苦杏仁酶

Objective To study the specific cytotoxicity of amygdalin to LoVo cells in antibody directed enzyme prodrug therapy.

目的 探讨一种新的前药－苦杏仁苷在抗体导向酶解前药疗法中对人大肠癌细胞株LoVo的特异性杀伤作用。

The toxicity of amygdalin is low, and it can be activated by anti-CEA McAb-β-glucosidase conjugate effectively to kill targetd cells specifically which may be a new way for colorectal tumor therapy.

抗－CEA单抗－β-葡萄糖苷酶偶联物能特异性激活苦杏仁苷，对LoVo细胞产生靶向杀伤作用，有可能成为一种新的肿瘤治疗方法，降低化疗的毒、副作用。

The enzyme-inactivated and fat-skimmed material and 80% ethanol as the solvent were used in the extraction of amygdalin. The optimum extraction techniques were determined by the orthogonal experiment which was based on the single-factor experiment.

原料经灭酶、脱脂后，确定以80%乙醇溶液为溶剂来提取苦杏仁苷，通过单因素实验确定正交实验方案，进而得出较佳的提取工艺。

Bitter almond contains amygdalin and almond oil, amygdalin can be decomposed to benzaldehyde under acidic condition, benzaldehyde is one of the most widely used flavor in fragrance industry. Almond oil contains high contents of unsaturated fatty acids and VE, it is an advanced edible oil, also can be used in cosmetics materials.

苦杏仁含有2-4%的苦杏仁甙，苦杏仁甙在酶或酸的作用下水解后产生具有强烈的杏仁香气的苯甲醛—即苦杏仁精油的主要成分；另外，苦杏仁含有约50%左右的脂肪油，苦杏仁脂肪油含有大量不饱和脂肪酸以及VE，可直接作为高级食用油或高级化妆品的原料。

Grind fragrant gynocardia, chinese dwarf cherry, almond or peach kernel, extract with supercritical carbon dioxide to remove polyunsaturated fatty acid-rich oil and get functional oil byproducts.

将马蛋果、欧李、杏仁或桃仁粉碎，通过超临界二氧化碳萃取脱除富含多不饱和脂肪酸的油脂，副产功能油脂，萃余物通过食用乙醇萃取得到苦杏仁苷粗品，粗品重结晶得到高纯度苦杏仁苷，苦杏仁苷可以进一步通过苦杏仁酶水解得到苯甲醛。

High purity amygdalin was obtained from apricot kernel by the process of enzyme destruction, defatting, solvents extraction and recrystallization.

苦杏仁经灭酶、脱脂、萃取、重结晶，制得高纯苦杏仁苷。

The exemplary anti-cancer compositions of the present invention may also be used as adjuncts with one or more of the following: a surgical procedure, a laser therapy, a micro-radiation pellet implant, a cryogenic procedure, a chemotherapy, a radiation therapy, a electrodessication/curettage, a laetrile therapy, an immuno-therapy, and an enzyme therapy.

本发明技术抗癌制剂独特之处还在于可以与下列之一或多者结合使用：手术治疗、激光疗法、微放射颗粒植入、冷冻疗法、化学疗法、放射疗法、电烧法/刮除术、苦杏仁苷、免疫疗法和酶替代疗法。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。