花粉

The profilin of Caryota mitis pollen was cloned and expressed successfully in E. coli BL21 (DE3), which will be used for further study on Caryota mitis pollen related allergy.

成功地克隆和表达了短穗鱼尾葵花粉profilin，为短穗鱼尾葵花粉过敏的诊断和免疫治疗奠定了基础。

The ORF of profilin of Caryota mitis pollen was amplified by RTPCR using specific primers and cloned into the expression vector pET-28a.

结果克隆获得了短穗鱼尾葵花粉profilin的全长基因，由608个碱基组成，开放阅读框为396个碱基，编码131个氨基酸。

IgE reactivity of recombinant Caryota mitis pollen profilin was investigated by western blot.

重组短穗鱼尾葵花粉profilin在大肠杆菌中高效的表达，进一步经Ni2＋亲和层析柱纯化后经Western-blot检测具有良好的免疫学活性。

The ORF of profilin of Caryota mitis pollen was amplified by RT-PCR using specific primers and cloned into the expression vector pET-28a. The recombinant Caryota mitis pollen profilin was expressed in Escherichia coli BL21 (DE3). The recombinant protein was purified by affinity chromatography using Ni(superscript 2+) coupled sepharose.

然后设计带有酶切位点的特异性引物，采用RT-PCR获得整个短穗鱼尾葵花粉profilin的开放阅读框，将其与pET28a载体连接并转化大肠杆菌BL21(DE3)进行诱导表达，通过Ni(上标 2+)亲和层析柱对重组蛋白进行纯化，采用Western-blot检测其IgE结合活性。

IgE reactivity of recombinant Caryota mitis pollen profilin was investigated by western blot. Results The Caryota mitis pollen profilin gene was cloned. The cloned fragment was 608bp in length, with an 396bp open reading frame coding for 131 amino acids.

结果 克隆获得了短穗鱼尾葵花粉profilin的全长基因，由608个碱基组成，开放阅读框为396个碱基，编码131个氨基酸。

Pollen extracts from Carvota mitis and Caryota ochlandra were prepared and analyzed by SDS-PAGE and proteins bands were visualized by staining the gel with Coomassis blue. Antigenic properties of the separated proteins were analyzed by immuno-blotting.

提取这两种花粉的粗提液，通过十二烷基硫酸钠－聚丙烯酰胺凝胶电泳分离粗提液蛋白质组分并测定其分子量。

Last May a study published by Cornell University showed that pollen from some strains of corn with built-in pesticides can kill the larva of the Monarch butterfly, a pest by nobody's standards.

去年五月，康奈尔大学的一份调查报告显示，含有杀虫功能的玉米花粉能杀死黑脉金斑蝶的幼虫，而任何人都不会把蝴蝶当成害虫。

The pollen morphology of 10 species of Scorzonera genus in the Mongo lian Plateau was observed by scanning electronic microscope.

用扫描电子显微镜观察了蒙古高原鸦葱属植物10个种的花粉形态。

The study in this paper, a Pollen-specific Lysine-rich Protein cDNA, from tomato by RT-PCR was obtained. A plant expression plasmid pBIUB-TSB was constructed and lysgene was under the control of maize ubiquitin promoter which is the highest efficient monocotyledon promoter.

本实验通过RT-PCR从番茄花粉中获得高效特异表达的高赖氨酸基因TSB的cDNA序列，构建其受控于单子叶植物Ubil强启动子下的高效表达质粒pBIUB-TSB。

Fossils of monocotyledonous pollen and stems of palms suggest that the angiosperms evolved during this period.

单子叶植物花粉和植物茎化石暗示被子植物在这个时期已有了发展进化。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。