色蛋白

The present paper developed a new method in which extract analytes from seafood product s by DI water followed by precipitating proteins from extraction by trifluoroacetic acid and analyse polyphosphates in pre- pared extraction by suppressed conductivity ion chromatography whose gradient eluents were obtained by Eluent Generator.

本文建立了一种采用去离子水提取，三氟乙酸作为蛋白沉淀剂对海产品样品进行前处理，采用淋洗液自动发生装置在残产生 KOH 进行梯度淋洗，抑制型电导离子色谱法，检测海产品样品中多聚磷酸盐的方法。

The present paper developed a new method in which extract analytes from seafood product s by DI water followed by precipitating proteins from extraction by trifluomacetic acid and analyse polyphosphates in pre- pared extraction by suppressed conductivity ion chromatography whose gradient eluents were obtained by Eluent Generator.

本文建立了一种采用去离子水提取，三氟乙酸作为蛋白沉淀剂对海产品样品进行前处理，采用淋洗液自动发生装置在线产生KOH进行梯度淋洗，抑制型电导离子色谱法，检测海产品样品中多聚磷酸盐的方法。

Methods Immunohistochemical method was used and the depletion area of CTnT in myocardial ischaemia were measured by the computer image analysis.

应用免疫组化LSAB法显色观察。结果缺血15分钟，大鼠心肌肌钙蛋白T即有明显脱失，随缺血时间的延长，脱失面积呈扩大趋势。

In order to study the effects of pre-heating on the sensitivity of cells to tumor necrosis factor-α, the K562 cells were pre-heated at 40℃ for different times to induce heat shock protein 70(HSP70). To observe the effects of TNF-α concentration on the sensitivity of pre-heated cells, cells were pre-heated for 60 min and treated with TNF-α(50,100,200 and 400U/ml) for 16 hours, and the cells vitalities were detected by MTT.

为探讨预热对肿瘤细胞肿瘤坏死因子α敏感性的影响及其机理，将白血病患者胸腔渗出细胞系(K562细胞)在40℃预热不同时间(0、30、60、90、120、150和180min)，使细胞热应激蛋白70(HSP70)高表达；然后将预热60min细胞和未预热细胞分别暴露于50、100、200和400U/ml的TNF-α16小时，用MTT比色法检测细胞活力。

The results showed that the HRP-labelled pinellin was localized on the epitheliurri of endometrium and glandula as revealed after development with 3, 3'-diaminobenzidine–H_(2)O_(2), and the effect could be inhibited by unlabelled pinellin.

结果显示小鼠子宫内膜和腺管上皮以及胚胎外胚盘锥体部分呈现明显的酶标显色颗粒，它们的产生能被未经酶标半夏蛋白所抑制。

The process of the growth and morphology of the neurons were observed under phase contrast microscope in vitro.

倒置相差显微镜进行神经元形态学观察，中性红比色法和MTT法测定神经细胞的存活数量和活性，TBARS法和BCA法分别测定神经细胞中丙二醛的水平和总蛋白合成的能力。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

A novel serine protease with high purity was extracted from the venom of Agkistrodon halys Pallas using monoclonal antibody affinity chromatograpy. This proteas releases bradykinin and has arginine esterase activity without being activated.

用离子交换和亲和层析色谱法从白眉蝮蛇毒中提取出了一种高纯度的具有激肽原酶活性的蛋白水解酶，称为蛇毒激肽原酶，该酶不需活化即可水解激肽原，释放激肽。

As the invention of advanced separation techniques, it is possible to study the structures of wheat seed proteins on molecular level.

随着现代生物技术的发展以及一些先进的分离手段的出现，大大促进了对小麦种子蛋白质的结构与功能研究，其中反相高效液相色谱和生物质谱技术的发展为我们提供了贮藏蛋白快速分离与定量以及精确确定其分子量的有效方法。

The content of hydroxyproline can be determined by colorimetry ,and the content of hydrolyzed animal collagen proteins in adulterated milk was calculated by Hyp content.

采用比色法快速定量检测乳中掺加的动物胶原水解蛋白。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。