苏木红

The controls were only given 0.2mL corn oil injection simultaneously. The rats were sacrificed after suffering from dyspnea, and the survived rats were sacrificed at narcotism in 1 year after the first 3, 4-benzopyrene toxicosis. Lung, brain, liver, esophagus and stomach of all rates were anatomized in search of tumor.

处理后观察1年，期间出现呼吸困难时处死大鼠，首次处理后1年处死全部仍存活大鼠，解剖大鼠肺、食管、胃肠道、肝及脑等脏器，观察有无肿瘤形成，并对肿块及全部大鼠的肺组织切片进行苏木素伊红(hematoxylin and eosin，HE)染色分析。

Beijing 100034,China(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,Beijing 100034,China)(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,Beijing 100034,China)Abstract : Objective In order to study the effects of ATP on U937 cells. Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used.

应用ATP(0.23g/L)作用于人白血病细胞U937，(1)用白细胞计数器每日计数细胞和计算抑制率；(2)用流式细胞分析仪文献检测 ATP对U937细胞周期改变和凋亡的影响；(3)苏木精-伊红染色和电子显微镜文献检测凋亡小体的形成过程；(4)用苯胺黑染色文献检测凋亡小体的生命本性。

MAIN OUTCOME MEASURES: The seeded cells were observed under inverted microscope; at 1, 2, 3 weeks after seeding, the BMSCs were treated with 4% paraformaldehyde and stained with hematoxylin-eosin; The protein content in seeded cells was determined by bicinchoninic acid assay, and the content of DNA was quantified using Hoechst33258 assay at 5, 10, 14 days after culture.

主要观察指标：倒置显微镜下观察细胞形态；于培养1，2，3周采用40 g/L多聚甲醛固定，常规组织切片，苏木精-伊红染色；在培养5，10，14 d用Hoechs33258荧光法定量测定细胞内DNA含量，BCA法测定蛋白质含量。

Aortic specimens were stained with hematoxylin-eosin and Prussian blue for histological observations.

腹主动脉标本进行苏木精-伊红和普鲁士蓝染色观察组织学改变。

Staining observation: The grafts were removed at 1, 2 and 3 months after transplantation, then prepared into sections, and observed by hematoxylin and eosin, Safranin'O and Masson's trichrome stainings.

染色观察：移植接种后1，2，3月取出移植物，切片，行苏木精-伊红、Safranin'0、Masson's trichrome染色观察。

TheThe Europeans immediately replaced a similar dye, extracted from sappanwood, with this new one, to dye fabric.

欧洲人立刻用这种新染料取代了原来从sappanwood（苏木，别名苏方木、红柴。

METHODS Sterile polyvinyl alcohol sponge discs which were labeled as physiological saline group and sinapine groups were implanted into the subcutaneous part of rat.

方法将聚乙烯醇海绵盘随机分为生理盐水对照组和不同剂量芥子碱组，植入小鼠皮下，构建盘状血管生成模型，观察术后12d的血管生成情况及组织生成情况，以蛋白定量检测试剂盒检测在海绵组织中的可溶性蛋白含量，对海绵冰冻切片行苏木精-伊红染色，应用计算机图像处理分析系统分析海绵内组织生长情况。

At 1, 3, 5, and 7 days, cellular proliferation was detected by methyl thiazolyl tetrazolium assay.

60，80 g/L 载药支架组种植角质形成细胞，分别于4，7，14 d 取材，进行苏木精-伊红染色和扫描电镜观察。

MAIN OUTCOME MEASURES: At 15 days after model preparation, one rat was obtained from each group to underdone morphological observation. At 21 and 30 days after model preparation, 8 rats were killed in each group, hematoxylin-eosin staining and immunohistochemical method were used to detect the expression of the proliferating cell nuclear antigen, and to calculate fibroblast proliferation index.

主要观察指标：于造模后15 d各组取1只大鼠行组织形态学观察；造模后21，30 d，每组各处死8只大鼠，取材后，行苏木精-伊红染色，免疫组织化学检测增殖细胞核抗原的表达，并计算成纤维细胞的增殖指数。

The liver tissues of rats in all groups were stained by hematoxylin-eosin ,reticular fiber staining,and were employed by immunohistochemical response,and then the pathological changes, fibra accrementition and the expression of ILK andα-smooth muscle actin in rat liver tissues were observed.

对2、4、6、8周肝纤维化模型及对照组大鼠肝苏木素-伊红染色、网状纤维染色、免疫组织化学反应，观察肝组织病理变化、纤维增生及ILK和α-SMA的表达情况。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。