色质

Meanwhile, cyanosis of lips, purplish tongue, varicose sublingual vein, flaccidity of lower extremities, hemianesthesia, headache, blackish eyelids, dispiritedness, tongue with ecchymosis, dysphasia, blackish complexion, squamous and dry skin, pain on the paralytic limbs, dermorrhagia, and distension of stomach and abdomen are more influential symptoms and signs.

同时，在相关的临床症状和体征的多元线性逐步回归分析中发现，唇色紫暗、舌质紫暗、舌脉曲张、下肢肌力减弱、偏身麻木、头痛，睑下青黑、精神萎靡、舌有瘀斑、言謇失语、面色晦暗、肌肤甲错、瘫肢疼痛不移、肌肤青紫/有瘀斑及脘腹胀满对脑梗塞血瘀证诊断的影响较大；将单因素分析有显著性意义的症状、体征变量，代入贝叶斯逐步判别分析，结果发现唇色紫暗、舌脉曲张、爪甲紫暗、偏身麻木、舌质紫暗、言謇失语、睑下青黑、头痛、瘫肢温度降低、下肢肌力下降等对血瘀证的诊断能力较强，而且在随后的交互检验中也显示了较好的稳定性。

Methods The LPL cDNA was isolated from the human epiploon adipose tissue by means of RT-PCR. The LPL cDNA was ligated into the pcDNA3.1Zeo. The recombinant pcDNA3.1Zeo-LPL cDNA was identified by endonucleases, PCR and DNA sequencing. COS-1 cells were transfected with the recombinant LPL gene plasmid using Lipofectamine 20001M The LPL mass in cells and the culture medium were determined by a Markit-M LPL Kit. Spectrophotometry was used to measure the LPL activity.

采用RT-PCR法从人大网膜脂肪组织获取LPL cDNA基因，以pcDNA3.1Zeo质粒作为载体，运用基因克隆技术构建野生型人LPL cDNA重组质粒，限制性内切酶酶切、PCR以及双向测序鉴定重组质粒；运用Lipofectamine 2000将重组pcDNA3.1 Zeo-LPL cDNA质粒导入COS-1细胞，RT-PCR法检测LPL mRNA，ELISA法和比色法分别检测细胞裂解液及细胞培养基中LPL浓度及活性。

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只，每组再分为高代谢脓毒症组和对照组两部分，建模方法同第一部分；2）三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸；3）灌注前抽取动脉血作为背景值，灌注达150，160，170和180min分别抽取动脉血2ml用于质谱分析；4）实验开始后前30min中每5min收集呼出气一次，随后每30min收集呼出气一次，用于气相质谱分析；5）血样经过低温离心取上清液，过阳离子及阴离子交换树脂，再经衍生化处理；6）处理过的血样进入气相色谱－质谱仪，测量其13C标记的亮氨酸、葡萄糖和乳酸丰度；7）实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度（E13CO2）；8）利用文献提供的公式算出CO2总生成率（V13CO2）以及机体各物质的通量、氧化速率以及物质代谢百分比等；9）数据分析处理采用t检验。

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现：小线角木蠹蛾性信息素分泌腺位于腹部末端8～9节之间，是一个由节间膜特化而成的上皮结构，为一可外翻的腹褶，腺体表面分布着饱满的锥形体，羽化后2天未交尾的雌蛾腺体细胞呈单层排列，腹面中央由密集的柱形上皮细胞组成，细胞排列向两侧延伸至背部，其形状由柱形逐渐变为扁平形，细胞核为椭圆形，细胞与细胞间有明显的胞连接，细胞基底膜基褶较多，质膜上分布着微绒毛，并与内表皮连接，内表皮上有多层几丁质，外角质层染色较深，细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

A HPLC/ESI-MS method studied on relative compounds of chloramphenicol succinate ;Column:an Agilent ODS column 250×2.1mm;Mobile phase: 0.05M ammonium formicate buffer PH4.0/acetonitrile=80/20 ,flow0.2ml/min, and λ276nm;Mass:trap ion mass;source:ESI;Scan mode:negative ion scan.

液相色谱条件为色谱柱：Agilent C18 2.1mm×250 mm；流动相：流动相0.05M甲酸铵用甲酸调pH至4.0 /乙腈=80/20，流速0.2ml/ min，检测波长276nm；质谱条件为离子肼电喷雾质谱仪，离子源：ESI；检出模式：负离子检测。

Gingerols in ginger oleoresin were separated by AB8 macroporous adsorption resin and silica gel column chromatography. The samples were characterized by the gas chromatography-mass spectrometry method.The peak area normalization method was used to determine the relative percentage of the components and the rate of accumulation of gingerol was determined by contrasting with gingerol standard samples. The results showed that gingerols was concentrated on the 3rd and the 4th parts.

采用硅胶柱层析和AB8大孔树脂吸脱附两种方法分离姜油树脂中的姜酚，采用气相色谱-质谱(gas chromatography-mass spectrometry， GC-MS)对分离样品进行表征，用峰面积归一化法对测试样品的气相色谱-质谱结果进行分析，确定了分离样品中各组分的相对峰面积百分比。

We discuss the method of emergency monitor ing in pollution accident which happened in water,air,and soil by using GC-MS,on account of emergency pollution accident happened increasedly and the appropriate analysis method hav't been provided.

鉴于国内突发污染事故的不断增多而相应监测方法尚未完善的当前状况，为充分发挥气相色谱-质谱联用在定性、定量中的优势，在讨论气相色谱-质谱联用在水体、大气、土壤三类有机污染事故中的应急监测方法，并针对不同的污染物情况提出了相关的前处理方法、条件程序。

The conditions of LC-MS were developed for simultaneous detection of five β-agonists in liver and muscle tissues.

研究并确定了液相色谱-质谱联用同时测量五种β-受体激动剂的液相色谱和质谱条件。

A series of thermodynamic parameters were obtained by third-order polynomial fitting Van't Hoff plots. The thermodynamic partitioning behavior of those solutes was investigated.

在288～323 K范围内测定了中性芳香族溶质在脂质体电动色谱中的分配系数，通过三项式拟合Van't Hoff图获得了一系列的热力学参数，研究了溶质在脂质体电动色谱中的热力学分配行为。

Partition coefficients of neutral aromatic solutes in LEKC between 288~323 K were measured. A series of thermodynamic parameters were obtained by third-order polynomial fitting Van't Hoff plots. The thermodynamic partitioning behavior of those solutes was investigated. The results showed that the partition coefficient became greater with the increment of temperature and the number of CH2 functional groups on benzenering.

在288~323K范围内测定了中性芳香族溶质在脂质体电动色谱中的分配系数，通过三项式拟合Van't Hoff图获得了一系列的热力学参数，研究了溶质在脂质体电动色谱中的热力学分配行为。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。