苜蓿

To prove the capability of 042BS to nodulate both soybean and alfalfa, constitutively expressed green fluorescence protein gene (gfp carried by pMP2444) was introduced to 042BS, and the recombinant strain 042BSG was obtained. The reisolates from nodules of the soybean and alfalfa inoculated with 042BSG were observed using the confocal laser-scanning microscope, and the expressions of gfp were detected, respectively.

将其接种保定苜蓿和北引1号大豆，并重新分离出根瘤菌，在激光共聚焦荧光显微镜下检测，可以清楚地观察到标记基因的表达，从而确证了042BS能同时在苜蓿和大豆上结瘤。

Sinorhizobium fredii 042BS was isolated from root nodules of alfalfa from Xinjiang Region. Nodulation experiments showed that both soybean and alfalfa were nodulated by 042BS effectively.

费氏中华根瘤菌042BS分离自新疆的苜蓿根瘤，交叉结瘤试验发现它既可以在苜蓿上又能在大豆上结瘤固氮。16S rDNA PCR-RFLP分析表明，042BS与费氏中华根瘤菌模式菌株USDA205的4种限制性酶切图谱完全一致。

Paper electrophoresis showed that the Onobrychis viciaefolia and Medicago sativa cell line transformed with A〓 synthesized agropine and mannopine, and Onobrychis viciaefolia cell line transformed with LBA9402 Bin19 only synthesized agropine.

红豆草转化系获得再生植株。LBA9402 Bin19转化系可以抗100mg／l卡那霉素。苜蓿A4转化系未能得到再生植株。纸电泳分析表明红豆草A4转化系和苜蓿A〓转化系可以合成农杆碱和甘露碱，而红豆草LBA9402Bin19转化系只能合成农杆碱。

The infection process of Pseudopeziza medicaginis on leaves of Medicago sativa was observed by using of differential interference phase contrast microscope, and scanning and transmission electron microscope.

采用微分干涉相差显微镜、扫描和透射电镜技术系统研究了苜蓿假盘菌Pseudopeziza medicaginis在苜蓿叶片的侵染过程及超微结构特征。

In order to investigate the electrotransformation condition of Sinorhizobium meliloti variable curve was devised by different cell cycles time,resuscitative buffer,electrotransfection buffer,pulsed electric field and resistance.

为了研究苜蓿中华根瘤菌电转化的条件，通过对不同的细胞生长周期、复苏缓冲液、电转缓冲液、电场强度和电阻对电转率影响程度来作出变化曲线，以确定电转化的最优条件，从而提高苜蓿中华根瘤菌电转化率。

Despitethe inevitable contamination from ge alfalfa, the eis disavows thisharm and places the entire burden for preventing contamination onnon-ge farmers, with no protections for food producers, consumersand exporters."if roundup ready alfalfa is permitted to be soldcommercially, the ripple effect would wipe out many organic andnon-ge businesses, from organic seed and forage growers to organicdairy farmers and retailers," said liana hoodes, director, nationalorganic coalition.

尽管基因工程苜蓿有不可避免的污染，eis不承认这种伤害，把全部抵制这种污染的负担强加到非基因工程的作物的农民身上，并对食品的生产者，消费者和出口商不采取任何保护。lianahoodes（director， national organiccoalition）说&如果草甘磷苜蓿允许出售，产生的连锁反应将消灭多种有机物和非基因工程产业，从有机种子，饲料加工到有机奶农及其零售商，无一幸免。&

All additions rapidly decreased silage pH under different DM contents of M.sativa.M.sativa(DM 38.45%) ensiled with lactic acid bacteria+cellulase gave the best results: ammonia nitrogen content was reduced,and most CP was conserved and most lactic acid produced.

结果表明，不同干物质含量的苜蓿青贮时，乳酸菌+纤维素酶的添加均能使青贮pH值快速下降；苜蓿半干青贮(干物质含量为38.45%)可使青贮氨态氮含量显著降低，并保存有较多的粗蛋白质，生成较多乳酸，其中以添加乳酸菌106 cfu/g+纤维素酶0.05 g/kg的青贮效果最好，其青贮综合评定得分最高，达93分。

The results show that 0.7% and 1.0% NaCl solutions are appropriate to identify the salt tolerance of alfalfa during geminating period.

结果表明：用0.7%或1.0%NaCl溶液性鉴定较为合理；0.7%NaCl溶液处理苜蓿种子，其幼苗重和幼苗高可作为苜蓿品种耐盐的鉴定指标。0.7%、1.0%NaCl溶液处理下，通过测定第3天和第5天各品种种子的发芽势，可以鉴定耐盐性大小。

Compared to the low drought-resistance BL-02-329, when subjected to drought stress, the net photosynthetic rate in the leaves of high drought-resistance alfalfa, Longdong, decreased more slowly and chloroplast shape and grana structure were less affected.

相对于抗旱性弱的苜蓿，抗旱性强的苜蓿随干旱胁迫程度的加深，净光合速率下降较慢，叶绿体的外形及基粒结构受到的影响较小。

For some kinds of vegetables and pasturages, the contents of some heavy metals surpass the relevant quality standards, so when some crops were planted on the substrates, the uneatable species should be selected preferably and if some kinds of vegetables and pasturages must be adopted, the output could not be provided to mankind and also not be used in farming, in order to avoid harming peoples'health.5 The expediency evaluation model of pioneer plant on substrates was established and some plants were studied. The results show that the expediency evaluation of pioneer plants were respectively that clover was expedient to substrate 1, juniper, cotton and fescue were expedient to substrate 2 and juniper, annual bluegrass, fescue, cotton and clover were expedient to substrate 3.6 Some indexes to evaluate the capability of the phytoremediation on heavy metals and the comprehension about hyperaccumulator were put forward.

有多种植物的重金属含量超过了相应的国家标准，因此在基质上种植植物时，尽量选择非食用性植物品种，即使种植了蔬菜和牧草等植物，也不应将收获后的产品供人类食用或用于畜牧生产，以免危害人体健康。5建立和研究了复垦基质先锋植物的适宜性评价模型，结果表明，不同基质中适宜的先锋植物分别如下：基质1为苜蓿，基质2为桧柏、棉花和高羊茅，基质3为桧柏、早熟禾、高羊茅、棉花和苜蓿。6探讨了评价重金属元素植物修复能力的指标体系和&超富集植物&的概念。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失