色蛋白

Combination of phosphoprotein enrichment and two-dimensional liquid chromatographic fractionation is an effective approach of phosphoproteomic studies.

磷酸化蛋白纯化技术与二维液相色谱技术的结合是研究磷酸化蛋白质组的有效方法，为下一步鉴定和研究磷酸化蛋白的功能打下坚实的基础。

Methods Deoxidize method of the trisodium of citrate was used to prepare colloid golden pellet,and label Staphylococcus aureus protein A;Using the soluble antigen of Mycoplasma pneumoniae to sensitize pyroxylin membrane,then prepare immuno- chromatographic diagnosis test paper used in clinical serum specimen detection.

方法采用柠檬酸三钠还原法制备胶体金颗粒，并标记金黄色葡萄球菌蛋白A；用支原体肺炎可溶性蛋白抗原致敏硝酸纤维素膜；组装成免疫色层析法诊断试纸，用于临床血清标本检测。

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h，MTT法检测ox-LDL对RAW264.7细胞生长的影响；流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡；用特异性siRNA沉默Daxx在RAW264.7细胞中的表达，通过AO／EB染色观察基因沉默后的细胞凋亡形态学改变；高效液相色谱检测细胞内胆固醇含量；油红O染色观察细胞内脂滴的形成情况；Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况；用Western Blot印迹法检测caveolin-1蛋白的表达。

Using fuji apple seed and new red star apple seed as the material,focuses on determination of the content of crud protein and crude fat,and analysis of physical-chemical properties and fatty acid composition in their oil.

以富士和新红星两个品种的苹果籽为原料，经索氏提取后测定了它们的粗蛋白含量、粗脂肪含量及理化性质，用气相色谱法分析了其脂肪酸组成，结果表明，富士和新红星苹果籽的粗蛋白含量分别为48.85%和49.55%，粗脂肪含量分别为23.69%和24.32%；苹果籽油的理化指标符合食用油标准，富士苹果籽油中油酸和亚油酸的含量分别为37.49%和51.40%，新红星苹果籽油中油酸和亚油酸的含量分别为38.55%和50.70%，苹果籽油具有良好的商业开发前景。

reitman method to measure ALT and AST; bilirubin oxidase method to measure total bilirubin and conjugated bilirubin; biuret method to measure serum general protein; bromocresol purple method to measure serum albumin; enzymic colorimetric method to measure serum bile acids; benzyl aldehide azo naphthol method to measure monoamine oxide.

赖氏法。测定 ALT 和 AST；胆红素氧化酶法测定总胆红素和结合胆红素；双缩脲法测血清总蛋白；溴甲酚紫法测定血清清蛋白；酶比色法测定血清胆汁酸；苄醛偶氮萘酚法测定单氨氧化酶

Serum alt,ast,and bile acid were observed by enzyme assay and the ratio of ast/alt was worked out. serum bilirubin was measured by peariman and lee method. serum albumin was tested by bromphenol assay and was simultaneously measured to hyaluronic acid, type ⅲ procollagen, laminin and type ⅳ collagen by ria. meanwhile, prothrombin time and platelet count were measured and so on. results: the level of serum aca was raised increasingly in different cirrhotic cases with different classifications of child-pugh, according to severity of the condition.

应用酶联免疫吸附法分别对不同分级的肝硬化患者血清中抗心磷脂抗体的含量进行了测定；用酶法测定了alt、ast与胆汁酸并计算ast/alt值；用peariman和lee改良法测定血清胆红素；用溴甲酚氯比色法测定血清白蛋白；用放射免疫分析法测定透明质酸、ⅲ型前胶原氮端肽、层粘连蛋白与ⅳ型胶原；并测定了凝血酶原时间与血小板计数等相关指标。

Label-free and cleavable isotope-coded affinity tag quantitative proteomic approaches were used to quantify the differential expression of secretory proteins from 3T3-L1 adipocytes with or without insulin stimulation.

首先，采用液相色谱-质谱联用方法对胰岛素刺激与否的3T3-L1脂肪细胞的分泌蛋白进行了大规模的定量分析；胰岛素刺激后，242种分泌蛋白表现为显著变化，其中55种蛋白质下调，187种上调。

The results of HPLC tests indicated that Bt toxin in Bt transgenic cotton had not obviously affected the contents of flavonoids (mainly rutin and isoquercitrin and a few quercetin ),contradictively ,the result of combined dosage test in vitro showed that that rutin had significantly enhanced the suppression of Bt-ICP on the cotton bollworm larvae., in addition to the

采用高效液相色谱法测定转Bt基因棉花主要抗虫黄酮类化合物的的变化，结果表明外源Bt杀虫蛋白表达不会对棉花自身原有的主要抗虫黄酮类物质（包括芸香苷、异槲皮苷和含量较少的槲皮素）产生不利的影响，因此可以将Bt杀虫蛋白基因转入抗虫黄酮类物质中国农业科学院博士学位论文含量高的棉花品系。

Circular dichroism spectroscopy measurements also reveal that the ordered structure content of α-helical and β-structure of ovalbumin decrease, and the unfolded random structure is enhanced with increasing pressure; While the existence of dextran sulphate to the native ovalbumin, there are no apparent change on the secondary structure of ovalbumin; It seems same to glycinin.

圆二色性光谱分析表明，经超高压处理后，鸡蛋清蛋白分子中规则的二级结构减少，而部分规则的二级结构和无规卷曲含量增加。加入硫酸右旋糖苷的鸡蛋清蛋白溶液经超高压处理后其二级结构中各部分的含量变化不大：对于大豆豆球蛋白，加入硫酸右旋糖苷后，大豆豆球蛋白分子经超高压处理后其有序结构的减少及无规卷曲的增加都相对减缓。

Under different initial total egg white lysozyme concentrations in urea solution, the refolding egg white lysozyme intermediates could be deduced to have a tendency to form a bimolecular intermediate aggregate, and this inference was further confirmed by their nonreducing SDS-PAGE and size exclusion chromatography.

当不同浓度蛋白溶菌酶在脲溶液中复性时，蛋白溶菌酶折叠中间体有形成二分子积聚体的趋势，这个推测得到了非还原电泳和体积排阻色谱结果的证实。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。