色蛋白

For it is rubor after damaging to the closing soft tissue, getting swollen, getting hot aching and model of functiolaesa these bed solicit and there is no fracture,no skin damaged, we designed the corresponding experiment, namely the law of hot board causes little mouse\'s painful experiment of threshold of pain; Freun\'s complete adjuvant caused the swollen experiment of big mouse\'s foot;The xylene caused the swollen experiment of little mouse\'s ear; Invigorating blood circulation silt experiment that changes the sex that the rheology appearance examines the blood and flows with the blood; Useing the high performance liquid chromatography-electrochemistry Detected monoamine neurotransmitters transmitter in the brain and serum; the experiment of immunohistochemistry measured the proto-oncogene C-FOS expresses.

针对闭合性软组织损伤后的红、肿、热、痛及功能障碍的典型临床体征，加上无骨折、无皮肤破损的特点，我们设计了相应的实验，即热板法致小鼠疼痛的痛阈实验；福氏完全佐剂(Freun\'s complete adjuvant，FCA)致大鼠足肿实验；二甲苯致小鼠耳肿实验；用血流变仪测血液流变性的活血化瘀实验；运用高效液相色谱—电化学(high performance liquid chromatography-electrochemistry Detection HPLC-ECD)法测量大脑及血清中单胺类神经递质的实验以及免疫组织化学法检测原癌基因C-FOS蛋白产物表达的实验。

Methods The β-sitosterol and/or total protein of Pinella were incubated at different concentrations with cervical cancer cell line SiHa. The effects of β-sitosterol and/or total protein of Pinella on the viability of cells were tested by MTT assay.

在子宫颈癌细胞SiHa的培养基中加入不同浓度的β-谷甾醇和／或掌叶半夏总蛋白培养1~7d，采用MTT比色法检测细胞的活性。

The distance between donor and amptor calculated by Forster theory agress with the dimensions of R-PE and APC.

根据Forster能量转移机理，计算得出给体与受体发色团间距离为72A，这一距离与两种蛋白的大小是基本相符的。

A series of study methods which can not research in this field begin to exert important affection. On the basis of the previous research, the following major innovative work was carried out in this dissertation.(1) Several methods including fluorescence spectra, CD and FT-IR specta methods were used to study the interaction of isoquinoline alkaloid、isoflavones and Rhein with serum albumin, and the results from every method was in good agreement;(2) The binding constants、the numbers of binding site、the binding region and the interaction force involving in the interaction of drugs and serum albumin were confirmed by Scatchard equation and Vant Hoff equation.

基于研究生物大分子与小分子相互作用具有极其重要的意义，本博士论文在前人工作的基础上，主要进行了以下几方面的创新性研究：(1)利用荧光光谱法、圆二色谱法、红外光谱法以及分子模拟等多种方法相结合研究了异喹啉类生物碱，异黄酮类化合物以及大黄酸与人血清白蛋白的相互作用，获得了准确一致的信息；(2)根据Scatchard方程和Vant Hoff方程详细研究计算了异喹啉类生物碱以及大黄酸与蛋白质结合机理包括结合常数、结合位点数、结合区域、结合力。

Chlorophyll fluorescence analysis technique and HPLC system were used to investigate changes of thermal dissipation through xanthophyll cycle under high irradiance and xanthophyll cycle components in streptomycin treated maize leaves.

利用叶绿素荧光分析技术和高效液相色谱研究了链霉素(SM ，叶绿体基因编码蛋白的抑制剂)处理玉米叶片的叶黄素循环及依赖叶黄素循环的热耗散。

This article introduced the application of thiophilic adsorption chromatography in isolation of the enzyme from Agkistrodon acutus venom and the characters of the enzyme such as anticoagulant activity and proteolytic activity were identified.

本文介绍了应用嗜硫色谱提取薪蛇蛇毒中蛋白组分的效果，并对该组分进行部分表征，鉴定了谈组分的抗凝血效果和蛋白酶活性。

The object of this study is to evaluate the immunoregulation and anti-tumor activities of Arnebia root polysaccharide in vitro. The ARPS was obtained by hot-water extraction, alcohol precipitation, de-protein, de-color, and dialysis etc. from Arnebia root and was administered on mouse spleen lymphocyte and tumor cell with 24 μgmL^(-1), 121 μgmL^(-1), 364 μgmL^(-1), respectively.

为研究紫草多糖在体外对小鼠脾淋巴细胞的免疫调节活性及对肿瘤细胞的细胞毒作用，本实验采用水提醇沉法，经脱蛋白、脱色、透析等方法提取紫草多糖，MTT法检测紫草多糖对淋巴细胞增殖及对HepG2、SPC-A-1肿瘤细胞的杀伤作用。

Potassium, natrium determines it is good that use ion chooses electrode indirect method it is better also with indirect method to determine at calcium of direct way;, it is next occasionally phosphor of; of law of azotic arsenic Ⅲ determines use phosphor molybdic acid is ultraviolet law outcome is better it is better with result of law of personal candy kinase that; blood sugar determines; urea determines use Niao enzymatic ultraviolet anhydride of better; flesh uses rate law result enzymatic law outcome is better; albumin determines, it is vanadium acid next alkalescent phosphoric acid of oxidation law; is enzymatic determine outcome of fluid of use AMP amortize is better; amylase determines law of thing of enzymatic standard background and iodic colorimetric law coefficient of variation all bigger, former a bit small, latter of percent of pass is tallish, each lab can try to choose according to his condition.

结论钾、钠测定使用离子选择电极间接法好于直接法；钙测定用间接法也较好，其次是偶氮砷Ⅲ法；磷测定使用磷钼酸紫外法结果较好；血糖测定用己糖激酶法结果较好；尿素测定用脲酶紫外速率法结果较好；肌酐用酶法结果较好；白蛋白测定用溴甲酚绿法较好；胆红素测定用酶法结果好，其次是钒酸氧化法；碱性磷酸酶测定使用AMP缓冲液结果较好；淀粉酶测定酶法底物法和碘比色法变异系数均较大，前者稍小，合格率后者稍高，各实验室可根据自己的条件加以选用。

Methods The BiVP gene was screened by the ESTs technology and cloned by PCR method from the cDNA library of the bumblebee, Bombus ignitus. It was reconstructed to form the baculovirus transfervector pBac1-BiVP and expressed in Sf-9 insect cell. The recombinant BiVP was purified by the fast protein liquid chromatography system.

利用ESTs从红光熊蜂cDNA文库中获得BiVP基因，以PCR技术扩增出BiVP的完整cDNA，并构建病毒表达载体PBac1-BiVP，通过昆虫杆状病毒表达系统，在Sf-9昆虫细胞中进行表达，表达产物用快速蛋白液相色谱系统进行纯化。

Method8: The paPef, fOr the first time, studied the apoptosis or necrosis of K562cclls induced by bungarus multicinclus crude venom and several components frOmcrude venom by cation exchange chromatography\' through the quatitative analysis offlow cytometry as well as the qualitative analysis, such as inversed opticalmicroscopy, MTT method and fluorescent microscopy, and so on.

本卖验采用羧甲基纤维素C-52阳离子交换层析将银环蛇粗毒进行了若干毒素组分的分离纯化，并以流式细胞术定量分析为主，以倒置显微镜、MTT比色法、荧光显微镜等多种定性分析为辅，首次对银环蛇粗毒及其若干组分蛋白对人慢性粒细胞白血病K562肿瘤细胞的细胞杀伤作用和凋亡诱导情况进行了研究。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。