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The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养,同时以P2代软骨细胞为对照组,进行组化检测老化相关β-半乳糖苷酶,阿力新蓝染色检测胞外基质硫酸GAG含量和结构,MTT比色检测增殖,免疫细胞化检测PCNA和Ⅱ型胶原,RT-PCR检测Ⅱ型胶原、Aggrecan蛋白,流式细胞仪分析细胞周期和增殖指数等方法,对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

In this work, three certified milk powder materials with different PCDD/Fs levels were Soxhlet-extracted and then cleaned up using Power-Prep system automatically. PCDD/Fs and planar PCBs were determined using isotope dilution and high resolution gas chromatography-high resolution mass spectrometry.

自动样品前处理在乳品中二恶英和共平面多氯联苯同时测定中的应用张磊,李敬光,吴永宁,赵云峰(中国疾病预防控制中心营养与食品安全所,北京 100050)摘要:采用索氏提取及自动净化处理系统对乳品进行提取和净化,在高分辨气相色谱-高分辨双聚焦质谱仪上进行定性和定量检测。

The detoxicated products of mustard with chloramine -T and chloramine -B were analyzed by gas chromatography-mass spectrometry.

用气相色谱/质谱法分析了芥子气的氯胺T、氯胺B的消毒产物。

The article studied and analysed the trace chlorophenol and nitrophenol in effluent by high performance liquid Chromatography-mass spectrometry.

对废水中微量氯苯酚、硝基苯酚进行了高效液相色谱质谱法分析研究,建立了一种简单、快速、准确测定废水中苯酚衍生物的实验方法。

Using this test chromatogram and spectrogram of fatty acid in healthy liver and liver cancer were established.

本实验得出了人体正常肝组织及肝癌组织脂肪酸的色谱图及质谱图。

A gas chromatograph with a capillary column coupled to a mass spectrometric detector is used for the analysis.

本分析使用了具有偶合质谱检测器毛细管柱的气相色谱。

This paper introduced basic principle and development of gas chromatography- mass spectrometry.

介绍了气相色谱-质谱联用分析技术的基本原理及其在纺织工业中的应用。

Can undertake nutrition of light energy inorganic is divided inside the cell of general alga contain and the light with advanced same plant adds up to green outside pigment, some kinds group still have in all special pigment and also do not show green more, so their plastid calls chromatophore especially or hold lubricious put oneself in another's position.

能进行光能无机营养一般藻类的细胞内除含有和绿色高等植物相同的光合色素外,有些类群还具有共非凡的色素而且也多不呈绿色,所以它们的质体特称为色素体或载色体。藻类的营养方式也是多种多样的。例如有些低等的单细胞藻类,在一定的条件下也能进行有机光能营养。

The Thiobarbituric acid-reactive substances assay is a sensitivity HPLC assay that measures a chromogen that is produced by the reaction of thiobarbituric acid with malondialdehyde, which is an endproduct of lipid peroxidation.

硫巴比妥酸反应物分析,利用HPLC的灵敏特性分析硫巴比妥酸与丙二醛反应所产生的发色体,其为内生性脂质过氧化物。

Rational application of rapeseed cake fertilizer and inorganic fertilizers can slow down chromoplast pigments' degradability, elevate the contents of organic acid and soluble total sugar.

采用气质联用对烤烟的质体色素降解产物和挥发性香气物质进行定性分析,并用气相色谱做了定量检测。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。