茜素红

After calcium induction, the alizarin red staining was performed to test the formation of calcium concentration.

并分别采用茜素红钙盐染色和油红O染色进行鉴定。

MAIN OUTCOME MEASURES: Results of alkaline phosphatase staining and alizarin red staining; Results from quantitative analysis of alkaline phosphatase and osteocalcin expression.

主要观察指标：碱性磷酸酶染色与茜素红染色结果，碱性磷酸酶和骨钙素定量分析结果。

After 20 d calcium induction, jacinth calcium salts could be detected in the cells by alizarin red staining.At day 10 after being adipoinduced, the cells showed positive oil red O staining.

结果：成功获得了稳定表达GFP的ADSCs，GFPADSC经成骨诱导20 d后茜素红染色可见有橘红色钙盐沉积；经成脂诱导10 d后油红O染色阳性。

After 20 d calcium induction, jacinth calcium salts could be detected in the cells by alizarin red staining.At day 10 after being adipo induced, the cells showed positive oil red O staining.

结果：成功获得了稳定表达GFP的ADSCs，GFP ADSC经成骨诱导20 d后茜素红染色可见有橘红色钙盐沉积；经成脂诱导10 d后油红O染色阳性。

The adipoinduced MSCs were detected with oil red O staining. RESULTS: ADSCs, stably expressing GFP, were obtained successfully from the transgenic mice with GFP gene. After 20 d calcium induction, jacinth calcium salts could be detected in the cells by alizarin red staining.

结果：成功获得了稳定表达GFP的ADSCs，GFPADSC经成骨诱导20 d后茜素红染色可见有橘红色钙盐沉积；经成脂诱导10 d后油红O染色阳性。

The result shows that MWNT complex filtering membranes have 13.5% and 42.8% adsorptions for resorcin and alizarin red respectively. And, c-MWNT complex filtering membranes have 33.2% and 80.3% adsorptions for resorcin and alizarin red respectively. Comples filtering membrane have a great affinity for resorcin and alizarin red molecules. The membran been easily renewable, can be used repeatedly.

结果表明：制得的MWNT复合膜分别吸附了13.5%的间苯二酚和42.8%的茜素红；c-MWNT复合膜则分别吸附了33.2%的间苯二酚和80.3%的茜素红，复合膜对间苯二酚和茜素红表现出良好的吸附性，而且制得的复合膜极易再生，可重复使用。

Among these strains, Bjerkandera adusta Y5012, Cerrena unicolor Y5002, Funalia trogii Y4997, Trametes suaveolens D8325 and Trametes versicolor Y4946 showed stronger capacity to decolorize the 6 different dyes. The factors related to decolorization of orange G and alizarin red of strain Cerrena unicolor Y5002 were studied by the method of shaking flask.

在40株菌株中，黑管孔菌Bjerkandera adusta Y5012，一色齿毛菌Cerrena unicolor Y5002，硬毛粗盖孔菌Funalia trogii Y4997，香栓孔菌Trametes suaveolens D8325和云芝栓孔菌Trametes versicolor Y4946对刚果红、橙黄G、茜素红、结晶紫、中性红和亚甲基蓝均显示出较强的脱色能力。

After osteoplastic induction, peripheral blood MSCs had strongly positive reactions for alkaline phosphatase staining, total collagen staining, alizarin red staining.

外周血间充质干细胞成骨诱导后，碱性磷酸酶染色、总胶原染色及茜素红染色均呈强阳性；成脂诱导后油红染色呈阳性，有一定数量的脂滴形成。

In this paper, prepared by the self-assembly of neutral red / 3 mercaptopropionic acid modified gold electrodes, alizarin red / glassy carbon electrode, and so on electrode preparation, influencing factors, and the application of electrochemical properties were studied.

本文应用自组装法制备了中性红/三巯基丙酸修饰金电极、茜素红修饰玻碳电极等，对电极制备、影响因素、电化学性质及应用等进行了研究。

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy（32/120; 26.7%）. The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders（22/120; 18.3%）. Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found（12/120; 10%）. Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters（8.3%）. Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters（4.2%）had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现，在总共搜集的120个病例中，其中因年老所导致的产精力不佳为最多，占38例（31.7%），於镜下可见大量精虫黏附於Sertoli cell的表面，并可见Sertoli cell数量明显增多而Leydig cell明显减少，且其细胞核呈现浓染的现象，而在其副睪中仍可见到成熟精虫蓄留於管腔中，但在其输精管内却只有少量精虫存在；其次为睪丸萎缩，占32例（26.7%），镜下可见中度至重度无造精作用，其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在，但Leydig cell数量明显减少且其细胞质内的脂质也明显减少；睪丸发育不全，占22例（18.3%），於生精小管内只见到精母细胞及少量Sertoli cell存在，不见造精细胞分化，於生精小管间质可见Leydig cell减少并伴随结缔组织增生；副睪结石，占12例（10%），肉眼下可在副睪及输精管前段见到黄白色大小不一的结节，触感坚硬，於镜下可见副睪管扩张并有大量成熟精虫蓄积，严重时可见钙化现象，以Von Kossa及茜素红染色均呈阳性反应；类淀粉沉著症，占10例（8.3%），镜下在睪丸间质及血管周围可见粉红均质样的物质沉积，以刚果红染色成阳性反应；马立克病，占5例（4.2%），镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润；睪丸坏死，占1例（0.8%），镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。