色蛋白

ResultsThe main influence factors included mobile phase,chromatogram column,detection wavelength,way of sample preparation,kind and dose of proteinic precipitator.

结果蛋白沉淀的种类及剂量、样品提取和制备方法对方法回收率及灵敏度影响较大；流动相、色谱柱、波长与流速等对分离度及保留时间影响较大。

Methods Before and after the treatment of 16 acute leukemia cases,Gomorri and Masson stainings were used to observe the change of reticulin myelofibrosis content of bone marrow.

采用Gomori染色和Masson三色染色检测16例急性白血病患者治疗前后骨髓网硬蛋白纤维含量变化。

Methods:Thirty six mice were inoculated with the sarcocarcinoma 180 according to the standard method,after 10 days,weighed the tumor and body.The expressions of bcl2 and p53 proteins in the tissue were studied by immunohistochemistry; and the activities of SOD, GSHPx and the content of MDA were detected by colorimetric method in serum.

按照标准方法给36只小鼠接种S180肉瘤后，给予珍珠梅提取物10 d，观察瘤重及体重，免疫组织化学方法检测肿瘤组织中的bcl2和p53蛋白，比色分析法检测血清SOD，GSHPx的活性及MDA含量的变化。

Methods:Thirty six mice were inoculated with the sarcocarcinoma 180 according to the standard method,after 10 days,weighed the tumor and body.The expressions of bcl 2 and p53 proteins in the tissue were studied by immunohistochemistry; and the activities of SOD, GSH Px and the content of MDA were detected by colorimetric method in serum.

按照标准方法给36只小鼠接种S180肉瘤后，给予珍珠梅提取物10 d，观察瘤重及体重，免疫组织化学方法检测肿瘤组织中的bcl 2和p53蛋白，比色分析法检测血清SOD，GSH Px的活性及MDA含量的变化。

Our data suggest that MA might modestly damage brain serotonin neurones and/or inhibit SERT protein expression, with cerebral cortex being more affected than sub-cortical regions.

我们的数据提示MA可中度损伤大脑5-羟色胺神经元和/或抑制SERT蛋白表达，其中大脑皮层较之皮质下区域受累更加明显。

Methods : MTr assay was used to examine the effect of Sorbaria sorbifolia on the proliferation of HepG-2 cell and flow cytometry was used for the cell cycle distribution.

采用MTT比色法观察珍珠梅提取物抑制肝癌HepG-2细胞增殖；采用流式细胞仪检测其对HepG-2细胞周期分布的影响，同时结合倒置显微镜、HE染色、透射电镜及免疫细胞化学方法检测凋亡相关基因bcl-2、p53的蛋白表达，观察其诱导肝癌HepG-2细胞凋亡的作用。

The expression changes of ICAM-1 and p65 mRNA level were detected by reverse transcription-polymerase chain reaction and the expression of p65 protein in AR42J cell lines were detected with Stept Avidin-Biotin complex respectively, and the changes of amylase in culture medium with chromatometry.

逆转录－多聚酶链反应半定量法观察细胞间黏附分子(ICAM-1)和p65亚单位mRNA表达的变化；链酶亲和素－生物素－过氧化物酶复合物法检测p65蛋白在AR42J细胞中的表达；人工碘比色法观察培养液上清中淀粉酶的活性改变。

The changes in the weight, exponential, structure of the tissue, cell apoptosis in testis and epididymis and the changes in the sperm tectology in epididymis were measured on the day 8, 13, 21 and 35 of the treatment,respectively,by the method of morphological examination.

分别在热应激持续到8d、13d、21d和35d时，应用形态学观察法，研究受试鼠睾丸和附睾重量及指数、组织结构、细胞凋亡和附睾精子形态学的变化；应用免疫荧光细胞化学和蛋白质印迹分析的方法，研究睾丸和附睾组织中热休克蛋白70(HSP70)的表达；应用比色法检测睾丸和附睾组织内活性氧和丙二醛、抗氧化物酶(SOD、GSH-Px、CAT)的活力或浓度的变化；应用交配试验，检测雄鼠受精能力及其致孕雌鼠的产仔数的变化，用以揭示热应激对雄鼠生殖机能的影响。

Three expression vectors containing mutant gfp which were truncated from 3′ termine were constructed in the present paper.The mutation gfp with different length under the control by T 7 promoter were expressed in E. coli BL21 cells.

本文将突变型 gfp3′末端不同程度地截短，构建成表达载体，在大肠杆菌 BL 2 1中表达了不同长度的绿色荧光蛋白，当其生色基因中 65位 Ser点突变成 Thr，则 GFP的激发波由 396nm增至 4 70 nm，不用 UV激发，仅在自然光源下就可看到强烈的绿色荧光，发光强度增加 6倍，若从 3′端截短 12个氨基酸密码，对发光无影响，但截短 75个氨基酸密码，则激发波长降到 360 nm，发光强度减少 12倍。

Five rats from both groups were killed at days 7,14 and 28,respectively.And we used deproteinization analysis to detect the plasma H2S concentration and hematoxylin and eosin stain and Mallory trichrome stain for the lung tissue.

观察指标：于试验的第7、14、28天时每组分别处死5只大鼠，采用去蛋白分析方法测量血浆中H2S含量，取肺组织行HE染色和Mallory三色染色。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。