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It was observed that cellular contents, including chromatins and organelles,migrated in PMCs through cytoplasmic channels,mainly at the stage of synizesis in meiotic prophase Ⅰ.

观察发现,处于凝线期的花粉母细胞,其内含物,包括细胞器和染色质,主要通过胞质通道向相邻细胞发生转移。

Using RAPD fingerprinting, we detected changes in PMCs'genomic DNA in Lilium davidii during prophase Ⅰ. The electrophoresis results revealed a specific polymorphism at synizesis stage, but not at other stages or in somatic cells.

采用RAPD指纹技术对兰州百合减数分裂前期Ⅰ中DNA结构变化的研究表明,在凝线期的花粉母细胞中存在着特异的DNA电泳谱带,在其它时期及体细胞对照中均没有。

With DGD method, the electron microscopy observation has provided new information on the process of chromatin migration. That is: Firstly, the nucleus moved toward cell wall and a picture characteristic of synizesis stage of meiosis appeared; Secdonly, the transmigration of chromatin occured through CC, and a more extensive region constituted of nuclear skeleton left behind chromatin, which was named as"clear spaces" under light microscopy; Thirdly, at the late stage of synizesis, most of chromatin had gone into adjacent cells through CC and fused into a whole, and the nuclear skeleton left in the former cell gradually mixed with cytoskeleton in cytoplasm.

DGD—包埋去包埋电镜观察表明:百合花粉母细胞中有类似核纤层的结构存在;在染色质穿壁运动过程中,首先是细胞核向细胞壁靠拢,并可留下瞬间的运行轨迹——细胞核后方出现只有细胞骨架而少有细胞器的区域;其后是染色质开始穿壁,在穿壁染色质后方细胞核内出现无染色质仅有密集的核骨架的区域,到了染色质穿壁后期,大部分核物质都已穿至相邻细胞,并彼此融合,而残余在原穿出细胞中的核骨架己逐渐与原细胞的胞质骨架融为一体。

It is deduced that the abnormal development of tapetum and the abnormal distribution of Ca(superscript 2+) are related to the pollen abortion.

推测绒毡层细胞结构发育的异常和Ca(上标 2+)的异常分布可能与花粉的败育有关。

It was speculated that the sterility of the RNMS line was related to the delay of tapetum cells breaking down.

因此,四川隐性核不育是绒毡层降解延迟导致小孢子解体而发生的无花粉雄性不育。

The BcPW1 gene can act on the signal transmission between a tapetum and pollen during the development process of a cell wall.

BcPW1基因可能作用于细胞壁发育过程中绒毡层与花粉的信号传递过程。

They were expressed specifically in pollen, tapetum and ripped anther, showing a high level of S haplotype specific sequence polymorphism, similar to that of the S-RNases.

通过分子生物学方法和杂交授粉试验证明所分离的F-box基因是花粉自交不亲和基因,但目前尚未分离出该类基因相应的表达蛋白。

During male and female reproduction organ developments, CBKs were expressed obviously in temporal and spatial difference manners, especially in pollen development, tapetum cell development and degeneration and ovule development.

这种差异性突出体现在花粉细胞的发育、绒毡层细胞的发育和凋亡以及胚珠的发育等过程中。

The tapetum cells oftrisomic 7 and 10 showed abnormal phenomenon at meiosis,but trisomic 7 recovered normality at other periods.

而10号三体的绒毡层虽在四分体时期表现正常,但在小孢子时期又表现了异常,并且花粉全部败育。

In succession, the middle layer of anther wall had the strongest Ca^2+ fluorescence intensity, and the tapetum took second place during the meiosis of microspore mother cell.

小孢子内Ca^2+分布不均匀,细胞质中的Ca^2+荧光较弱,细胞壁处Ca^2+荧光较强,二细胞花粉时期,细胞呈现较强的Ca^2+荧光。

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