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The development of Amanita manginiana in the study site, from sexual spores to mature sporocarps, might take more than one year.

在对隐花青鹅膏菌连续两年的种群结构进行了研究中,种群的每一个子实体也均来自不同的克隆。

Fruit of Angeleno plum cultivar were used as material for studying the changes and relationship of the content of anthocyanin, endogenous hormone, PAL and PPO activity in the skin of Angeleno plum fruit.

为了明确李果实花青苷合成的调控因子,以安哥诺李为试材,对果皮花青苷、内源激素和PAL、PPO活性的变化以及内源激素、酶活性与花青苷的相关性进行研究。

The content of anthocyanin is higher in spring and autumn.

春秋两季花青苷的含量较高,而叶绿素含量则是在夏季最高。

In addition,LOX activity Was closely corelated with the change of anthocyanin content.

脂氧合酶的活性也与花青苷含量的变化密切相关。

Change of anthocyanin content was closely correlated with the activities of antioxidant enzymes.

结果表明,花青苷的含量变化与果实抗氧化酶活性密切相关。

Anthocyanin content and activities of related enzymeswere measured in'Ralls'and its budmutation. Effect of bagging on anthocyanin biosynthesis in bud mutation was also studied.

以'国光'苹果及其红色芽变为试材,测定了果实发育期间的花青苷含量及其相关酶活性,并研究了套袋对芽变花青苷合成的影响。

The results showed:①Anthocyanin content in mature fruits was 132170 U·g-1FW in bud mutation which was very significantly higher than that in'Ralls'(49140 U·g-1FW);②Activities of PAL and UFGT were almost the same between the two materials, but CH I and DFR activitieswere significantly high in bud mutation, which were related to the increase of anthocyanin levels in the mutant;③Bagging treatment inhibited anthocyanin synthesis of bud mutation. But anthocyanin content had significantly increased after removing the bags. Activities of CHI and UFGT increased rap idly and were much higher than those in the control after the bag removal. The increase was consistentwith the imp rovement of anthocyanin levels.

结果显示:①在果实发育期间,芽变果皮的花青苷含量明显高于'国光',尤其是成熟期芽变果皮花青苷含量为132170 U·g-1FW,而'国光'仅为49140 U·g-1FW;②在果实发育期间,两个品种间PAL 和UFGT的酶活性无明显差异,但芽变的CHI和DFR酶活性明显高于'国光',表明芽变花青苷合成能力的提高与CHI和DFR酶活性高有关;③套袋抑制芽变果皮花青苷的合成,但解袋后花青苷的含量极显著升高,解袋后4种酶的变化趋势差异较大, CHI和UFGT活性均迅速升高,明显高于对照,这与解袋后花青苷迅速合成相吻合。

When the fruits matured anthocyanin content of black currant was highest, that of red currant was subsequence, that of white currant was extrme low.

果实成熟时,黑穗醋栗花青苷含量最高,红穗醋栗花青苷含量次之,白穗醋栗花青苷含量极低。

The characteristic of anthocyanin accumulation and related factors on anthocyanin synthesis of three currant varieties fruits were analysed. The results were as follow:Great quantities of anthocyanin were accumulaed in black and red currant fruits during the late stage of development, but white currant fruits synthetized anthocyanin during the early period of development.

以三种穗醋栗为试材,对果实中花青苷积累及其相关因素进行了分析,研究结果如下:黑穗醋栗和红穗醋栗在果实发育后期大量积累花青苷,而白穗醋栗仅在果实发育初期合成花青苷。

The result would be used in tobacco breeding.4 SRAP technology is used to study the genetic differences among different types of tobacco, including flue-cured tobacco, oriental tobacco, maryland tobacco, burley tobacco, and air/sun cured tobacco, five pairs of primer combinations which can amplify characteristic bands has been screened. Among the five combinations, two of them (SF25*SR2 and SF3*SR26) can distinguish air-cured tobacco including maryland tobacco and Burley tobacco from the other cultivated forms; SF20*SR14 can distinguish sun cured tobacco including oriental tobacco and sun-cured tobacco; SF16*SR14 can distinguish flue-cured tobacco and air-cured tobacco, and SF22*SR14 can distinguish DBJ599 with specisl aroma and the other flue-cured tobacco.5 8 varieties , which represent the different types of tobacco, are divided into 3 groups. I: G-28, II:DBJ599、MD40、By21, III: Basma、Sumsun、XNS、QCT. The cluster analysis result es consistant with experience evatuation.6 Anorthogonal design was used to optimize a ILP-PCR system with 5 factors (DNA, Mg2+, dNTP, primer and Taq polymerase) at 4 levels.The result showed the optimized ILP-PCR system for Nicotiana tabacum L. was: 2uL10×PCRbuffer, 20ng template DNA, Mg2+ 2.0mmol/L, dNTP 150umol/L, primer 0.3umol/L, Taq DNA polymerase 1 U in a total of 20uL reactionsolution.

研究结果可作为烤烟与香料烟类型间的杂种鉴定利用的实验与理论依据。4、利用SRAP标记技术,研究和分析了烤烟、晒烟、晾烟、白肋烟、香料烟等不同栽培类型间的基因型差异,初步筛选出了能够在分子水平上反映不同烟草类型差异的特征性标记带:晾烟MD40、Ky17、Burley21、TN90基因型共有的特征带有两对引物组合,分别为SF25*SR2和SF3*SR26;晒烟小牛舌、青梗、小花青和香料烟Sumsun、komotini Basma、Xanthi Basma共有的特征带引物组合为SF20*SR14;NC89、K326、G-28、大白筋和MD40、Ky17、Burley21、TN90共有的特征带引物组合为SF16*SR14,烤烟中大白筋599的特征带引物组合SF22*SR14.5、利用61对SRAP引物组合,研究了普通烟草不同类型间的亲缘关系,将普通烟草类型间8个不同供试材料划分为3类别。I:烤烟类型G-28II:白肋烟类型(MD40、By21、大白筋599III:香料烟类型(Basma、Sumsun、小牛舌、千层塔)。

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