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Immunohistochemical method: The CD34 monoclonal antibody was selected as the first antibody, with the immunohistochemical SP procedure, then the pathological slice was displayed by DAB and stained again by hematein. One slice was used as a negative control and reacted with PBS fluid(0.01mmol/L) which was the substitute of anti- CD34.(the kits coming from Boster Biotechnology CO.LTD). The number of the vessels and vascular endothelial cells were displayed by immunohistochemical method.

免疫组化方法:病理组织切片以CD34单克隆抗体作为第一抗体,按照免疫组织化学SP步骤进行,DAB显色,苏木素复染,每组用0.01mmol/l的PBS液替代CD34抗体作阴性对照(试剂购自于武汉博士德公司),应用免疫组化显示血管,对不同时期的血管生成情况及早期的炎性反映情况进行观察。

Determination of total hydroxybenzene, extractive technology by orthogonal design and immuneological effect in way of hemolysin and carbon particle clearance determinations of Caesalpinia Sappan L.were studied for the first time.

本研究中,有关苏木中总酚含量的测定方法、苏木提取工艺的正交优选法以及采用溶血素法和碳粒廓清法对苏木提取物进行的药效学研究,至今未见报道。

In conclusion, three extracts restrained the form of hemolysin antibody, but the result wasn't significant in statistics. However, high, middle and low doses of the watery extract and high dose of sixty-five of ethanol extract of Caesalpinia Sappan L. markedly restrained endodermis phagocytosis function.

结果表明,三种提取物均具有抑制溶血素抗体生成的趋势,但无统计学上的意义;高、中、低剂量的苏木水提取物和高剂量的65%乙醇提取物对网状内皮吞噬功能具有明显的抑制作用,从而进一步证实了苏木的水提取物具有免疫抑制作用。

Sodium arsenite group and sodium arsenate group were made by 300 mg/L per day of sodium arsenite and sodium arsenate respectively. The mice were sacrificed after 10 months for liver function and pathologic examination. The expression of MMp-8, TIMP-2, Col-Ⅰ、Col-Ⅲ mRNA were detected with real time flourescence quantitative PCR. The result was controlled with 18s.

亚砷酸钠组和砷酸钠组给予普通饲料和砷溶液。10个月后处死小鼠,血清进行肝功能检测;苏木素-伊红染色部分肝组织;Trizol-酚-氯仿一步法提取小鼠肝组织总RNA,紫外分光光度法测定总RNA浓度及纯度,实时荧光定量PCR法测肝组织中MMP-8、MMP-2、Col-Ⅰ、Col-Ⅲ mRNA的表达,以18S基因作为质控。

PTAH staining for striated muscle illustrated that 70-80% differentiated MSCs could be stained positively.

体外5-aza诱导分化MSCs后,应用磷钨酸-苏木素作横纹肌染色,发现所诱导的MSCs染色阳性率达70-80%。

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