色联

In this article, we studied some components in giant panda seminal plasma and their relationship with semen characteristics.The protein bands were separated by SDS-PAGE, platelet-activating factor acetylhydrolase activity and glutamic-aspartic aminotransferase activity were detected by ELASE and chymic colorimetry respectively. The results are as followed:Twelve protein bands in giant panda seminal plasma were identified by SDS-PAGE, the molecular weights were 74.9、59.6、 56.2、 54.7、 47.3、 38.2、 30.8、 23.8、 18.4、 16.8、14.0 and 13.4kDa respectively, six of them (74.9, 59.6, 56.2, 47.3, 30.8 and 13.4kDa) werepresent in all samples. However, there was no relationship was found between the abnormalprotein bands appeared in several samples and semen characteristics in our experiment.

本研究采用SDS-PAGE对大熊猫精浆蛋白电泳谱带特性进行分析，使用酶联免疫方法检测精浆血小板激活因子乙酰水解酶活性、化学比色法检测精浆谷草转氨酶活性，并初步探讨了这些物质与大熊猫精液质量的关系，结果显示：(1)大熊猫精浆SDS-PAGE电泳共分离得到12条蛋白谱带，其分子量分别为74.9kDa、59.6kDa、56.2kDa、54.7kDa、47.3kDa、38.2kDa、30.8kDa、23.8kDa、18.4kDa、16.8kDa、14.0kDa和13.4kDa，其中74.9kDa、59.6kDa、56.2kDa、47.3kDa、30.8kDa、13.4kDa谱带为所有个体大熊猫精浆样品所共有。

With the application of Partial Least Square technique and the input need of only 2-D molecular structure, it overcomes the difficulty of conformer alignment for 3D-QSAR techniques and avoids the multiple collinearity for traditional Multiple Linear Regression.

本文应用基于分子全息的QSAR技术研究了联苯以及209种氯代同系物的水溶解度、正辛醇水分配系数、总分子表面积、水活度系数和亨利常数等5种与生物累积性有关的理化性质以及气相色谱相对保留时间，建立了一批具有高度预测性的QSAR模型。

By releasing the cytosol proteins with Digitonin and centrifugation technology, we divided the J6-2 cell proteins into the cytosol proteins and the particulate-conjugated proteins, and then using SDS-PAGE, Western blotting and Enzyme-linked immunosorbent stain combined with computer quantiscan technology, we investigated the effect of GM3 on PLC1 translocation.

本文采用洋地黄皂甙增加细胞膜渗透性，释放胞液蛋白，通过离心将细胞蛋白分为胞液蛋白和颗粒结合蛋白两部分，然后采用SDS-PAGE，Western blot及酶联免疫显色技术，并结合计算机扫描半定量技术，检测了GM3处理前后PLC1在细胞内的转位情况。

It is found that the dihedral angle in the two kinds of molecules were affected by the formation of the nanotubes, as a result, the CD spectra are different.

制备了手性分子二—2—萘基吡咯醇及--2，2'-甲氧基-1，1'-联二萘的纳米管，发现纳米管结构的形成对手性分子的二面角产生一定影响，进而影响其圆二色谱。

In this paper,the residue analysis of three preservatives—diphenyl, 2-phenylphenol and thiabendazole in fruits and vegetables by RP-HPLC is described.

反相高效液相色谱法测定水果、蔬菜中的防腐剂联苯、邻苯基苯酚和噻苯咪唑的残留量。

It comprises following steps: dissolving cross linker, functional monomer, mode molecule and initiator into pore-generating agent, getting mixing solvent; hypersonic de-gassing the mixing solvent; venting nitrogen after even mixing, sealing at nitrogen condition or vacuum condition; proceeding polymerization reaction, employing heat initiation for molecule engram polymer; grinding got molecule engram polymer after polymerization, sifting and removing mode molecule with organic solvent, checking with high efficiency liquid chromatography- ultraviolet until there is no mode molecule; vacuum drying and getting final product.

具体为：首先将交联剂、功能单体、模板分子和引发剂溶解在致孔剂中，得到混合溶剂；将混合溶剂超声脱气；混合均匀后，通入氮气，氮气状态或抽真空状态下密封；进行聚合反应，分子印迹聚合物采用热引发；聚合反应结束后，将合成的分子印迹聚合物取出研磨、过筛后用有机溶剂除去模板分子，通过高效液相色谱-紫外检测，直到检测不到模板分子；将除去模板分子的聚合物，真空干燥，得到利血平特异的分子印迹聚合物。

Methodology: Random sampling in the designated sites in the vegetable producing bases was conducted to collect 120 samples; Gas chromatography and gastity joining flocculus were adopted to test residues of 13 kinds of agro-chemicals such as organic Phosphorus,organic Chlorine and Dalmatian Chrysanthemum Ester remaining on vegetables from the producing bases, free markets and wholesale markets of Hezhou ciaty.

采用随机与定点抽样的方法采集贺州市主要蔬菜生产基地及农贸、批发市场中蔬菜样本120份，采用气相色谱和气质联谱法测定其中13种有机磷、有机氯和拟除虫菊酯类农药残留。

Four kinds of endogenous phytohormones in latex of Hevea brasiliensis were tested by using PGRIA and Gaseous Chromatogram.

采用酶联免疫检测技术和气相色谱法测定了巴西橡胶树胶乳中生长素、细胞分裂素、脱落酸和乙烯等4种植物生长物质的含量。

Different concentrations of glucose (0.10%, 1.50%, 2.50%, and 4.25%) and LPS (0, 0.1, 1.0, 10.0, 50.0, and 100.0 mg/L) were added into culture media. Then PMCs were incubated in culture medium containing 10.0 mg/L of LPS and 4.25% of glucose for 0, 1, 3, 6, 12, and 24 hours. The hexokinase activity of PMCs was measured with standard glucose-6-phosphate dehydrogenase (G6PDH)-coupled enzymatic assay.

首先将含不同浓度的葡萄糖（0.10%、1.50%、2.50%、4.25%）分别和不同剂量的LPS（0、0.1、1.0、10.0、50.0、100.0mg/L）加入培养基；其次用含4.25%葡萄糖和10.0mg/L LPS的培养基孵育细胞不同时间（0、1、3、6、12、24h）；6-磷酸葡萄糖脱氢酶耦联比色法测定HK活性。

The level of tumor necrosis factore alpha, interleukin-6 (IL-6) and interleukin-8 (IL-8) were evaluated using ELISA method. Western blotting was used to detect the protein expression of protein kinase B and lipid phosphatase PTEN. Immunoprecipitations and radioactivity of γ〓P incorporated into its specific substrate histon H〓B was utilized to determine the PKB activation. Phosphate concentration released from PTEN-specific substrat diC16PIP3 was assessed using green reagent method.

以细胞计数、噻唑蓝比色法测定细胞增殖能力，酶联免疫法检测培养上清液中细胞因子TNFα、IL-6、IL-8水平，Western免疫印记定量蛋白激酶B、蛋白磷酸酶PTEN表达水平，免疫沉淀、特异底物组蛋白H2Bγ〓P掺入量测定PKB活性，特异底物dic16PIP3脱磷酸绿色试剂法反应PTEN活性。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了