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Three expression vectors containing mutant gfp which were truncated from 3′ termine were constructed in the present paper.The mutation gfp with different length under the control by T 7 promoter were expressed in E. coli BL21 cells.

本文将突变型 gfp3′末端不同程度地截短，构建成表达载体，在大肠杆菌 BL 2 1中表达了不同长度的绿色荧光蛋白，当其生色基因中 65位 Ser点突变成 Thr，则 GFP的激发波由 396nm增至 4 70 nm，不用 UV激发，仅在自然光源下就可看到强烈的绿色荧光，发光强度增加 6倍，若从 3′端截短 12个氨基酸密码，对发光无影响，但截短 75个氨基酸密码，则激发波长降到 360 nm，发光强度减少 12倍。

If somebody is twiddling their thumbs , they are bored and have nothing to do.

若某人「 twiddle their thumbs 」，就表示他们觉得很无聊，无事可做。

If the flexible cables have been untwisted automatically, the wind turbine can be restarted automatically without clearance.

若柔性电缆已被自动解开，风机就会无复位的自动重启。

I have seen roses damasked, red and white, But no such roses see I in her cheeks; And in some perfumes is there more delight Than in the breath that from my mistress reeks.

一三○ 一三○我情妇的眼睛一点不像太阳；珊瑚比她的嘴唇还要红得多：雪若算白，她的胸就暗褐无光，发若是铁丝，她头上铁丝婆娑。

And even things without life giving sound, whether pipe or harp, except they give adistinction in the sounds, how shall it be known what is piped or harped?

就是那有声无气的物，或箫，或琴，若发出来的声音，没有分别，怎能知道所吹所弹的是什么呢。

And even things without life giving sound, whether pipe or harp, except they give a distinction in the sounds, how shall it be known what is piped or harped?

14：7 就是那有声无气的物、或箫、或琴、若发出来的声音、没有分别、怎能知道所吹所弹的是甚麼呢。

And even things without life giving sound, whether pipe or harp, except they give a distinction in the sounds, how shall it be known what is piped or harped?

就是那有声无气的物，或箫，或琴，若发出来的声音，没有分别，怎能知道所吹所弹的是什么呢。

And even things without life giving sound, whether pipe or harp, except they give a distinction in the sounds, how shall it be known what is piped or harped?

就是那有声无气的物，或箫，或琴，若发出来的声音没有分别，怎能知道所吹所弹的是什麼呢？

Even things without life that give sound, whether pipe or harp, except they give a distinction of sounds, how shall it be known what is piped or harped ?

就连那些无灵而发声之物，箫也罢，琴也罢，若分不清声调，怎能知道所吹所弹的是什麼呢？

Even things without life that give sound, whether pipe or harp, except they give a distinction of sounds, how shall it be known what is piped or harped ?

就连那些无灵而发声之物，箫也罢，琴也罢，若分不清声调，怎能知道所吹所弹的是什么呢？

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。