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If you're suffering depression, humor can change your state of mind.

如果你正遭受抑郁之苦，幽默可以改变你的心理状态。

Besides rich guest website, spend the network video industry that counts venture capital investment to also want again " tighten pants chatelaine " too hard time.

除了博客网站，重度依赖风险投资的网络视频行业也要"勒紧裤腰带"过苦日子。

Rise high to suffer from the one lever red flag on chinaberry in village mouth only, we often are looked up at.

唯有高高升起在村口苦楝树上的一杆红旗，我们经常仰望。

Sequence analysis showed that the amplified 16S rDNA fragment was closely related to those in the 16Sr Ⅰgroup, with a homology rate of more than 99 %. This fragment had maximum homology, upto 100 %, with those of Ash witches' broom, Epilobium phyllody, and Chinaberry witches' broom phytoplasma strain Jiangxi. Its similarity of 16S rDNA sequence is obviously lower than 97 % as compared with other phytoplasma groups.

结果表明，该片段与16Sr I组中的各植原体同源率均达到99 %以上，其中与16Sr I中的白蜡树丛枝（Ash witches'-broom，AWB，AY566302），柳叶菜变叶（Epilobium phyllody，EP，AY101386）和苦楝丛枝江西株系（Chinaberry witches'-broom，CWB-JX，AY859543）的同源率最高，达到100 %，而与其它组的植原体16S rDNA序列的同源率均低于97 %。

Chinaberry tree samples showing witches' broom were collected in Danzhou, Hainan Province. Using universal primers Rl6mF2/Rl6Mr1 for phytoplasma 16 S rDNA, a fragment of about 1.4 kb was amplified, cloned and sequenced from the DNA samples extracted from the diseased Chinaberry tree.

利用植原体16S rDNA通用引物对Rl6mF2/Rl6mR1，通过PCR技术从表现典型丛枝症状的苦楝植株总DNA扩增到约1.4 kb的特异片段，将此片段克隆后进行序列测定、分析及系统关系树构建。

Chinaberry witches'broom, Ziziphus jujube witches'broom, Mulberry dwarf disease, JWB and Paulownia witches' broom phytoplasmas were identified by comparative studies of PCR amplify of phytoplasmal 16SrDNA、23rDNA and ribosomal protein gene, heteroduplex mobility assay, RFLP of PCR-amplified products (16S rDNA), cloning and sequence analysis of 16SrDNA and rp gene. An efficient procedure for rapid identification and differentiation of phytoplasmas was established, which could identify and differentiate phytoplasma collected from field.

通过对桑萎缩病、枣疯病、酸枣丛枝病、泡桐丛枝病和苦楝丛枝病植原体16SrDNA、23SrDNA和核糖体蛋白基因的PCR扩增、异源双链迁移率分析、16SrDNA PCR产物的限制性片段多态性分析以及植原体16SrDNA和rp基因的克隆和序列分析等比较研究，建立了一种快速确定未知植原体种类和分类地位的分子鉴别与鉴定优化程序；此程序可对田间采集的各种植物植原体样品直接进行快速鉴定和鉴别。

The phytoplasmas diseases were investigated in 30 counties and cities of 10 areas in Yunnan Province. There are 12 phytoplasma diseases, including paulownia witches-broom,chinaberry witches-broom, cactus witches-broom, christmas cactus witches-broom, orange little leaf, petunia flat stem and so on were indentified using PCR with universal primers, which amplified phytoplasma 16SrRNA gene and/or ribosomal protein genes. So the kind of phytoplasma diseases in Yunnnan Province were understood. Identified the characteristic of 16SrRNA and rp gene of phytoplasma and the kind, classification and genitic relativity of these strains using RFLP, clonging and sequence analysis (nearly 20 full-length sequences of phytoplasma 16SrRNA and/or rp gene were submitted in GenBank ); The phytoplasma strains isolated from herbage plants were conserved in storeroom. 9 articles were published in the key journals of China and 1 technique invention patent was applied. The net-page about phytoplasma disease was established. 4 graduate student were bringed up.

对云南省10个地区的30余个县市地区进行了植原体病害的调查采集工作；采用植原体16SrRNA基因通用引物（R16mF2/R16mR1及R16F2/R16R2）和/或植原体核糖体蛋白基因引物（rpF1/R1）对所采集到的病害标样总DNA进行PCR扩增，根据植原体特异扩增条带的出现，共鉴定出泡桐丛枝、苦楝丛枝、仙人掌丛枝、蟹爪兰丛枝、柑桔小叶、矮牵牛扁茎等12种植原体病害，初步明确了云南省植原体病害的种类；通过RFLP技术、克隆及测序技术获得其16SrRNA及部分核糖体蛋白基因的近全长序列（已在GenBank中共登录近20余个相关序列），通过序列比较分析，明确了上述植原体株系中这两个基因的特征并确定了云南省植原体株系的种类、分类地位及遗传相关性；对一些发生在草本植物上的植原体进行了株系保存；在国内核心期刊及一般期刊发表研究论文 9 篇，申请技术发明专利1项；初步建立了有关植原体病害的网页；培养研究生4名。

Commonly used in solid wood furniture, wood with Ash, Northeast elm, willow eucalyptus, camphor wood, linden wood, birch, color wood, teak, beech, cherry, red sandalwood, cedar, yew, pine, oak, yellow pineapple, Juglans mandshurica, Schima superba, rosewood, mahogany, Chinaberry, Chinese toon, Jujube and so on.

常用的实木家具木材有水曲柳、东北榆、柳桉、樟木、椴木、桦木、色木、柚木、山毛榉、樱桃木、紫檀、柏木、红豆杉、红松、柞木、黄菠萝、核桃楸、木荷、花梨木、红木、苦楝、香椿、酸枣等。

A bitter alkaloid extracted from chinchona bark; used in malaria therapy.

从金鸡纳树中提取的较苦的生物碱；用于疟疾治疗。

The determination of the chloropicrin remains in five wheat samples by gas chromatography method was discussed in this paper.

探讨了用气相色谱法测定5种小麦中氯化苦残留量的检测方法。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。