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Based on the leave of Pistacia vera L..Qualitative and quantitative analysis of phenol from Pistacia vera L. and effect on cell ultrastructure of Pistacia vera L tissue.The extracting technology phenol content from Pistacia vera L.

本文以阿月浑子植株与外植体为材料,采用化学方法与色谱-质谱方法对阿月浑子中总酚以及酚类物质成分进行了定性和定量分析,并用透射电镜观察了外植体褐变的发生对细胞超微结构的影响。

A new method for the total analysis of the polybasic acid s and higher fatty acids in hawthorn fruit by gaschromatography-mass spectrometrywas developed.

采用硫酸甲酯化处理山楂果样品,二氯甲烷作萃取剂,用气相色谱-质谱联用法对山楂果中的多元酸和高级脂肪酸进行了全面分析。

We sample from indoor air using silonite coated canisters, preconcentrate low level samples utilizing three stages focuser,analyze VOC in indoor air with GC/MS equipment.

采用硅烷化处理内壁的不锈钢采样罐采集室内空气样品,三级冷阱预浓缩,气相色谱-质谱仪联用分析室内空气中挥发性有机物。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上,活细胞单层生长,胞浆较丰富,细胞核大小一致,有核仁×400 图2 SHEE培养细胞细胞核椭圆形,核仁较大,胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状,有伪足贴壁,表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁,呈星状或多角形,有丰富伪足和胞浆突,核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞,胞核和胞浆PI染色呈红色或淡红色,蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞,染色质凝集呈块状,右上角有一固缩细胞核TdT标记×400

By chemical and spectroscopic analysis , the five compounds were identified as isosakuranetin , 4′,7-dimethylkaempferol, eriodictyol-7-methylether, Rhamnetin, daucostorol .

采用气相色谱-质谱联用技术,对有效部位B进行分析,鉴定出34种化合物,主要为不饱和脂肪酸类和萜烯类成分,其中9,12-十八碳二烯酸乙酯的含量最大。

Schizophyllum sp. F17 can use rice hulls to produce manganese peroxidase by solid-state fermentation. Important parameters were optimized through orthogonal tests and five dyes with different structures were decolorized by applying MnP.

采用稻壳作为基质,利用裂褶菌F17固态发酵产锰过氧化物酶,通过正交实验对发酵条件进行优化,并对5种不同结构类型的染料进行脱色。

The components of impurity in sebacic acid from traditional process were analyzed by GC-MS.

采用气相色谱-质谱联用分析确定了传统工艺生产的癸二酸中的主要杂质。

A method for determination of the four metabolites of nitrofuran antibiotics such as 3-amino-2-oxazolidinone, 3-amino-5-morpholinomethyl-2- oxazolidinone, semicarbazide and 1-aminohydantoin in Poultry Tis- sues by LC-MS-MS was developed.

本文建立了用高效液相色谱-串联质谱测定禽肉组织中3-氨基-2-唑烷基酮、5-甲基吗啉-3-氨基-2-唑烷基酮、氨基脲和1-氨基-2-内酰脲4种硝基呋喃类抗生素代谢物的方法。

Therefore,it is necessary to conduct researches on the reduction of textiles dyed by those ban dyes so as to detect carcinogenic romatic amine with gas chromatography-mass spectrographic analysis.

本文对使用禁用染料的纺织品进行还原研究,并用气相色谱─质谱联用分析法检测致癌芳香胺,初步建立了一套检测纺织品中禁用偶氮染料的方法。

MAIN OUTCOME MEASURES : Culture of bone marrow stromal cells and spermatogonial stem cells and coloration and count of chromosomes.

主要观察指标:骨髓基质及精原干细胞的培养及染色体显色与计数。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。