苯胺蓝

The central part was composed of hypertrophic chondrocytes, for which the number increased with time. The cartilaginous tissue at the time of 2 weeks' cultivation appeared strongly positive staining of collagen type Ⅱ immunohistochemistry and safranine "O", as well as strongly metachromatic to toluidine blue.

其中心为肥大软骨细胞，且随培养时间的延长而增多。2周时，软骨组织甲苯胺蓝染色呈强的红色异染反应，Ⅱ型胶原免疫组织化学染色呈强阳性，番红"O"染色呈强阳性。

METHODS: In the experimental group, ADSCs were cultured with the scaffold and induced by CDMP1(50 μg/L) in basic medium for another 2 weeks. In the control group, SD rat cartilage cells were cultured with elementary nutrient liquid with scaffold for another 2 weeks. Twenty nude mice were imbedded the composite of cells-scaffold of the experimental group in their left armpits and the composites of cells-scaffold of the control group in right armpits. Every ten nude mice were killed at 8 and 12 weeks and were examined by safranine O-fast green and toluidine blue staining.

体外培养的脂肪干细胞复合于支架上，加入诱导液(基础培养液+ 50 μg/L软骨形态发生蛋白1)继续培养2周作为实验组；将SD仔鼠软骨细胞复合于支架上，常规培养2周作为对照组。20只裸小鼠，左侧腋窝皮下均植入实验组细胞-支架复合物，右侧腋窝皮下均植入对照组细胞-支架复合物。8， 12周各处死10只，行甲苯胺蓝和番红O-固绿染色。

A new parallel-epiphyseal cartilaginous tissue was found in the subcutaneous tissue 4 weeks after operation. The tissue was stained with HE, toluidine blue, safranine O and collagenⅡ. A large number of chondrocytes and a cartilage lacuna-like structure were observed under a microscope with no obvious inflammatory reaction around the calcium alginate and polylysine microspheres.

结果 体外培养时微球中骺板细胞生长、增殖情况良好；移植皮下后第4周取材可见外形呈类骺软骨样组织块，行HE、甲苯胺蓝、番红"O"及Ⅱ型胶原等染色，镜下观察可见大量软骨细胞及类软骨陷窝样结构，植入的海藻酸钙微球周围组织无明显炎症反应。

At day 7, 14 and 21 after induction respectively, Toluidine blue staining and immunocytochemical staining were performed to detect differentiation.

在诱导第7，14，21天分别行甲苯胺蓝染色、免疫细胞化学染色检测其分化结果。

Toluidine blue and collagen II stainings were positive in the experimental group and negative in the control and blank groups.

实验组甲苯胺蓝染色和Ⅱ型胶原染色为阳性，对照组和空白组均为阴性。

The electrochemical characteristics of toluidine blue immobilized within self-assembled silica gel were studied in detail.

实验中，详细探讨了甲苯胺蓝在凝胶组装修饰电极中的电化学行为。

In this study, we make a systematic observation on localazation of mast cells in rabbit by toluidine blue staining method.

本研究采用甲苯胺蓝染色法和免疫组织化学方法，对肥大细胞(Mast cell，MC)在家兔体内的分布定位进行了系统观察。

The result was observed 6 weeks later by HE staining,toluidine blue staining and type Ⅱ collagen immunohistochemistry.

2周后植入自体股部肌袋，6周取材，HE、甲苯胺蓝染色、Ⅱ型胶原免疫组化鉴定诱导分化效果。

But the residual cells were survival and secreted metachromatic staining proteoglycan with toluidine blue, which was characteristic cartilage matrix.

但剩余的少量细胞成活，形成类似的软骨陷窝并分泌甲苯胺蓝异染的软骨基质。

A method based on the combination of surface plasmon resonance with cyclic voltammetry with the aid of wavelet transform technique was established for the detection of toluidine blue.

将表面等离子体共振技术与循环伏安法联用，并用小波变换提取实验数据，实现了甲苯胺蓝的定量检测。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。