茜素红

Growth characteristcis of primary and passage cells obtained from human fetal calvaria were observed with an inverted microscope.

用碱性磷酸酶染色、钙结节茜素红染色等方法对所获得的成骨细胞进行鉴定。

After the culture under the mineralization fluid condition in about 1 wk, the compact circular group appeared in the intercellular space, simultaneously cell multiplication ability reduced compared with the conventional culture; The sodium alizarinsulfonate dyeing showed the compact and circular lightproof briquetting brown laminated shape in the mineralizd intercellular spac, I collagen immunity group dyeing was strongly positive in the coloring region.

在矿化液条件下培养1周左右，细胞间出现了致密圆形团，同时细胞的增殖能力较常规培养有所降低；茜素红染色结果显示矿化细胞间出现的致密的、圆形不透光团块呈现片状的棕染，着色区域， I型胶原的免疫组化染色强阳性。

The iliac bone defect moulds were prepared and the compound scaffolds were implanted. The compound scaffolds were taken out after four and eight weeks separately, bone healing status and appearance of scaffolds were observed by eyes, the osteoplast growth status in compound scaffolds were viewed by HE and chinalizarin dyeing separately, the degradation of the compound scaffolds in vivo were observed by SEM.

制作髂骨骨缺损模型，在骨缺损处植入骨支架复合体，植入后4周、8周取材大体观察材料与周围骨愈合情况，HE染色及茜素红染色观察支架复合体内成骨细胞生长及扫描电镜观察支架体内降解情况。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜，用茜素红-台盼蓝染色染色行角膜内皮细胞计数；用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变；并于正常对照组角膜比较。

The osteogenic differentiation markers were determined by para position Nitrobenzene phosphoric acid and Alizarin Red S staining.The expressions of CBFA1 and VEGF mRNA in BMSCs were detected by RT-PCR.

采用改良MTT法测定含药血清对P2代BMSCs增殖的影响；用对硝基苯磷酸法和茜素红S染色定量法对BMSCs分化为成骨细胞的标志物进行测定。

Methods: Inbred WKAH/Hkm rats were used for this study. Pregnant females were treated with busulfan at embryonic day 11. The embryos were removed at E12 to E21 and stained with alcian blue and alizarin red S. The abnormal changes in the treated embryos' hind limbs were observed with a microscope.

近亲交配的WKAH/Hkm大鼠被用于这项研究，妊娠的雌性大鼠在妊娠11天用白消安处理，妊娠12天到21天的胚胎被移开并用阿辛蓝和水溶性茜素红s处理染色，处理后的胚胎后肢的变化可以在显微镜下观察到。

The results show that the speed and adsorbability of alizarin red dye on four TiO2 pillared bentonites are much higher. The adsorption dynamics follows the laws of Bangham and Langmuir.

结果表明，4种TiO2柱撑膨润土吸附染料茜素红的速率和吸附量均较大，其吸附动力学行为均遵循Bangham方程和Lagmuir方程所描述的规律。

Alizarin red S ; bovine serum albumin ; UV spectra

茜素红S ；牛血清蛋白； UV光谱法

Alizarin red has the strongest effect in hydroxyl radical scavenging and protein oxidation protection. Resveratrol exhibit strongest inhibition to lipid peroxidation.

茜素红清除OH的能力及对蛋白质氧化的保护作用最强，白藜芦醇对脂质过氧化的抑制作用最强。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。