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Simultaneously he also saw chronic disease and ugliness of beautiful western HuNan life: The western of HuNan acknowledged, followed and even persevered their compressed and distorted disposition soul, the most prominent performance was ignorant and numb; Under the time extruding vigorously," simplicity, diligence ,peace, honesty " in western HuNan life etc, the outstanding innate nature was drawing back achromatically.

同时也看到了湘西生命美轮美奂之背后深潜着的痼疾与丑陋:湘西子民对性格灵魂压扁扭曲的承认,遵循,甚至坚守,最突出表现是愚昧与麻木;时代大力挤压之下,湘西生命中&质朴、勤俭、和平、正直&等优秀质素正颜消色退。

Methods The clinical data of 24 patients with neonatal organic acidemia including 20 cases of methylmalonic academia, 3 cases of propionic academia and one case of glutaric academia, were analyzed by gas chromatography-mass spectrometry.

回顾性分析24例新生儿有机酸血症的临床资料,采用气相色谱-质谱法(gas chromatography-mass spectrometry,GC/MS)进行尿或血有机酸分析。

In this paper, the chemical constituents of essential oil of Agastache formosanum Hay.,planted in Dunhuang of Gansu Province of China, have been studied by capillary gas chroma-tography-mass spectrometry.

本文介绍采用毛细管气相色谱-质谱法研究了甘肃敦煌栽培的茴霍香精油的化学成分。

BmK IT2 is worthy of being considered to be a kind of new algesia peptide drug.

BmK粗毒和BmK I诱导的大鼠脊髓背角氨基酸类神经递质的释放采用微透析采样与高效液相色谱荧光检测技术,分析BmK粗毒和其主要致死成分BmK I诱发炎症痛过程中同侧脊髓背角氨基酸含量的动态变化。

A quantitative method of alkaloidal monomers is established by HPLC/MS.The The sepatation was performed on a XTerraTM ODS column(5μm,4.6mm id×150mm) with acetonitrile -water-acetic ammonium as mobile phase at the flow –rate of 1mL/min.

建立了苦豆子中主要的生物碱单体含量的测定方法:HPLC-MS法,色谱柱选用XTerraTMODS Column(5μm,4.6mmid×150mm);流动相为乙腈-水-乙酸铵,采用梯度洗脱,流速为1mL/min,柱温30℃;质谱作为检测器,选择离子采集方式。

Amino-2-methylpropanol was used as the chemical modifying reagent of fatty acids to modify the carboxyl group into nitrogen-containing heterocycle which would effectively prevent the migration of carbon double bonds of the alkenyl in the electron impact source.

以2-氨基-2-甲基-丙醇为脂肪酸的化学修饰试剂,气相色谱-电子轰击质谱分析葵子油脂肪酸。2-氨基-2-甲基-丙醇将脂肪酸羧基修改为含氮杂环,使在EI源中避免了链烯基中碳碳双键的移动。

Chromatographic fingerprints of amomum tsao-ko in different places were determined by GC-MS, and analyzed by fingerprint similarity evaluation software.

采用GC-MS方法建立草果挥发油指纹图谱,探讨气相色谱-质谱连用技术在草果挥发油质量控制方面应用的可行性。

The acquisition method of volatile compounds, from different mechanical damage exent of oriental arborvitae, are the dynamic headspace body of improved air circulation Acquisition Act.

利用改进了的动态顶空气体循环采集法,对受不同程度机械损伤侧柏木释放的挥发物进行了采集粗提,通过热脱附冷阱-气相色谱-质谱联用仪进行了提取分析鉴定。

Methods: A HPLC-MSD method was established to determine the content of astragaloside Ⅳ in Radix astragali.

建立高效液相色谱一质谱检测器测定黄芪皂苷Ⅳ含量的方法。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组,并将阳性重组质粒,应用M13测序系统对产物进行DNA序列分析,并与MPM-129株P1基因核苷酸进行同源性比较,发现有4个位置的碱基发生了变化,其同源性为98.8%,证实了PCR所扩增DNA片段的准确性和特异性,同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异,将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针,杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应,再用BCIP和NBT呈色,制备MP DNA探针,鉴定所扩增片段的特异性,与同位素探针比较,Dig探针不受半衰期限制,可反复使用,而且价格低廉,值得推广使用。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。