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ChinaObjective:To study the antitoxic role of vesicular monoamine transporter 2 (VMAT2) in transpgenic Chinese Hamster ovary cell.Methods:With the technology of transgene from PC12 to CHO,MTT reduction assay was used to detect MPP^+ toxic effect on wild type CHO and transgenic CHO.

利用转PC12细胞基因到CHO细胞中形成的转基因CHO细胞(cDNACHO,采用MTT比色法检测1-甲基-4-苯基吡啶离子(MPP^+对CHO细胞野生株(wtCHO和cDNACHO细胞的毒性作用,并观察利血平-VMAT2的特异性阻滞剂对MPP^+毒性作用的影响。

A new collector with chelate-ability,diphenyl α-(3-phenylthioureido) alkanephosphonate,is synthesized,and the collecting performance for cerussite ,calcite and quartz,and the relationship between property and structure are investigated, and the depressor function of pyrogallic acid are also studied.

合成具有螯合性能的新型捕收剂———-α(3-苯基硫脲基)烃基膦酸二苯酯,研究该系列化合物对白铅矿、方解石和石英的捕收能力以及结构和性能的关系,并考查焦性末食子酸的抑制作用。

The results show that the ionic liquid Et〓NHCl-AlCl〓 is an effective catalyst for the dimerization.

研究的结果表明,Et〓NHCl-AlCl〓是二聚反应的高效催化剂,反应无需加热且在无溶剂条件下得到的主要产物为环状二聚物1,1,3-三甲基-3-苯基茚满,反应在80℃、进行5min即可达到转化率100%、茚满的选择性为97.3%。

Chocolate, along with coconut, pecans, and many more, all contain phenyl ethylamine, a chemical that produces the feeling of "being in love." Good place to start, dont you think?

巧克力,随着椰子, pecans ,还有更多的,都含有苯基乙胺,化学品产生的感觉"在爱"的好地方开始,唐的 T你觉得呢?

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2~0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%~80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0~0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法,将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合,建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺:胰酶用pH4.5醋酸缓冲溶液提取后,粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液:0.01mol/LNaoAc-HoAc缓冲液(pH4.5,洗脱缓冲液:0.01mol/LNaoAc-HoAc缓冲液(pH4.5)含0.2~0.35mol/LNaCl分两步洗脱,收集激肽释放酶的洗脱峰;DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液:0.01mol/LTris-HCl缓冲液(pH7.5,洗脱液:0.5mol/LNaCl溶液,一次洗脱,收集胰蛋白酶洗脱峰;最后,亲和层析未吸附液用30%~80%硫酸铵分级盐析处理,沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液:0.01mol/LNaAc-HAc缓冲液(pH4.5含1mol/L(NH_4)_2SO_4,洗脱缓冲液:0.01mol/LNaAc-HAc缓冲液(pH4.5)含0~0.6mol/L(NH_4)_2SO_4,分两步洗脱,收集糜蛋白酶的洗脱峰。

From the result of CV, it can be concluded that azomethine diamine and p-amino-phenyl-9-ethyl- carbazolyl azomethine possess electrochemical activity, and the electrochemical activity arised from the heteroatom of molecules.

我们用循环伏安表征了化合物的电化学性质,从循环伏安的结果来看,偶氮甲碱二胺和对氨基苯基-9-乙基咔唑基偶氮甲碱具有一定的电化学活性,并且这些电化学活性主要来自分子中的杂原子。

The results showed that the yield of phenyl glycidyl ether was 96.8% when the mole rate of sodium phenolate and epichlorohydrin was 1.15:1, the mass ratio of ionic liquid and reactant 2:1, reaction temperature 70℃ and reaction time 2h.

结果表明,在反应温度为70℃,反应时间为2h,反应物苯酚钠与环氧氯丙烷的物质的量比为1.5:1,离子液体与反应物的质量比为2:1时,苯基缩水甘油醚的产率为96.8%。

The value of the Wiener Index for a class of linear phenylene molecular graph is calculated, and the linear recursive formulas is obtained, and then the exact expressions are gained by solving some difference equations of the recursions.

文章给出了一类一般线性聚亚苯基图hQ的Wiener拓扑指标的线性递推公式,通过求解差分方程而得到了它的Wiener拓扑指标的精确表达式。

A value of the Wiener index for a class of linear phenylene molecular graphhM is calculated, and a linear recursive formulas is obtained, and then a exact expressions is gained by solving some difference equations of the recursions.

给出了一类一般线性聚亚苯基图hM的Wiener拓扑指标的递推公式,通过求解差分方程而得到了它的Wiener拓扑指标的精确表达式。

Results showed that the hybrid monomer could photopolymerize effectively and both free radical and cationic polymerization processes were improved. When 2-Hydroxy-2-methyl-1-phenyl-1-propanone (1173) was introduced into hybrid system containing UVI-6976, the final conversion of propenyl ether double bond and polymerization rate increased obviously. The comparison of photopolymerization kinetics between hybrid and blend system was investigated as well.

结果表明,以硫鎓盐为光引发剂时,这三种杂化单体均可以有效进行杂化光聚合反应,丙烯基醚和丙烯酸酯双键都显示出较快的反应速度和较高的最终转化率,同时在简单结构的硫鎓盐(UVI-6976)引发体系中加入自由基引发剂2-羟基-2-甲基-1-苯基-1-丙酮(1173)后,丙烯基醚双键的阳离子聚合速率和最终转化率都有了很大提高。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。