芯片

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

METHODS: The paraffin-embedded specimen of 95 cases of CRC, 57 cases of lateral tissues and 25 cases of metastatic lymphoid nodes were collected and constructed as tissue microarrays. The anormal expressions of EGF, EGFR, VEGF and COX-2 were evaluated by using the S-P method of IHC.

收集1997~1998年95例CRC原发灶、癌旁组织和转移淋巴结的石蜡标本，用组织阵列仪制备组织芯片；对组织芯片进行免疫组化染色，评估不同组织中EGF、EGFR、VEGF和COX-2的表达情况。

The motion control system uses DSP LF2407A as the main processor and uses the brushless motor driving chip MC33035 and driving bridge MPM3003 to drive the blushless motor. The information detection system uses infrared sensors to detect obstacles in the environment and wireless video equipment was installed t...

运动控制部分采用以DSP LF2407A为主控芯片、以无刷电机专用芯片MC33035和驱动桥MPM3003为电机驱动控制的方案；信息检测系统采用红外传感器来检测环境障碍信息，并安装无线视频设备用以监控机器人运行环境；无线遥控系统采用摇杆式脉宽比例调节方式来实现对机器人的调速及其他运动控制。

Based on bridgeless PFC topology,a 1 500 W prototype has been designed.The control chips use L4981 and IR1150 based on average current control and one-cycle control technology.

实验基于无桥Boost PFC拓扑，控制电路分别采用&平均电流控制&技术的芯片L4981和&单周期控制&技术的芯片IR1150，研制出一台1500W的实验样机，并对两种控制电路进行了详细的对比分析。

The visualization biological chip in this invention can make the sample simultaneously accept the high efficiency laser source and uses CCD cameral select the fluorescence image of the biological chip rapidly with large area for further analysis.

本发明的显像式生物芯片仪利用准直透镜使镭射光源产生大面积的镭射光激发光源，使得各样品点同时接受高效能的激发光源，此外利用CCD相机大面积而快速的选取生物芯片的荧光影像再进行分析，可以大幅减少分析时间。

With increasing of electronic slug's frequency and development of electronic slug's integration and capsulation, the heat productivity of electronic component rapidly increases. Now the heat flow density of electronic component is up to 10~4W/m~2-10~5 W/m~2, and that it is increasing continually.

随着电子芯片频率的提高，芯片集成和封装技术的发展，电子元器件的发热量迅速提高，如今其热流密度已经达到10~4W/m~2～10~5W/m~2，并且持续增大。

This essay mainly introduces the characteristic of multifunction,and also makes a detailed introduction of the capsulation of clip,the function of the outlet,the working mode,the electrical character,the typical application circuit and the operating principle.

该文主要介绍该芯片具有多功能的特点，并对其芯片封装、引脚功能、工作模式和电气特性，以及典型应用电路及工作原理也一并进行详细介绍。

Second, a house network which based on CEBus in the low power line correspondence is built with the drive module made by interface chip of SSC P300 based on the spread spectrum technique. House network is connected to the district Ethernet to communicate with the district manage center by the embedded relay module.

其次，在研究CEBus的基础上，选用符合此协议的电力线载波芯片SSC P300作为通信接口芯片设计出驱动模块并组成家庭内部网络；家庭内部网络通过嵌入式中继模块和小区以太网实现与小区管理中心的通信。

The most popular FPGA like Cycone have adequate clok resorce to meet you demand.it has up to 8 global colk and 4 dedicate clock input plus some dual purpose clock input in.

在设计中，为了少用芯片，有时不得已要在一个芯片中用到多时钟，或行波时钟，这将带来系统的不稳定，请问有无好办法解决？

The different sealing technologies were contrastively developed for sealing chips to form close channels.

因此，对DNA提取芯片的研究已成为微流控芯片集成化和自动化的关键。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。