色蛋白

It caused aggregation of purple membrane fragments at very low concentration. This effect might be attributed to its positive charges because cationic surfactant DTAB acted the same. On the other hand, melittin seemed to have behavior similar to CHAPS which has a rigid cholane ring,both had little effect on the conformation of retinal chromporphores.In consideration of the two-dimensional crystalline structure of purple membrane,melittin may form a rigid conformation such as α-helix on the surface of the membrane.

通过与三种在结构和电荷上不同的表面活性剂与紫膜的作用相比较，可以年出蜂毒作为带正电荷的分子与同样带正电的表面活性剂DTAB在引起紫膜凝聚方面表现相同；但在对紫膜可见光区的吸收光谱和圆二色谱的影响上却与具有刚性结构的CHAPS相似，表明蜂毒可在紫膜表面以一种刚性较大的构象存在，不能进入膜蛋白流水区的深层。

Methods The plasma levels of protein C activity and protein C antigen were measured using chromogenic assay and ELISA, respectively.

分别用发色底物法和ELISA测定血浆蛋白C活性和抗原。

MethodsThe cells of human gastric cancer cell line SGC7901 was treated respectively with Chrysin at different concentration 10、20、40、80μM for 48h. The proliferation inhibitory rate was measured by MTT assay. Acridine orangestaining and flow cytometry were used to detect apoptosis. Western blot assay was used to detect the expression of apoptosis related genes NF拨B,Bcl2,Bax.

方法分别用10、20、40、80μM的ChR处理人胃癌细胞株SGC7901 48h，采用MTT比色法检测ChR对SGC7901细胞的增殖抑制效应；采用丫啶橙染色、流式细胞术检测ChR诱导SGC7901细胞凋亡的发生；应用Westernblot法检测凋亡相关基因NF拨B、Bcl2、Bax的蛋白表达，并用计算机图像分析软件进行半定量分析。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of 5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophageal ganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied by Streptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embedded serial sections.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmonia axyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophagealganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied byStreptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embeddedserial sections. An overall observation reveals that the brain structure of the beetle is relativelydistinct.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmoniaaxyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

A simple, rapid and accurate method for the determination of hydroxyproline in collagenous proteins by high performance liquid chromatography-electrospray mass spectrometry (HPLC-ESI/MS) was developed.

建立了一种简单、快速地测定胶原蛋白中羟脯氨酸的高效液相色谱-电喷雾质谱(HPLC-ESI/MS)方法。

Moreover, the proposed method has good selectivity as well. Most metal ions and protein et al. can be tolerated in considerable amounts. It has been applied to determine calcium in in the cytolymph of industrial microorganis with satisfactory results.

此外，该显色体系还具有良好的选择性，大多数常见金属离子、蛋白等活性成分均有较高的允许量，用于生物胞液中Ca2+的直接测定，结果令人满意。

Methods:Use methods such as take off protein,calcium,colour,deacetylate and purification to prepare chitosan to study deacetylate degree,molecular weight and other characters of chitosan,then compare with infrared spectrum of bio-chemical chitosan on market.

用脱蛋白、除钙、脱色、脱乙酰基、纯化等方法制备壳聚糖，研究其壳聚糖的脱乙酰度、分子量等性质，并与市售生化级壳聚糖的红外光谱进行比较。

objective:with the local lobster shell as the raw material,we inquire into the abstraction and preparation of chitin and chitosan to develop a new raw material resources for chitosan.methods:use methods such as take off protein,calcium,colour,deacetylate and purification to prepare chitosan to study deacetylate degree,molecular weight and other characters of chitosan,then compare with infrared spectrum of bio-chemical chitosan on market.results:the chitin and chitosan obtained from lobster shell are 14.8% and 8.9%, respectively.

目的：以地产龙虾壳为原料，探讨其甲壳素的提取和壳聚糖的制备方法，以开发壳聚糖制备的新原料资源。方法：用脱蛋白、除钙、脱色、脱乙酰基、纯化等方法制备壳聚糖，研究其壳聚糖的脱乙酰度、分子量等性质，并与市售生化级壳聚糖的红外光谱进行比较。

The invention discloses a method for extracting polysaccharide from chiretta and an application, which comprises breaking dry chiretta via a breaker into powder, arranging into an extraction pot, adding ligarine to reflux and defat, adding distilled water into defatted residues to extract, processing alcohol precipitation and centrifugation to obtain deposits, removing protein, processing acid hydrolysis and gel chromatography separation, freezing and drying to obtain active chiretta polysaccharide.

本发明公开了一种从当归中提取多糖的方法及应用。干燥当归用粉碎机粉成粉末，置入提取罐中，加入石油醚回流脱脂，向脱脂后的残渣中加入蒸馏水提取，醇沉、离心得沉淀，除去蛋白、酸解、凝胶色谱分离、冷冻干燥得到活性当归多糖。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。