苏木

Beijing 100034,China(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,Beijing 100034,China)(The School of Oncology,Beijing Institute for Cancer Research,Beijing Medical University,Beijing 100034,China)Abstract : Objective In order to study the effects of ATP on U937 cells. Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used.

应用ATP(0.23g/L)作用于人白血病细胞U937，(1)用白细胞计数器每日计数细胞和计算抑制率；(2)用流式细胞分析仪文献检测 ATP对U937细胞周期改变和凋亡的影响；(3)苏木精-伊红染色和电子显微镜文献检测凋亡小体的形成过程；(4)用苯胺黑染色文献检测凋亡小体的生命本性。

MAIN OUTCOME MEASURES: The seeded cells were observed under inverted microscope; at 1, 2, 3 weeks after seeding, the BMSCs were treated with 4% paraformaldehyde and stained with hematoxylin-eosin; The protein content in seeded cells was determined by bicinchoninic acid assay, and the content of DNA was quantified using Hoechst33258 assay at 5, 10, 14 days after culture.

主要观察指标：倒置显微镜下观察细胞形态；于培养1，2，3周采用40 g/L多聚甲醛固定，常规组织切片，苏木精-伊红染色；在培养5，10，14 d用Hoechs33258荧光法定量测定细胞内DNA含量，BCA法测定蛋白质含量。

Aortic specimens were stained with hematoxylin-eosin and Prussian blue for histological observations.

腹主动脉标本进行苏木精-伊红和普鲁士蓝染色观察组织学改变。

Staining observation: The grafts were removed at 1, 2 and 3 months after transplantation, then prepared into sections, and observed by hematoxylin and eosin, Safranin'O and Masson's trichrome stainings.

染色观察：移植接种后1，2，3月取出移植物，切片，行苏木精-伊红、Safranin'0、Masson's trichrome染色观察。

Result: Ethyl acetate extract of sappanwood can evidently reduce the content of CD4~+ of T cell and the ratio of CD4~+/ CD8~+, moreover have no infection on the content of CD8~+ of T cell.

结果：苏木乙酸乙酯提取物组同空白对照组相比能明显降低CD4~+T细胞含量并降低CD4~+/CD8~+的比值，而对CD8~+T细胞的含量无影响。

Ethyl acetate extract of sappanwood can reduce the content of CD4~+ of T cell and the ratio of CD4~+/ CD8~+ of spleen,possess determinate function on immuno-supression.

苏木乙酸乙酯提取物能降低正常小鼠脾细胞CD4~+T细胞含量并降低CD4~+/CD8~+的比值，具有一定的免疫抑制作用。且无量效关系。

TheThe Europeans immediately replaced a similar dye, extracted from sappanwood, with this new one, to dye fabric.

欧洲人立刻用这种新染料取代了原来从sappanwood（苏木，别名苏方木、红柴。

METHODS Sterile polyvinyl alcohol sponge discs which were labeled as physiological saline group and sinapine groups were implanted into the subcutaneous part of rat.

方法将聚乙烯醇海绵盘随机分为生理盐水对照组和不同剂量芥子碱组，植入小鼠皮下，构建盘状血管生成模型，观察术后12d的血管生成情况及组织生成情况，以蛋白定量检测试剂盒检测在海绵组织中的可溶性蛋白含量，对海绵冰冻切片行苏木精-伊红染色，应用计算机图像处理分析系统分析海绵内组织生长情况。

At 1, 3, 5, and 7 days, cellular proliferation was detected by methyl thiazolyl tetrazolium assay.

60，80 g/L 载药支架组种植角质形成细胞，分别于4，7，14 d 取材，进行苏木精-伊红染色和扫描电镜观察。

MAIN OUTCOME MEASURES: At 15 days after model preparation, one rat was obtained from each group to underdone morphological observation. At 21 and 30 days after model preparation, 8 rats were killed in each group, hematoxylin-eosin staining and immunohistochemical method were used to detect the expression of the proliferating cell nuclear antigen, and to calculate fibroblast proliferation index.

主要观察指标：于造模后15 d各组取1只大鼠行组织形态学观察；造模后21，30 d，每组各处死8只大鼠，取材后，行苏木精-伊红染色，免疫组织化学检测增殖细胞核抗原的表达，并计算成纤维细胞的增殖指数。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。