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苯胺蓝

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MAIN OUTCOME MEASURES: The morphology of BMSCs was observed by the inverted phase contrast microscope. Expression of BMSC surface antigens was detected by flow cytometer. Osteogenous potential was assessed by alkaline phosphatase staining. Adipogenic potential was evaluated by Oil red O staining. Chondrogenic potential was assessed by toluidine blue staining.

主要观察指标:倒置相差显微镜下观察骨髓间充质干细胞的生长状态,流式细胞仪检测细胞表面标志物的表达,采用碱性磷酸酶染色鉴定成骨能力,以油红O染色鉴定成脂能力,以甲苯胺蓝染色鉴定成软骨能力。

Subchondral bone depicted high-signal intensity on FSE-T〓WI and STIR in 10 cases.In histopathologic examine, pannus was infiltrating the articular cartilage during 10 to 31 days after injection and monocuclear leukocyte infiltration was found.

关节软骨甲苯胺蓝染色切片显示,注入菌液7天后开始,随化脓性炎症时间的延长,关节软骨的染色逐渐变浅,且以表层及中层的软骨染色变浅为明显,而底层软骨染色深度较表层及中层深。

The interaction ratio and constant were calculated by the voltammetric data.4. Studies on the interaction of phenosafranine, toluidine blue with hyaluronic acid.The interaction of PSF and TB with HA were studied by liner sweep voltammetry. The conditions for the interaction were optimized. The working curve for HA was established under the optimal conditions.

考察了有机染料酚藏花红、甲苯胺蓝与透明质酸的相互作用,优化了结合反应条件,在最佳实验条件下绘制了测定透明质酸的工作曲线并成功用于透明质酸模拟样品的测定,结果令人满意。

RESULTS: HE stained cells were fusiform-shaped with pseudopodia, round or oval nucleus. The cytoplasm was blue after stained with the toluidine blue. The immunohistochemical test revealed that there existed positive expression of type Ⅰ collagen in the cells. In the presence of 10% calf serum, IGF-Ⅰ significantly improved cell proliferative activity in a dose-dependent manner within effective dose range.

结果:苏木精-伊红染色细胞多为梭形,有伪足伸出,细胞核为圆形或椭圆形;甲苯胺蓝染色,胞浆为深蓝色;免疫细胞学方法检测表明纤维环细胞有Ⅰ型胶原表达;在体积分数为0.1的血清条件下,胰岛素样生长因子Ⅰ能提高细胞的增殖活性,并且在有效浓度范围内呈剂量效应关系。

Methods This study contained a total of 16 culture groups, including one group as control and 5 groups treated by 5 drugs (calcium L-threonate, sodium L-threonate, alendronate, 17β-estradiol and calcium gluconate) each at the final concentrations of 10^(-9)mol/L, 10^(-7)mol/L, 10^(-5)mo1/L respectively. After 7 days, eight bone slices of every group were stained with toluidine blue and the areas of resorptive pits were analyzed under light microscope; the concentrations of C-telopeptide of type Ⅰ collagen CT(subscript x or Crosslaps in culture supernatants were measured by ELISA.

制备骨磨片,培养OC,并分别加入高中低浓度10^(-5、10^(-7)、10^(-9)mol/L的L-苏糖酸钙、L-苏糖酸钠、阿仑麟酸钠、17β-雌二醇、葡萄糖酸钙,实验设以上各浓度组及对照组(共16组,每组n=8),甲苯胺蓝染色一光镜观察分析骨片上骨吸收陷窝面积,ELISA测定上清液Ⅰ型胶原C-末端肽(CTx或Crosslaps)浓度。

It suggests that the supraspinous ligaments in the patients with ossification of the ligament flavum also have an obvious tendency of ossification.

结果:胸椎黄韧带骨化症患者棘上韧带内胶原纤维增生、肿胀,出现了大量的纤维软骨细胞,甲苯胺蓝染色呈强异染性。

Sections from frontal lobe, occipital lobe, striatum and hippocampus of normal subjects and sections from hippocampus of AD patients were used in hematoxylin eosin, Lox Fast blue, toluidine blue stains and ulex europaeus agglutinin immunostaining.

应用荆豆凝集素(ulex europaeus agglutinin, UEA)免疫组织化学及甲苯胺蓝等染色观察正常增龄病例额叶、枕叶、壳核、海马及AD患者海马的神经元、毛细血管形态改变,应用图像分析技术测算各部位神经元与毛细血管密度比值并分析其与年龄之间的相关性。

Results: By intravital staining with toluidine blue, smear cytologic examination, vaginoscope, pathologic examination and PCR, the positive rates of HPV were 57.14%, 37.86%, 50.0%, 60.71%and 56.43%, respectively.

结果:甲苯胺蓝试验、刮片、阴道镜、病理组织学检查及PCR技术检测人乳头状瘤病毒感染,其湿疣阳性诊断率分别为:57.14%、37.86%、50.0%、60.71%、56.43%。

METHODS: Twenty pationts with spinal tuberculosis were treated by anterior partial vertebrectomy of the diseased vertebrae, iliac grafting and internal fixation via anterior of posterior approach.

20例脊柱结核患者,施行病灶部分切除、髂骨植骨、前路或后路内固定手术,术后将切除的大块病灶骨用MMA包埋、不脱钙骨切片、甲苯胺蓝染色,观察结核特征性病灶在整块骨中的分布情况。

Toluidine blue staining procedure showed that the neural complex consisted of cerebral ganglion, neural gland and loose connective tissue; the cerebral ganglion was divided into three parts as cerebral cortex, medullary layer and transitional zone; the neural gland was composed of glandular lobules, closely apposing at ventral side of the cerebral ganglion; the neurons and nerve fibers were colored deeply, making a distinct contrast with the background, while neither the neurons nor the nerve fibers were distinctly colored by Golgi-argentraffin staining procedure.

苯胺蓝染色法显示,神经复合体由神经节、神经腺和疏松结缔组织组成;神经节分为皮质部、髓质部和过渡带;神经腺位于神经节背侧面,由腺小叶组成;神经细胞呈深蓝色,神经纤维呈浅蓝色,与背景反差较大。Golgi镀银法显示的结果不理想,神经细胞和神经纤维着色不明显。

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