖孔菌Funalia trogii Y4997，香栓孔菌Trametes suaveolens D8325和云芝栓孔菌Trametes versicolor Y4946对刚果红、橙黄G、茜素红、结晶紫、中性红和亚甲基蓝均显示出较强的脱色能力。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

A novel determination method of trace chromium in waste water by dual-wavelength spectrophotometry was established based on the fact that chromium can react with alizarin blue S to form red complex in H2SO4 medium.

在H2SO4介质中，铬与茜素蓝S形成红色产物，据此建立了双波长分光光度法测定废水中痕量铬的新方法。

Fig.2 Renal arterioles of 4-week-old SHR,stained by acid alizarine blue×200Bar=50μm

图2 4周SHR肾内小动脉，酸性茜素蓝染色×200

Fig.5 Renal arterioles of 16-week-old WKY,stained by acid alizarine blue×200Bar=50μm

图5 16周WKY肾内小动脉，酸性茜素蓝染色×200

Fig.4 Renal arterioles of 16-week-old SHR,stained by acid alizarine blue×200Bar=50μm

16周SHR肾内小动脉，酸性茜素蓝染色×200

Fig.5 Renal arterioles of 16-week-old WKY,stained by acid alizarine blue×200Bar=50μm

16周WKY肾内小动脉，酸性茜素蓝染色×200

The coordination reactions of Cu and Ni with acid alizarine blue B in the presence of cetyltrimethylammonium bromide micelle were investigated using the microsurface adsorptionspectral correction technique. The aggregation of AABB on CTAB followed the Langmuir isothermal adsorption law. The enrichment of AABB on CTAB sensitized the complexation between Cu or Niand AABB.

应用微相吸附-光谱修正技术，研究了Cu、Ni与酸性茜素蓝B在溴化十六烷基三甲铵胶束上的配位反应，分析了表面活性剂在配位反应中的增效机理，表征了二元和三元结合物的性质。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。