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Methods Thiobarbituric acid colorimetry was used to detect the effects of Glutathion,2-carboxyethyl germanium sesquioxide (Ge-l32) and ascorbid acid on methylmercury-induced LPO in vitro.

方法运用硫代巴比妥酸比色法,检测了谷胱甘肽、羧乙基锗倍半氧化物(Ge-132)、维生素C等对甲基汞在体外诱导的大鼠脂质过氧化产物的影响。

Unguis Hominis METHOD:The lipids are extracted by solvent method,then esterified,and identified by capillary gas chromatography mass spectra computer technique.

溶剂法提取脂类成分,经酯化后,采用气相色谱-质谱-计算机联用技术分离鉴定,归一化法计算相对百分含量。

Unguis Hominis ; lipids ; squalene ; GC/MSD Analysis

人指甲;脂类;角鲨烯;气相色谱-质谱分析

Methods The separation technique of membrane-filtering an d gel chromato- graphy was used to inspect drug-loaded mechanism of SM-SLN prepared by different methods.

采用膜滤法和凝胶柱色谱法对不同方法制备的水飞蓟素固体脂质纳米粒的载药形式进行研究。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的,我们设计了以下的实验步骤﹔(1)二次代谢物回收及检测样本处理:我们将发酵所得的培养上清液,利用疏水吸附性树酯Amberlite XAD-2吸附回收,并以甲醇溶离及真空减压浓缩脱水等方式处理,以取得提供抗氧化活性筛选之检测样本;(2)抗氧化活性检测及菌种筛选:以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用;经此筛选程序,我们选获了具有抗氧化物质高生产力的菌株,命名为AMBL-029C;(3)抗氧化物质的分离纯化:针对生产菌株的发酵回收处理液,以矽胶薄层色层分离法经物质层析纯化后,并以高效能液相层析法(High performance liquid chromatography;HPLC)分析物质可得一初级纯化物质,命名为AMBL-029C-TS;(4)抗氧化物质的物理化学性质分析:由酸碱转溶(acid-base fractionation)实验得知,此抗氧化物质属於中低极性的强酸性物质,对温度(37℃-100℃)及酸碱度(pH3.0-13.0)均表现出高稳定性;(5)在抗氧化机制探讨方面,我们针对数种不同的抗氧化机制进行探讨,即: 1。

MethodsWith chloroform as solvent, the liposoluble constituents in Ligularia fischeri were extracted by ultrasonic extraction. The extracted substances were isolated and identified by GC-MS.

方法以氯仿为溶剂超声提取蹄叶橐吾的脂溶性成分,利用气相色谱-质谱联用仪对提得物质进行分离和鉴定。

Malassezia can breakdown lipid like esterase,and result in morphological changes of malpighian cell and cell apoptosis,these are all their main etiological factors.

马拉色菌能像酯酶一样分解脂质,而且可引起角质形成细胞形态学改变和细胞凋亡,这些都是它的主要致病因素。

The hydroxyl free radical scavenging rate was determined by phenanthroline spectrophotometry, and the antilipoperoxidant was determined by colorimetry.

采用邻二氮菲分光光度法测定经自由基的清除率,比色法测定其对脂质过氧化的抑制作用。

Both healthy rabbits and rabbits with glass implanted in their vitreum were involved.

作者应用气相色谱法测定健康兔和眼球内埋玻璃块的兔视网膜脂质中脂肪酸的组成和含量。

A series of analogs of fatty acid amide and fatty nitrile have been identified in shell bar section, Qaidam Basin, Tibetan Plateau by using the gas chromatography-mass spectrometry.

中文摘要:利用气相色谱质谱联用仪对青藏高原柴达木盆地察尔汗湖区贝壳堤剖面古湖相沉积物样品进行了系统地分析,检测出系列脂肪酸酰胺与脂肪腈类含氮类脂物分子化石。

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Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。