花烟草

The plant expression vectors were constructed and two genes were transferred into A. thaliana and N. tabacum, antikanamycin plants were obtained.4. The trials of adventive bud regeneration directly and callus induction was conducted to select out the optimal explants and formula. The results revealed that double scale can produce 13~16 adventive buds per centimeter in MS medium added 10.0 mg/L 6-BA, 1.0 mg/ L NAA, 40 g/L sugar and 7.5 g/L agar. Scale, leaves, scape and different tissue of flower can be induced to produce callus in appropriate medium, but the filament with anther was the optimized explant.

构建了两个基因的pBI121和pCAMBIA1300植物表达载体，并通过农杆菌将上述基因转入烟草和拟南芥中，得到了卡那抗性的再生植株。4、以中国水仙不同部位外植体进行了不定芽直接分化和愈伤组织诱导分化试验，结果表明，双鳞片外植体在MS0+6-BA 10.0 mg/L+NAA 1.0 mg/L+蔗糖40 g/L+琼脂7.5 g/L培养基上可以大量地直接再生，再生芽数量平均可达13~16个/cm双鳞片，比传统的鳞茎盘之间分化提高30~50%；鳞片、叶片、花葶及花部不同组织在适宜的配方中均可不同程度地诱导出愈伤组织，但以带花药的花丝诱导出的愈伤组织最好。

Some annuals with good scents are sweet alyssum, the large flowering tobacco "Nicotiana Sylvestris", the Regal Lily, which flaunts clusters of trumpet-shaped white flowers, and velvety purple heliotrope.

一些一年生带香味的植物是带甜味的香雪球、最大的烟草花"薄荷烟草"、挥动着白色喇叭状花瓣的帝王百合、淡紫色的青莲。

Scented plants can also evoke memories. Some annuals with good scents are sweet alyssum, the large flowering tobacco "Nicotiana Sylvestris", the Regal Lily, which flaunts clusters of trumpet-shaped white flowers, and velvety purple heliotrope.

芳香的植物同样能唤起回忆，一些一年生植物有很好的香味，比如香雪球，大片花叶烟草植物：美花烟草，花攒锦簇，随风摇曳的白色"帝王百合"，和像天鹅绒般的紫色"青莲"。

Flowers are: loosestrife, meadowsweet, morning glory, violet, daisies, hostas Trees: all trees especially pines and spruces, Magnolia, Elder Foilage: Witchgrass, purslane, tobacco, hemp, hops Herbs: Lavender, scullcap, vervain, linden, sage Produce: avocado, rhubarb, rye.

鲜花：金钱草， meadowsweet ，牵牛花，紫罗兰，雏菊， hostas树木：所有树木尤其松树和新陈代谢，木兰，老喷施： witchgrass ，马齿苋，烟草，大麻，啤酒花草药：薰衣草，芩，马鞭草，椴，圣人制作：油梨，大黄，黑麦。

The aberrant phenotypes were observed in the anther of tobaccotransformed with ZM401 sense construct: the inflorescence growth and floral axiselongation are suppressed; the flower buds falling or flowers abscission earlier wereobserved. The degradation of tapetum are not observed at the binucleate pollen stagewhich corresponds to the mature stage, although the tapetal cells of untransformedtobacco anther at the same stage have already degenerated completely anddisappeared.

对转基因烟草进行形态学、组织学分析结果表明，ZM401基因以反向方式整合的转基因烟草表现一切正常，而含有ZM401正义基因的转基因烟草在表型和组织学上都出现了异常：花序生长缓慢，花序轴变短，生长过程中花逐渐脱落，花序轴褐化枯死；花药绒毡层退化延迟，药室内壁在雄配子体发育后期不能进行带状加厚，阻碍了花粉粒的释放。

Morning glories blow little purple bugle at 4 o'clock early morning; flamboyant rosebushes smile about 5 o'clock in the morning; nenuphars wake up from dream at 7 o'clock in the morning; peatapetes phoenicea bloom at 12:00 at noon; marigolds arrive at 3 o'clock in the afternoon; tobacco flowers wake up at 6 o'clock in the dark; moonlight flowers bloom leaves about 7 o'clock; polianthes tuberosas bloom at 8 o'clock in the evening and night-blooming cereuses appear with a smile about 9 o'clock in the evening

