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A unisexual reproductive structure of gymnospermous plants such as conifers and cycads, typically consisting of a central axis around which there are scaly, overlapping, spirally arranged sporophylls that develop pollen-bearing sacs or naked ''.

球果单性裸子植物的生殖器,例如针叶树和铁树目植物,一般由覆满鳞片和褶皱的中心轴构成,长螺旋状排列的孢子叶,孢子叶最终成为花粉囊或裸露的孢子或种子

Both the pollen grains and the gynoecia are active.

花粉粒和雌蕊都具有活力。

Therefore,foliar spraying ofboron was the main measure during flowering period so as to stimulate thegermination and growth of pollen tube,regulate endogenous hormone balance ofovary and promote gynospore formation and,during the phase of young embryodevelopment,spraying of relevant plant growth regulators and potassiumsupplement were essential to stimulating the increasing of endogenous GA1/3 andiPAs,raising the key nutritious substance content in ovary and promoting youngembryo development.

因而花期调节以喷B为主,以便促进花粉管萌发生长,调节子房内源激素平衡,促进大孢子形成;幼胚发育期以植物生长调节剂喷布和K素补充为主,以便启动子房内源GA1/3、iPAS等上升,增加还原糖、淀粉、蛋白质积累和K素吸收,促进幼胚发育。

Corona lobes inserted at base of gynostegium; pollinia pendulous.

副花冠裂片合蕊冠的着生的在基部;花粉块下垂。

Corona lobes inserted on backs of anthers or gynostegium; pollinia erect or horizontal.

花药或合蕊冠,副花冠裂片着生于回来;花粉块直立或者水平。

The effect of pollinators on the plant sex allocation, may be a new fied to study the evolution of hermaphroditic plants.

传粉者对花粉和胚珠配置的潜在影响,可能为研究两性花的进化打开一条新路。

Objective To construct humulus scandens pollen major allergen DNA vaccine pcDNA-LC2 and evaluate its immunogenicity.

目的 构建葎草花粉主要变应原核酸疫苗pcDNA-LC2并鉴定其免疫原性。

Experimental results show that the proposed method can be used to prepare high titer and specific rabbit anti-Humulus pollen protein polyclonal antibody. It has similar ability in determining the composition of Humulus pollen allergic protein compared with sera of patients. Therefore, it can be used to immunoscreen cDNA library.

制备的兔抗血清具有较高效价和较好的特异性,应用该血清筛选出的葎草花粉致敏蛋白组分与患者血清筛选的组分相似,因此可进一步应用于初筛cDNA表达文库。

The specificity of polyclonal antibody was detected with Western blotting, which applied rabbit antiserum and sera of known Humulus pollen allergic patients.

同时对葎草花粉过敏性哮喘患者血清进行Western blotting检测。

An Jiaotong University, Xi?an 710004, ChinaABSTRACT: Objective To construct humulus scandens pollen major allergen DNA vaccine pcDNA-LC2 and evaluate its immunogenicity.

目的 构建葎草花粉主要变应原核酸疫苗pcDNA-LC2并鉴定其免疫原性。

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