芥菜

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下：1、利用转基因拟南芥植株分析表明，正常生长条件下，BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达，幼嫩的荚也有表达，并随着果荚的成熟而减弱，成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导，转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导，证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点，位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明，-805/+17的启动子片段足以响应伤害和MeJA的诱导，-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明，一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的，但不足以响应MeJA的诱导，位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用，赋予BjCHI1启动子MeJA诱导性。

Planted some mustard-and-cress and radishes, and went to bed at nine.

种了一些芥菜和萝卜，9点就上床睡觉了。

Mustard-and-cress and radishes not come up yet.

4月12日芥菜和萝卜没有出来。

In the pot experiment, the lead ion concn. in the dry soils was set as 0, 100, 500, 1000, 2000 and 3000 mg/kg, the content of Pb in aerial parts and root of M. sativa and B. juncea were detected and the total migration amount of Pb, root tolerance index and enrichment coefficient were calculated.

方法］采用盆栽试验，土壤中铅离子浓度为0、100、500、1000、2000、3000 mg/kg干土，测定紫花苜蓿、印度芥菜地上部和根部的铅含量，计算铅的迁移总量、根系耐性指数、富集系数。

Dickinson was buried, laid in a white coffin with vanilla-scented heliotrope, a Lady's Slipper orchid, and a "knot of blue field violets" placed about it.

艾米丽随白色棺材下葬，棺材周围放着散发香草味的天芥菜，一株仙履兰，以及一个&蓝色三色堇花结&。葬礼在大宅图书馆进行，仪式朴素简单，时间也很短。

A handful of wild mustard, 1 bamboo shoot, 3 or 4 Tofu cakes.

野芥菜一把，笋一个，豆腐乾三，四块。

Wild mustard leaves tore into small peices.

野芥菜叶撕小片。

Wild mustard.

野芥菜。

While cooking the foods like meat , seafood these seeds of white mustard tree are used for assimilating the flavor .

而烹调的食物，例如肉类，海鲜；这些种子的白芥菜树是用于吸收味道。

The seeds can come from three different plants: black mustard , brown Indian mustard and white or yellow mustard.

芥菜籽来源于三种不同的植物：黑芥末，褐色印度芥末和白色或者黄色芥末。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