色蛋白

MTT assay was used to assess the effects of deguelin on cell proliferation. FCM and RTPCR were used to analyze the changes of nup98 mRNA and protein in K562 cells after treating with deguelin.

用MTT比色法检测细胞增殖活性；流式细胞术及RTPCR法分析鱼藤素作用K562细胞后nup98蛋白及mRNA含量的变化。

The rate of VEGFR-3 positive expression was much higher in the esophagal carcinoma and the surrounding tissue with positive expression of VEGF-C than that in the esophagal carcinoma and the surrounding tissue with negative expression of VEGF-C.

为了探讨VEGF-C/VEGFR-3蛋白质在食管鳞癌中的表达，用免疫组织化学和Western blot来检测VEGF-C/VEGFR-3蛋白，发现VEGF-C在肿瘤细胞胞浆中表达，VEGFR-C在淋巴管内皮细胞胞浆中表达，呈棕黄色颗粒状。

Objective To extract the plasma membrane proteins from mouse liver cells and investigate the approach for fractionating the protein mixtures by two-dimensional liquid chromatography.

目的 提取小鼠肝脏细胞质膜蛋白并建立一种利用二维液相色谱法分离细胞质膜蛋白质组的方法。

Results Positive nuclei were stained diffusely or granularly brown in various intensities and all positive nuclei were counted regardless of intensity.All the esophageal squamous cell carcinoma showed positive staining PCNA.

结果 PCNA蛋白表达呈胞核内弥散分布的棕黄色颗粒，食管鳞癌呈100%表达，阳性细胞数≤25%为低表达，＞25%为高表达。

Methods:Animal models of 25 adult mixed breed dogs were established by transverse osteotomy at bilateral iliopectineal crest,which were fixed with ATMFS anterior column connector and steel plate respectively. The animals were examined at 2,4,6,8,and 12 weeks by X-ray,macropathological observation and biomechanical test. Tetracin fluorescent labeling,HE staining,Masson triad colour staining,in situ hybridization and immunohistochemical method were performed and histological image analysis were applied.

建立犬双侧骨盆弓状线骨折的动物模型，分别采用ATMFS前柱固定器和5孔重建钢板内固定，于术后2、4、6、8、12周分别行影像学检查、大体观察及生物力学测试；采用四环素荧光标记法、HE染色、Masson三色染色法、原位杂交法、免疫组织化学法进行组织学观察并图像分析；采用RT-PCR法检测骨折端不同时期骨钙蛋白和核心转录因子mRNA相对表达量的变化。

It was suggested that only if the protein is just eluted with a isocratic model.

提出了在等浓度条件下洗脱条件蛋白能够在色谱柱保留时的饱和吸附量为其有效吸附量，在这种条件下的流动相的组成，才是研究溶质饱和吸附量的合适组成。

The interaction of Jatrorrhizine and Berberine with serum albumin can induce the changes of secondary structure of serum albumin with reduction ofα-helices.

红外光谱和圆二色谱研究表明异喹啉类生物碱的加入引起人血清白蛋白二级结构的变化，降低了血清的α-螺旋结构含量。

Methods: The inhibitation of HL-60 cells by Rapamycin was measured by MTT assay. Morphic change of the apoptosis of HL-60 cells was observed by inverted microscope and light microscope, trapezoid straps by DNA agarose gel electrophoresis, and apoptosis rate by flow cytometry. Western-blot analysis was performed to detect the expression of Bax and Bcl-2 proteins after rapamycin treatment.

用MTT比色法测定雷帕霉素对HL-60细胞增殖的影响；用倒置显微镜和荧光显微镜观测细胞凋亡的形态学改变；DNA琼脂糖凝胶电泳观测凋亡的梯形条带；流式细胞仪检测细胞凋亡率；Western-blot检测HL-60细胞经雷帕霉素处理后，Bax、Bcl-2蛋白的表达情况。

Methods K-ras gene point mutation and its style at codon 12 of Patu 8988 cell were detected by using PCR-SSP and sequence analysis.

方法顺序特异引物聚合酶链反应法和基因测序检测Patu8988细胞K-ras点突变形式，根据点突变形式设计并合成硫代反义寡核苷酸（K-ras mutation ASODN）作用Patu 8988，通过噻唑蓝比色法检测细胞生长情况；流式细胞术检测K-ras蛋白表达和细胞凋亡；逆转录-聚合酶链反应检测K-ras mRNA表达水平；以Patu8988细胞建立裸鼠胰腺癌模型观察K-ras mutation ASODN在体内的抗肿瘤效果。

Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法，检测不同浓度5－FU作用不同时间对Lovo细胞生长所产生的不同影响；光镜观察凋亡细胞的形态特点；流式细胞术检测凋亡细胞百分率；琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki－67及凋亡相关基因蛋白p53、Fas表达水平。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。