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色素蛋白

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Mutant libraries created by saturation mutagenesis of each single amino acid site D168, A225, K434 and E435 were screened to further improve the capability of cytochrome P450 BM-3(A74G/F87V/L188Q) mutant from Bacillus Megaterium which can hydroxylate indole into indigo. Results showed that D168, K434 and E435 are located at the functional domain of P450 BM-3 protein while A225 is sited at the unfunctional domain.

为了进一步获得具有更高活力的细胞色素P450 BM-3(F87V/A74G/L188Q)突变酶,利用饱和突变技术对该突变酶的4个氨基酸位点D168、A225、K434、E435分别进行单一的随机突变,通过对其羟基化吲哚生成靛蓝的催化性能表征,发现P450 BM-3氨基酸残基的168、434和435位均位于蛋白功能区域,而225位则位于非功能区域。

In this paper, the effects of increased concentrations of pyrene (0, 0.1, 1 and 10 mg/L) on the photosynthetic pigments, net photosynthetic rate, soluble sugar, soluble protein content ,and water metabolism of mangrove Kandelia candel Druce seedlings were studied in sand culture.

采用砂基栽培,研究不同浓度(0, 0.1, 1, 10mg/L)多环芳烃芘,对红树植物秋茄幼苗光合色素含量、光合速率、可溶性糖和可溶性蛋白含量以及水分代谢的影响。

The influence of three kinds of heavy metal on the activities of peroxidase, superoxide dismutase,catalase and the contents of malondialdehyde, soluble protein and chlorophyll of Lemna minor was investigated.

对水体中主要的3种重金属对青萍抗氧化酶系统的几种酶,包括过氧化物酶、超氧化物歧化酶、过氧化氢酶的活性,丙二醛的含量,可溶性蛋白含量及叶绿体色素含量等指标的影响进行了研究。

The content of levulinic acid in soy sauce,hydrolyzed vegetable proteinand caramel was determined.

对酱油、酸水解植物蛋白、焦糖色素中乙酰丙酸的含量进行了测定。

The oxidase system includes both membrane-bound and soluble cytosolic proteins. The primary feature of this enzyme is stimulus-dependent activation and which mechanism is the phosphorylation and translocation of a complex of cytosolic factors to the membrane, where they associate with a flavocytochrome enzyme.The fully assembled complex functions as an electron transport system,moving electrons from cytosolic NADPH to molecular oxygen to form superoxide anion(O2-), which, along with subsequent reactive products called respiratory burst,exerts microbicidal and cytotoxic activities.

此氧化酶体系包括膜结合的和可溶的胞浆蛋白,其主要特征为依赖刺激而活化,机制为复合体的胞浆因子磷酸化并转位至膜,在这里与细胞色素酶相关联,组装完全的复合体在功能上作为一个电子转运体系,从胞浆NADPH转移电子给分子氧形成超氧阴离子(O2-),伴随着接下来的活性物质产生,也叫呼吸爆发,发挥抗菌和抗细胞毒素的活性。

In order to explore the role of cytochrome c oxidase during the development of Microtus brandti, the activity of mitochondrial CcO and content of mitochondrial protein in voles' liver was measured by using ultraviolet spectrophotometry, these voles were chosen according to days.

为探讨线粒体能量代谢中细胞色素c氧化酶(cytochrome c oxidase,CcO)在布氏田鼠生长发育过程中的作用,应用紫外分光光度法测定了不同日龄的幼鼠肝脏内线粒体蛋白含量及CcO活性。

At the same time, such protein molecula alterations would be useful to discover the important influence of heme axial ligands on the protein's structure and properties.

为了实现细胞色素b〓的人工设计和分子改造,通过蛋白质工程方法打破这种均衡的六配位结构,创造出可能的底物结合空穴,将可望构建出具有催化活性的新金属蛋白分子。

As they report in the Public Library of Science, HenrikMouritsen of the University of Oldenburg, in Germany, and his colleagues, thinkthat proteins called cryptochromes are involved.

德国奥尔登堡大学的亨利克·莫里特森及其同事们在《公共科学图书馆》杂志上发表的报告认为,这与一种名叫隐色素(亦称蓝光受体, cryptochrome )的蛋白有关。

Microbubbles attached with the naked plasmid DNA of EGFP were infused into the rat by periocular injection with destructing microbubbles by ultrasound immediately. After 2 weeks , the EGFP expression in the rats'retina were detected by confocal laser scanning microscopy .

两周后处死大鼠做冰冻切片,在激光共聚焦显微镜下观察蛋白表达情况及分析EGFP质粒在大鼠色素上皮层的表达强度。

This technology of puvifying polysaccharide of Polygonatum cirrhifolium root was investigated.

对卷叶黄精多糖的醇沉分离、脱蛋白和脱色素工艺进行了研究。

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