茜素红

The results shows, drown or etherization to make mouse death, Alcohol-fixed, defatted with 3% potassium dichromate, vitrification with Potassium hydroxide, coloration of bone with Alizarin Red S, the glycerol-ammonia were respectively coloration of cartilage with Alcian blue, vitrification with glycerol which had different concentrations, were the best conditions for making transparent specimen.

结果表明，水淹或乙醚麻醉处死、酒精固定、3%重铬酸钾脱脂、氢氧化钾透明、茜素红硬骨染色、阿利新蓝软骨染色、甘油-氨水漂白、甘油逐级透明的制作方法，获得的透明标本效果最佳。

In the structure of the complex, two oxygens of phenol in the ligand are bound to Ti to form a five membered cyclic complex of titanocene.

在配合物结构中，茜素红配体中的两个羟基氧均与钛键合，形成五元环状二茂钛配合物，且化合物中还存在两个结晶水

And the matrix secreted by differentiated hMSC began to mineralize after 14 days and increased significantly after 21 days.

用分化培养液培养的人骨髓基质细胞，在14d时开始出现矿化结节，在21d时呈现密集的茜素红染色矿化结节。

Alkaline phosphatase and Von Kossa staining were performed at days 14 and 21, respectively.

第14，21天分别进行碱性磷酸酶和钙结节茜素红染色。

In the biochemical analysis aspect, it is first time to use the simple fluorescence spectrophotometer to determine the protein in human serum by red alizarin as the probe of the RLS. The experiment shows that the system HSA-red alizarin has the maximum intensity of the RLS in Britton-Robison buffer of pH4.10 at maximum wavelength of about 360nm. The method has better linear range within 0.040-10.0ug/mL, determination limit is 0.040ug/mL. Moreover, We have selected three other dyes of chromotrope 2R, acid chrome dark blue and pyrocatechin violet as the probes of the RLS in determining protein in human serum and achieving satisfactory results.

在生化分析方面，作者首次使用简易960型荧光分光光度计，利用茜素红作为共振光散射光谱探针对人血清中的蛋白质含量进行了测定，测定结果显示在pH4.10的Britton-Robinson缓冲溶液，波长360nm处出现最大的共振光散射强度，其线性范围为0.040～10.0μg／mL，检出限为0.040μg／mL；另外，作者还筛选了其他三种可用作蛋白质测定的共振光散射光谱探针的有机染料：变色酸2R、酸性铬深蓝、邻苯二酚紫，并深入研究了测定的适宜条件和干扰情况，将这三种有机染料用于人血清中蛋白质含量的测定，均取得了满意结果。

In 10.5 day mouse embryo, by the induction of notochord, mesenchymal cells of ventromedial somite differentiate into sclerotome, cells migrate towards notochord, forming perinotochord tube around notochord, the cartilage is till invisible, alcian dying is faintly positive.

椎体区域发生骨化后（E16.5），阿利辛兰染色呈阴性，茜素红染色呈阳性，仅有Ihh在椎体边缘有表达，无PTHrP表达；在椎间盘区域，仍然保持阿利辛兰染色阳性，仅有PTHrP的表达，无Ihh表达。

In Clark-Lub's buffer solution of pH=3.6, protein combines with ferric-alizarin red S complex to form a ternary complex, which has a maximum absorption at 567 nm. The influencing factors and the spectral mechanism of the reactive system are studied.

在pH为3.6的Clark-Lub's缓冲溶液中，蛋白质与Fe-茜素红S络合物相互作用生成三元复合物，复合物的最大吸收峰波长λ=567 nm，考察了反应体系的各个影响因素，并对反应机理进行了初步讨论。

The phenotype, calcium tuberculation and the expression of alkaline phosphatase were studied with alizarin red staining and modified Gomori Ca-Co assays respectively.

采用碱性磷酸酶Gomori 钙钴法染色、钙结节茜素红法染色及Ⅰ型胶原免疫组织化学染色方法进行鉴定成骨细胞生物学特性。

Compared to the other carbon and nitrogen sources studied, sucrose and malt extract were found to be the suitable carbon/nitrogen sources. The decolorization efficiency of Cerrena unicolor Y5002 to orange G was similar at different orange G concentration below 500mg/L; however, alizarin red inhibited the decolorization reaction at the concentration of 400mg/L or above 400mg/L.

对一色齿毛菌Cerrena unicolor Y5002的液体培养脱色条件进行了研究，其最适碳源和氮源分别为蔗糖和麦芽浸粉；在不同橙黄G浓度下均获得较高的脱色率，因此浓度为500mg/L的橙黄G未对该菌的脱色能力产生抑制作用，而浓度为400mg/L茜素红则对其脱色作用产生明显抑制。

Keywords Biomarker;Metallothioneins;Resonance light scattering;Alizarin red;Xylenol orange;mercury;Light green SF;cadmium;Graphite Furnace atomic absorption spetrophotometry;environmental risk asse

Article From 南华大学；卫生毒理学硕士论文 2007年度生物标志物；金属硫蛋白；共振光散射；茜素红；二甲酚橙；汞；亮绿SF淡黄；镉；石墨炉原子吸收分光光度法；环境风险评价

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。