凌晨四时，牵牛花吹起了紫色的小喇叭；五点左右，艳丽的蔷薇(qi á ng w ē i)绽开了笑脸；七点，睡莲从梦中醒来；中午十二点左右，午时花开花了；下午三点，万寿菊 j ú欣然怒放；傍晚六点，烟草花在暮色中苏醒；月光花在七点左右舒展开自己的花瓣；夜来香在晚上八点开花；昙花却在九点左右含笑一现

Some annuals with good scents are sweet alyssum, the large flowering tobacco "Nicotiana Sylvestris", the Regal Lily, which flaunts clusters of trumpet-shaped white flowers, and velvety purple heliotrope.

一些一年生带香味的植物是带甜味的香雪球、最大的烟草花&薄荷烟草&、挥动着白色喇叭状花瓣的帝王百合、淡紫色的青莲。

Scented plants can also evoke memories. Some annuals with good scents are sweet alyssum, the large flowering tobacco "Nicotiana Sylvestris", the Regal Lily, which flaunts clusters of trumpet-shaped white flowers, and velvety purple heliotrope.

芳香的植物同样能唤起回忆，一些一年生植物有很好的香味，比如香雪球，大片花叶烟草植物：美花烟草，花攒锦簇，随风摇曳的白色&帝王百合&，和像天鹅绒般的紫色&青莲&。

Some annuals with good scents are sweet alyssum, the large flowering tobacco "Nicotiana Sylvestris", the Regal Lily, which flaunts clusters of trumpet-shaped white flowers, and velvety purple heliotrope.

香雪球，大花烟草&美花烟草&，喇叭状白色花招摇的帝王百合，以及紫色的天鹅绒向日花是香气逼人的一年生植物。

The result would be used in tobacco breeding.4 SRAP technology is used to study the genetic differences among different types of tobacco, including flue-cured tobacco, oriental tobacco, maryland tobacco, burley tobacco, and air/sun cured tobacco, five pairs of primer combinations which can amplify characteristic bands has been screened. Among the five combinations, two of them (SF25*SR2 and SF3*SR26) can distinguish air-cured tobacco including maryland tobacco and Burley tobacco from the other cultivated forms; SF20*SR14 can distinguish sun cured tobacco including oriental tobacco and sun-cured tobacco; SF16*SR14 can distinguish flue-cured tobacco and air-cured tobacco, and SF22*SR14 can distinguish DBJ599 with specisl aroma and the other flue-cured tobacco.5 8 varieties , which represent the different types of tobacco, are divided into 3 groups. I: G-28, II:DBJ599、MD40、By21, III: Basma、Sumsun、XNS、QCT. The cluster analysis result es consistant with experience evatuation.6 Anorthogonal design was used to optimize a ILP-PCR system with 5 factors (DNA, Mg2+, dNTP, primer and Taq polymerase) at 4 levels.The result showed the optimized ILP-PCR system for Nicotiana tabacum L. was: 2uL10×PCRbuffer, 20ng template DNA, Mg2+ 2.0mmol/L, dNTP 150umol/L, primer 0.3umol/L, Taq DNA polymerase 1 U in a total of 20uL reactionsolution.

研究结果可作为烤烟与香料烟类型间的杂种鉴定利用的实验与理论依据。4、利用SRAP标记技术，研究和分析了烤烟、晒烟、晾烟、白肋烟、香料烟等不同栽培类型间的基因型差异，初步筛选出了能够在分子水平上反映不同烟草类型差异的特征性标记带：晾烟MD40、Ky17、Burley21、TN90基因型共有的特征带有两对引物组合，分别为SF25*SR2和SF3*SR26；晒烟小牛舌、青梗、小花青和香料烟Sumsun、komotini Basma、Xanthi Basma共有的特征带引物组合为SF20*SR14；NC89、K326、G-28、大白筋和MD40、Ky17、Burley21、TN90共有的特征带引物组合为SF16*SR14，烤烟中大白筋599的特征带引物组合SF22*SR14.5、利用61对SRAP引物组合，研究了普通烟草不同类型间的亲缘关系，将普通烟草类型间8个不同供试材料划分为3类别。I：烤烟类型G-28II：白肋烟类型(MD40、By21、大白筋599III：香料烟类型(Basma、Sumsun、小牛舌、千层塔)。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。